Effects of Laddecreg; on the formation of calcified bone matrix in rat calvariae cells culture

Hofman, Sam; Sidqui, M.; Abensur, D; Valentini, Pascal; Missika, P

doi:10.1016/s0142-9612(97)00082-3

Cited by 28 publications

(30 citation statements)

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“…The present in vivo findings show the presence of needle-like crystals at the surface of implanted particles. These newly formed crystallites were also described with other ABM of bovine origin in vitro and in vivo with Bio-Oss ® [47,48]. These observations are in accordance with investigations carried out on the newly-formed crystal growth mechanisms [49].…”

Section: Discussionsupporting

confidence: 86%

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Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph® and PepGen P-15®

Dahlet¹,

Boukari²,

Collavini³

et al. 2013

JBNB

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The aim of the present study was to investigate an inorganic bovine-derived hydroxyapatite bone substitute (Osteograph ® ) mixed with the same biomaterial coated with a synthetic peptide (P-15) analogue of collagen ). This blend of bone replacement materials was used for sinus floor augmentation. Assessments were carried out by using histology methods, transmission electron microscopy (TEM) and microanalysis (EDX). Ultrastructural and analytical features of the interfaces between the graft material and the peri-biomaterial tissues were evaluated six months after implantation. Our findings clearly show that newly-formed crystallites first develop at the surface of implanted crystals. Histological investigations revealed new bone tissue linking biomaterial particles together. TEM assessments pointed out that lamellar bone was generally separated from the graft material by a layer of woven bone measuring between 1 and 1.5 µm in thickness. Although calcified bone tissue was observed in direct contact with bone filling particles, the presence of mineralized granular material around implanted particles was also noticed. No characteristic periodic striation of mineralized collagen was evident within that mineralized structure. Chemical analyses (TEM-EDX) realized at different locations of newly formed mineralized granular substance along the interface revealed average Ca/P ratios ranging between 1.02 and 1.63. The different, concomitantly occurring, aforementioned structural features of the interfaces strongly suggested that the host responses to the used biomaterial blend resulted from dynamic osseointegration phenomena related to various interfacial mechanisms. Nevertheless, the biological response to the bone graft material appeared clinically and histologically satisfactory.

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Section: Discussionsupporting

confidence: 86%

“…Uniformly adsorbed P-15 cell-ligand on the ABM surface is expected to lead to homogenous formation of new bone tissue. But our findings rather showed focal bone formations as described when using other hydroxyapatites [38,[47][48][49]. The variability of the observed mineral responses, i.e.…”

Section: Discussionsupporting

confidence: 69%

Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph® and PepGen P-15®

Dahlet¹,

Boukari²,

Collavini³

et al. 2013

JBNB

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“…Descriptive studies involving transmission electron microscopy or Von Kossa staining of bone by light microscopy have shown mineralization on collagen sponges, synthetic hydroxapatite (HA) crystals, and Biostile (0.5% bovine type I collagen, 2.5% chondroitin-4-sulphate and HA) with primary human osteoblasts 40 and on Laddec (bovine xenograft) and BioOss (bovine bone mineral) with primary rat calvarial cells. 41 The sequential expression of bone matrix proteins for mineralization in primary rat calvarial osteoblast cultures has been compared on Matrigel (basement membrane matrix), HA, Titanium and tissue culture polystyrene. 42 Primary cultures from rat calvaria have been used extensively in in vitro studies on various substrates 43 due to the welldocumented ability of these cultures to express sequentially bone matrix proteins that subsequently mineralize by nodule formation.…”

Section: Resultsmentioning

confidence: 99%

Functionalized silk-based biomaterials for bone formation

Sofia

McCarthy

Gronowicz

et al. 2000

J. Biomed. Mater. Res.

770

582

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Silks are being reassessed as biomaterial scaffolds due to their unique mechanical properties, opportunities for genetic tailoring of structure and thus function, and recent studies clarifying biocompatibility. We report on the covalent decoration of silk films with integrin recognition sequences (RGD) as well as parathyroid hormone (PTH, 1-34 amino acids) and a modified PTH 1-34 (mPTH) involved in the induction of bone formation. Osteoblast-like cell (Saos-2) responses to the decorated silk films indicate that the proteins serve as suitable bone-inducing matrices. Osteoblast-like cell adhesion was significantly increased on RGD and PTH compared to plastic, mPTH, and the control peptide RAD. At 2 weeks of culture, message levels of alkaline phosphatase were similar on all substrates, but by 4 weeks, alkaline phosphatase mRNA was greatest on RGD. At 2 weeks of culture, alpha 1(I) procollagen mRNA was elevated on silk, RGD, RAD, and PTH, and hardly detectable on mPTH and plastic. However, by 4 weeks RGD demonstrated the highest level compared to the other substrates. Osteocalcin message levels detected by RT-PCR were greatest on RGD at both time points. Calcification was also significantly elevated on RGD compared to the other substrates with an increase in number and size of the mineralized nodules in culture. Thus, RGD covalently decorated silk appears to stimulate osteoblast-based mineralization in vitro.

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“…poly(P) stimulates bFGF-induced cell proliferation and vascularization, and these effects resulted in a significantly higher BIC in the Poly(P)/IP-CHA group than the IP-CHA group. A bovine-derived anorganic bone matrix such a Bio-Oss ® has been reported superior bone formation and provides scaffold for osteoblasts as an alternative to autologous bone [20][21][22] .…”

Section: Discussionmentioning

confidence: 99%

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

et al. 2014

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Inorganic polyphosphate (poly(P)) is recognized as a therapeutic agent that promotes fibroblast growth factor and enhances osteogenic differentiation, and in vivo, when adsorbed onto interconnected porous calcium hydroxyapatite (IP-CHA) enhances bone regeneration. The present study focused on the effect of poly(P) adsorbed onto IP-CHA granules (Poly(P)/IP-CHA) in guided bone regeneration (GBR). Dental implants were placed into the edentulous mandibular areas of five Beagle-Labrador hybrid dogs with screw expose on the buccal side, and then bone defects were filled Poly(P)/IP-CHA (test) or IP-CHA (control). After 12 weeks, histological evaluation and histomorphometrical analysis were performed. Newly-bone formation around exposed implant screw was clearly detected in the test-group. The ratio for regenerated bone height in the test group versus the control-group was 85.6±20.2 and 62.6±23.8, respectively, with no significant difference, while, that for bone implant contact was significantly higher (67.9±11.8 and 48.8±14.1, respectively). These findings indicate that Poly(P)/IP-CHA enhances bone regeneration in GBR.

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Effects of Laddecreg; on the formation of calcified bone matrix in rat calvariae cells culture

Cited by 28 publications

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Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph<sup>®</sup> and PepGen P-15<sup>®</sup>

Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph<sup>®</sup> and PepGen P-15<sup>®</sup>

Functionalized silk-based biomaterials for bone formation

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

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Effects of Laddecreg; on the formation of calcified bone matrix in rat calvariae cells culture

Cited by 28 publications

References 0 publications

Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph&lt;sup&gt;&#174;&lt;/sup&gt; and PepGen P-15&lt;sup&gt;&#174;&lt;/sup&gt;

Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph&lt;sup&gt;&#174;&lt;/sup&gt; and PepGen P-15&lt;sup&gt;&#174;&lt;/sup&gt;

Functionalized silk-based biomaterials for bone formation

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

Contact Info

Product

Resources

About

Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph<sup>®</sup> and PepGen P-15<sup>®</sup>

Ultrastructure and Analytical Features of the Sinus Floor Augmentation with Osteograph<sup>®</sup> and PepGen P-15<sup>®</sup>