Effects of Lactobacillus salivarius, Lactobacillus reuteri, and Pediococcus acidilactici on the nematode Caenorhabditis elegans include possible antitumor activity

Abstract

This study examined the effects of three lactic acid bacteria (LAB) strains on the nematode Caenorhabditis elegans. Lactobacillus salivarius, Lactobacillus reuteri, and Pediococcus acidilactici were found to inhibit the development and growth of the worm. Compared to Escherichia coli used as the control, L. reuteri and P. acidilactici reduced the lifespan of wild-type and short-lived daf-16 worms. On the contrary, L. salivarius extended the lifespan of daf-16 worms when used live, but reduced it as UV-killed bacteria. The three LAB induced the expression of genes involved in pathogen response and inhibited the growth of tumor-like germ cells, without affecting DAF16 localization or increasing corpse cells. Our results suggest the possible use of C. elegans as a model for studying the antitumor attributes of LAB. The negative effects of these LAB strains on the nematode also indicate their potential use against parasitic nematodes.

Commensals, probiotics and pathogens in theCaenorhabditis elegansmodel

“…Studies in C. elegans have shown a mildly beneficial effect in terms of both life and health span for nematodes grown on some strains of Lactobacillus (Ikeda et al, 2007;Lee et al, 2011), although the results are highly strain-dependent and are also affected by factors such as proliferative capability of the bacteria. Some lactic acid bacteria have also been shown to have a detrimental effect on C. elegans lifespan (Fasseas et al, 2013).…”

“…2A). Several studies Fasseas et al, 2013;Komura et al, 2013;MacNeil et al, 2013) have ruled out the involvement of DAF-16 in probiotic-mediated lifespan extension, indicating that there are multiple independent pathways affecting C. elegans longevity.…”

Analysis of the transcriptome of Hirschmanniella oryzae to explore potential survival strategies and host–nematode interactions

“…These last three are probably involved in protection against bacterial pathogens (Evans et al, 2008;Zavalova et al, 2006). It has been shown that TLPs are up-regulated on bacterial infection in C. elegans (Fasseas et al, 2012). Seven TLPs were found in the full dataset, four of which contained a predicted secretion signal.…”

Host-Microbe Interactions inCaenorhabditis elegans

“…Three lactic bacteria strains, Lactobacillus salivarius , Lactobacillus reuteri , and Pediococcus acidilactici , have been found to hinder the worm's development and growth, obstruct the germ cells growth, and induce the gene expressions involved in pathogen response [28]. …”