Effects of Laser Irradiation on Pulp Cells Exposed to Bleaching Agents

Abstract

The aim of this study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like cells exposed to a bleaching agent. Mouse dental papilla cell-23 cells were seeded in wells of 24-well plates. Eight groups were established according to the exposure to the bleaching agent and LLLT (0, 4, 10 and 15 J cm À2). Enamel-dentin disks were adapted to artificial pulp chambers, which were individually placed in wells containing Dulbecco's modified Eagle's medium (DMEM). A bleaching agent (35% hydrogen peroxide [BA35%HP]) was applied on enamel (15 min) to obtain the extracts (DMEM + BA35%HP components diffused through enamel/dentin disks). The extracts were applied (1 h) to the cells, and then subjected to LLLT. Cell viability (Methyl tetrazolium assay), alkaline phosphatase (ALP) activity, as well as gene expression of ALP, fibronectin (FN) and type I collagen, were evaluated. The bleaching procedures reduced the cell viability, ALP activity and gene expression of dentin proteins. Laser irradiation did not modulate the cell response; except for FN, as LLLT decreased the gene expression of this protein by the cells exposed to the BA35%HP. It can be concluded that BA35%HP decreased the activities of odontoblasts that were not recovered by the irradiation of the damaged cells with low-level laser parameters tested.

Influence of different types of light on the response of the pulp tissue in dental bleaching: a systematic review

“…These articles were subjected to full-text evaluation that resulted in the exclusion of two articles [40,41]. Finally, 12 articles met the inclusion criteria and were included in this review [8,13,15,19,20,22,29,[42][43][44][45][46].…”

Manifestações Bucais Da Doença Renal Crônica: Uma Revisão De Literatura

“…No entanto, o H2O2 e seus subprodutos podem atingir na câmara pulpar, sendo capaz de promover efeitos nocivos as células pulpares (REIS et al, 2012;HE et al, 2012;MONCADA et al, 2013;BONAFÉ et al, 2013;COSTA et al, 2010;MOOSAVI et al, 2016). De fato, os danos as células pulpares, odontoblastos e fibroblastos, tem sido descrito em estudo in vitro após o contato com agentes branqueadores (DANTAS et al, 2010;LIMA et al, 2013;LIMA et al, 2014). E em estudo in vivo, ocorrendo a sensibilidade dentária pós-clareamento (MOOSAVI et al 2016).…”

“…(2010) indicaram que a irradiação de um laser de 780 nm de baixa potência com densidade de energia de 10 J/cm 2 era capaz de compensar os efeitos citotóxicos de 35% de peróxido de hidrogênio em fibroblastos da polpa humana. Em contraste, Lima et al (2013) e Lima et al (2014), concluíram que tanto o peróxido de hidrogênio como o peróxido de carbamida reduzem a atividade celular dos odontoblastos e seus efeitos prejudiciais não podem ser compensados pela laserterapia com parâmetros definidos.…”

In Vitro differentiation potential of Isolated dental pulp stem cells

“…Odontoblast-like cells can be obtained from human or experimental animals originated from dental or non dental origin (6) . These cells can synthesize proteins typical to that produced from odontoblasts which are associated to dentine matrix protein synthesis and alkaline phosphatase (ALP) activity.…”

“…Odontoblast-like cell has been introduced in dental markets in order to detect cytotoxicity of dental materials products (6) . In vitro study models of odontoblast-like cell lines were developed to mimic the reparative secretory function of odontoblasts in vivo (6)…”