Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats

Elgawish, Rania Abdelrahman; Abdelrazek, Heba M. A.

doi:10.1016/j.toxrep.2014.10.010

Cited by 105 publications

(96 citation statements)

References 22 publications

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“…The present study demonstrated the proved results of lead intoxication in lead acetate treated group. The testicular dysfunction was reflected in the semen analysis with remarkable asthenospermia, oligospermia and high percent of abnormalities, which is similar to previous study (Elgawish and Abdelrazek, 2014). Earlier, it has been proved that accumulation of lead in the testis has antispermatogenic effect (Fahim et al, 2013).…”

Section: Discussionsupporting

confidence: 86%

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Nasr¹,

Elmadawy²,

Almadaly³

et al. 2017

AJVS

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Key words:Lead toxicity, apoptosis, male rat, testis, garlic Lead is a highly prevalent heavy metal pollutant in human and animals which has various toxicological effects on the different organs including reproductive system on particular aspect the male testis. Lead (Pb) exposure enhances the generation of Reactive Oxygen Species (ROS) which negatively affect testis structure and functions. The present study aimed to investigate the effect of garlic supplement on daily basis and its prophylactic possibility against lead acetate induced testicular dysfunction. Thirty two adult male rats were divided to four groups, eight animals per each. All animals were maintained under standard laboratory conditions with free access to suitable diet and water supply. The 1 st group was supplied with water and diet and kept without further treatment as a negative control. The 2 nd group was treated by lead acetate( 50mg/L) daily in drinking water as a positive control. The 3 rd group was administered commercial garlic powder preparation only (200 mg/kg b.w.) by stomach tube. The 4 th group was co-treated with lead acetate in drinking water plus garlic in same doses by stomach tube. Then, all animals were scarified after 42 days for clean excision of epididymis and testes. Semen was collected and analyzed for count, motility and viability. Testicular tissues sections were prepared, stained and examined. Oxidative stress markers, SOD and MDA were determined in testicular tissue homogenate. Immunostaining was carried out on sections of testicular tissue for detection of proapoptotic markers caspase-3 and Bax expression. Finally, the level of expression of Bcl-2 and p53 genes was measured in testicular tissue samples as well. The data summary obtained from co treated group signified the protective effect of garlic against potential lead acetate induced undesired testicular apoptosis.

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Section: Discussionsupporting

confidence: 86%

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Nasr¹,

Elmadawy²,

Almadaly³

et al. 2017

AJVS

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“…Lead induced oxidative stress promotes hydrogen peroxide (H 2 O 2 ) generation [4]. Lead acetate (LA) has been shown to decrease male reproductive organ function by causing testicular tissue alterations in the histological patterns of the testis [5]. …”

Section: Introductionmentioning

confidence: 99%

Prophylactic effect of Nigella sativa against lead acetate induced changes in spermiogram, reproductive hormones and gonadal histology of rats

et al. 2016

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Aim:This study was designed to evaluate the prophylactic effect of Nigella sativa (NS) treatment on toxic effects induced by lead acetate (LA) on the reproductive hormones, spermiogram and gonadal histology of rats.Materials and Methods:A total of 20 Sprague-Dawley rats were divided into four groups of five rats each. Group 1 (negative control [NC]) was the NC and was given distilled water, Group 2 served as the positive control (PC) and was administered 10 mg/kg/day of LA per overall survival (OS), Group 3 (T1) was administered 200 mg/kg/daily of NS per OS for a month, and Group 4 (T2) was pretreated with 200 mg/kg/daily of NS per OS for 1 month, followed by 10 mg/kg/daily of LA alone per OS for another. The rats were euthanized at the end of the experimental period for collection of blood and the right caudal epididymis and testis. Serum was used for determination of reproductive hormones by using radioimmunoassay kits. The epididymal segment was cut and homogenized in phosphate-buffered saline, and the homogenate was used for determination of the spermiogram parameters such as sperm concentration, sperm viability, percentage of live sperm, motility and abnormality. Both the epididymis and testis were fixed in 10% buffered formalin for histological processing.Results:The sperm concentration, general, and individual motilities were higher (p<0.05) in the NC and T1 animals, while the T2 had intermediate and the PC had lower (p<0.05) values of each parameter. The percentage sperm viability was higher (p<0.05) in the T1 and lower (p<0.05) in the PC group. However, percentage abnormality was lower in T1, comparable in NC and T2, and higher (p<0.05) in PC. Spermatogenic cell population and epididymal sperm reserve (ESR) were optimal in control and pretreated animals, while the PC had lower spermatids and ESR. The concentration of estradiol (EST) was lower (p<0.05) in the PC and T2, while leuteinizing hormone (LH) concentration was lower (p<0.05) in the PC, and comparable (p>0.05) between control and T2. The concentration of follicle-stimulating hormone (FSH) was comparable (p>0.05) in all groups, while testosterone (TS) hormone concentration was lower (p<0.05) in the PC and higher in the control and T1 groups.Conclusion:This study showed the preventive effects of NS administration against alterations in reproductive hormnes, sperm parameters and gonadal histology caused by LA in rats.

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“…Sub-chronic low Pb exposure produces nephrotoxicity in vivo and in vitro [36], as well as infertility [56] in rodents. To protect against free radical damage, cells activate anti-oxidant enzymes such as SOD1 (Cu/Zn superoxide dismutase).…”

mentioning

confidence: 99%

Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study

Corsetti

Romano

Stacchiotti

et al. 2016

Biol Trace Elem Res

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Lead (Pb) is an environmental oncogenic metal that induces immunotoxicity and anaemia. Emerging evidence has linked Pb toxicity with endoplasmic reticulum-driven apoptosis and autophagy. Glucose-regulated protein of 78 kDa (Grp78 or binding immunoglobulin protein (BiP)), a master endoplasmic reticulum chaperone, drives macrophage activation and regulates protein folding and calcium flux in response to heavy metals.

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Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats

Cited by 105 publications

References 22 publications

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Prophylactic effect of Nigella sativa against lead acetate induced changes in spermiogram, reproductive hormones and gonadal histology of rats

Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study

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