Effects of lentivirus-mediated CCR3 RNA interference on the function of mast cells of allergic rhinitis in mice

Wu, Shuhong; Tang, Siyi; Peng, Haisen; Jiang, Yinli; Liu, Yuehui; Wu, Zhonghang; Liu, Qinqin; Zhu, Xinhua

doi:10.1016/j.intimp.2019.106011

Cited by 13 publications

(9 citation statements)

References 39 publications

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“…The lentivirus infection vector si-H19 and si-NC were injected into the nasal cavity of newborn mice according to the instructions provided. 15 Each mouse was dripped twice with 5 μL lentivirus infection vector, respectively, at a 3-hour interval. Then Hyp treatment was initiated and lung tissues of newborn mice were collected on the 14th day after birth for subsequent detection.…”

Section: Methodsmentioning

confidence: 99%

Mechanism of lncRNA H19 in Regulating Pulmonary Injury in Hyperoxia-Induced Bronchopulmonary Dysplasia Newborn Mice

Zhang

Wang²,

Shen

et al. 2020

Am J Perinatol

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Objective Bronchopulmonary dysplasia (BPD) is a pulmonary injury related to inflammation and is a major cause of premature infant death. Long noncoding RNAs (lncRNAs) are important regulators in pulmonary injury and inflammation. We investigated the molecular mechanism of lncRNA H19 in pulmonary injury and inflammation in hyperoxia (Hyp)-induced BPD mice. Study Design The BPD newborn mouse model was established and intervened with H19 to evaluate the pathologic conditions and radial alveolar count (RAC) in lung tissues of mice in the room air (RA) and Hyp group on the 4th, 7th, and 14th days after birth. The levels of BPD-related biomarkers vascular endothelial growth factor (VEGF), transforming growth factor β1 (TGF-β1), and surfactant protein C (SPC) in lung tissues were detected on the 14th day after birth. The expression of and relationships among H19 and miR-17, miR-17, and STAT3 were detected and verified. Levels of interleukin (IL)-6, IL-1β, p-STAT3, and STAT3 levels in mouse lung tissues were detected on the 14th day after birth. Results Hyp-induced mice showed increased alveolar diameter, septum, and hyperemia and inflammatory cell infiltration, upregulated H19, decreased overall number and significantly reduced RAC on the 7th and 14th days after birth, which were reversed in the si-H19-treated mice. VEGF was upregulated and TGF-β1 and SPC was decreased in si-H19-treated mice. Moreover, H19 competitively bound to miR-17 to upregulate STAT3. IL-6 and IL-1β expressions and p-STAT3 and STAT3 levels were downregulated after inhibition of H19. Conclusion Downregulated lncRNA H19 relieved pulmonary injury via targeting miR-17 to downregulate STAT3 and reduced inflammatory response caused by p-STAT3 in BPD newborn mice. Key Points

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Section: Methodsmentioning

confidence: 99%

Mechanism of lncRNA H19 in Regulating Pulmonary Injury in Hyperoxia-Induced Bronchopulmonary Dysplasia Newborn Mice

Zhang

Wang²,

Shen

et al. 2020

Am J Perinatol

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“…Some studies have shown that SIRT1 changes cell activity and affects its intracellular function by deacetylating the target protein in the nucleus (24), invasion and metastasis of malignant tumors are closely related to the up-regulation of SIRT1 expression, and down-regulation of SIRT1 inhibits the growth of liver cancer and ovarian cancer cells (25). In other studies, the inhibitory effect of shRNA lentivirus vector on CCR3 gene expression could effectively reduce the migration, infiltration and degranulation of mast cells in local tissues, and reduce inflammation in allergic rhinitis mice (26). The results in this study further supplemented the biological function of SIRT1 on breast cancer cells by establishing a shRNA lentivirus vector.…”

Section: Discussionmentioning

confidence: 98%

Construction of SIRT1 gene shRNA lentivirus vector and its effect on the proliferation of breast cancer cells

Yue

Tian

et al. 2020

Cell Mol Biol (Noisy-le-grand)

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Current experiment aimed to investigate the construction of the SIRT1 gene shRNA lentivirus vector and its effect on proliferation of breast cancer cells. Altogether 80 cases of breast cancer tissues and 80 cases of normal adjacent tissues were collected. qPCR was used for detecting SIRT1 expression. Western blot was used to detect the expression of EMT marker protein. The effect of lentivirus infected sh-SIRT1 on the cell biological function of SK-BR-3 and MDA-MB-231 cells was detected. MTT assay was used to detect cell activity, Transwell cell was used to detect cell invasion and migration, and cell apoptosis detected by flow cytometry. Compared with normal tissues adjacent to cancer, the expression of SIRT1 in cancer tissues increased significantly. Compared with human breast epithelial cells (MCF 10A), SIRT1 expression in breast cancer cells (MDA-MB-231, SK-BR-3) increased significantly. The above results showed that SIRT1 was significant greatly expressed in breast cancer. Compared with the sh-Control group, the cell activity, invasion and migration of the sh-SIRT1 group were enhanced, while cell apoptosis was weakened. In the sh-SIRT1 group infected by lentivirus, cell activity, cell invasion and migration decreased, while cell apoptosis increased. Compared with sh-Control, the expression of Î±-catenin, PTEN and E-cadherin in the sh-SIRT1 group in SK-BR-3 and MDA-MB-231 cells was down-regulated, while the expression of N- cadherin, Î²-catenin and Vimentin was up-regulated. Compared with sh-Control, the expression of Î±-catenin, PTEN and E-cadherin in the sh-SIRT1 group infected by lentivirus was up-regulated, while the expression of N- cadherin, Î²-catenin and Vimentin was down-regulated. To sum up, SIRT1 is highly expressed in breast cancer cells. The proliferation of breast cancer cells was inhibited after lentivirus infection with sh-SIRT1.

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“…Mast cells (MCs) have been considered key e ector cells in allergic reactions, and the IgE on them causes an allergic reaction. When patients are exposed to allergens again, allergenspeci c IgE will combine with the allergens, resulting in the rapid release of mediators such as histamine (HIS) causing nasal reactions such as sneezing, nasal discharge, and nasal itching [4]. In addition, the production of cytokines such as HIS, tumor necrosis factor-α (TNF-α), and leukotriene (LT) can promote the in ow of in ammatory cells and further aggravate AR [5].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Saposhnikovia divaricata Aqueous Extracts on the Inflammation and Intestinal Microflora in Allergic Rhinitis Mice

Chen

Wang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Allergic rhinitis (AR) is a type I allergic disease induced by IgE. Traditional Chinese medicine Saposhnikovia divaricata (Turcz.) Schischk (SD) has anti-inflammatory and antiallergic effects. Materials and Methods. AR model mice were constructed with ovalbumin (OVA) sensitization to observe the improving effect of SD treatment on AR by counting the number of sneezing and rubbing the nose, hematoxylin-eosin, periodic acid-Schiff, and toluidine blue stains. In addition, the allergy and inflammatory cytokines levels and inflammatory cells were observed by ELISA and Wright’s-Giemsa stain. The protein levels of the TLR4/TRAF6/NF-κB and IL-6/ROR-γt/STAT3 pathways were measured by immunohistochemistry, quantitative real-time PCR, and western blot. The intestinal microflora abundance in mice was observed by 16S rDNA high-throughput sequencing. Results. SD treatment inhibited the sneezing and rubbing times of the nose, decreased the degree of a dense arrangement of cells and mucosal swelling and the number of goblet and mast cells of nasal lavage fluid, reduced the levels of IgE, histamine, Leukotriene B4, IL-4, IL-5, TNF-α, IL-6, and IL-17, the eosinophils, neutrophils, and lymphocytes number, the LR4, TRAF6, IL-6, ROR-γt, and STAT3 mRNA levels, respectively, while, it increased the IL-2, IL-10, IFN-γ, and TGF-β1 proteins. SD treatment inhibited the NF-κB, p-STAT3, TLR4, TRAF6, and p-IκBα/IκBα proteins. Besides, the effects of OVA and SD treatments were significantly correlated with the abundance of intestinal microflora. The abundances of Cytophagales, Burkholderia, Alteromonadales, Lactococcus, and Clostridiaceae were changed in SD treatment on AR mice. Conclusions. This study provides a possibility that the improvement effect of SD treatment on allergies and inflammation in AR mice may be related to the TLR4/TRAF6/NF-κB and IL-6/ROR-γt/STAT3 pathways and intestinal microflora modulation.

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Effects of lentivirus-mediated CCR3 RNA interference on the function of mast cells of allergic rhinitis in mice

Cited by 13 publications

References 39 publications

Mechanism of lncRNA H19 in Regulating Pulmonary Injury in Hyperoxia-Induced Bronchopulmonary Dysplasia Newborn Mice

Mechanism of lncRNA H19 in Regulating Pulmonary Injury in Hyperoxia-Induced Bronchopulmonary Dysplasia Newborn Mice

Construction of SIRT1 gene shRNA lentivirus vector and its effect on the proliferation of breast cancer cells

The Effects of Saposhnikovia divaricata Aqueous Extracts on the Inflammation and Intestinal Microflora in Allergic Rhinitis Mice

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