Effects of light-emitting diodes on protoplast regeneration from gametophytic cells of the commercial kelp <italic>Undaria pinnatifida</italic> (Laminariales, Phaeophyceae)

Abstract

Light-emitting-diodes (LEDs) are a lighting source useful for the precise evaluation of light quality effect on biological systems. Despite the importance of light spectra on the regeneration of land plant protoplasts (“naked cells”), this factor has not been tested yet on protoplasts from multicellular algae. This study reports on the effects of pure primary colors (red, blue, and green), dichromatic (red plus blue, RB, 1 : 2) and white LEDs on protoplast regeneration from male and female Undaria pinnatifida gametophytes. We also evaluated the effect of different light spectra on pigment composition (chlorophyll a, chlorophyll c, and fucoxanthine), and the light intensities under the best condition on the regeneration process. In the early stages, blue or RB LEDs increased the percentage of dividing female protoplasts, whereas red, blue, and RB LEDs enhanced that of dividing male protoplasts. In the later stages, RB LEDs showed a positive effect only on the percentage of multiple rhizoid-like protrusions (male gametophyte). They also increased the final area of both regenerated gametophytes. The LEDs did not affect pigment composition in female gametophytes. In male gametophytes, in contrast, they reduced chlorophyll c, while blue, RB, and green LEDs decreased fucoxanthin. Under RB LEDs, the optimal light intensity was 80 μmol photons m-2 s-1 for female gametophytes and 40 to 60 μmol photons m-2 s-1 for male gametophytes. Our results suggest that dichromatic LED illumination (red–blue) improves regeneration of U. pinnatifida gametophyte-isolated protoplasts. Thus, dichromatic LEDs might a suitable light source for enhancing protoplast regeneration in brown seaweeds.

Regulation of photosynthetic and hemolytic activity of Phaeocystis globosa under different light spectra

“…Blue light is dominant in aquatic systems and can act as a stressor for some phototrophic organisms, as shown by the inhibition of growth in Haematococcus pluvialis (Xi et al ., 2016) and Synechocystis sp. (Luimstra et al ., 2018), fucoxanthin production in Undaria pinnatifida (Avila‐Peltroche et al ., 2022), Chl a production in Porphyra leucosticte (Korbee et al ., 2005), Chl C 2 in Emiliania huxleyi (Garrido et al ., 2016), and fucoxanthin in P. globosa (Fig. 5d).…”