Effects of l-aspartate on cellular Na+ K+ and Cl− activities in retzius nerve cells of the leech

Cemerikic, Dusan; Nedeljkov, Vladimir; Beleslin, B

doi:10.1016/0300-9629(88)91140-1

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…In the case of a significant contribution of monovalent cations to the 5-HT-mediated response, the reversal potential for the combination InCl/Ringer's should be shifted to positive potentials relative to InCl/ExCl. Because the measured shift did not exceed the calculated shift in EC,, these results confirm the finding that Retzius cells respond to 5-HT application predominantly by Cl currents (Walker and Smith, 1973;Cemerikic et al, 1985). With Cll in external and internal solutions, currentvoltage relationships were approximately linear around the reversal potential, and slope conductances could be evaluated.…”

Section: Resultssupporting

confidence: 79%

“…Our experiments show that the first 5-HT-activated currents in developing Retzius cells are carried by Cl ions, which also dominate the 5-HT response of adult cells (Walker and Smith, 1973;Cemerikic et al, 1985;Drapeau et al, 1989). The first responses to 5-HT were recorded at E 10.…”

Section: Discussionmentioning

confidence: 64%

“…Adult cells. Retzius cells in vivo as well as in vitro respond to 5-HT application with a hyperpolarizing Cl-current (Walker and Smith, 1973;CemerikiC et al, 1985). In addition, there is evidence for a second 5-HT receptor, mediating an initial small depolarization (Erwin and Thies, 1975;) by flow of monovalent cations (Drapeau et al, 1989).…”

Section: Resultsmentioning

confidence: 99%

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Development of serotonin-induced ion currents in identified embryonic Retzius cells from the medicinal leech (Hirudo medicinalis)

Leßmann¹,

Dietzel²

1991

J. Neurosci.

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Retzius cells and the cutaneous baroreceptive P-cells of adult medicinal leeches (Hirudo medicinalis) respond to 5-HT by Cl- as well as by monovalent cation conductances (Drapeau et al., 1989). However, chemical synaptic connections between Retzius cells (Liu and Nicholls, 1989) as well as Retzius cells and P-cells in culture (Drapeau and Sanchez-Armass, 1988) are mediated by postsynaptic Cl- currents in response to 5-HT release from the presynaptic cell. It was the aim of the present study to find out whether the response, which has been shown to be involved in synaptic transmission or whether the cation current, which has so far only been observed upon extrasynaptic 5-HT application, dominates during embryogenesis. Currents induced by transmitter application with a fast perfusion system were measured with a single-electrode voltage clamp and patch pipettes in whole-cell configuration. Control experiments, performed on Retzius cells from adult leeches after 1 d in culture, showed Cl- currents in response to 5-HT application in all cells investigated. Ninety percent of the cells tested showed additional 5-HT-elicited Na+, K+, or both currents. However, the conductance of cation currents amounted only to 10% of the Cl- currents. Embryonic Retzius cells were identified in dissociated cultures by their violet-blue fluorescence acquired by preincubation of intact ganglia or embryos in culture medium containing the autofluorescent 5-HT analog 5,7-dihydroxytryptamine. The first responses to 5-HT were measured at embryonic day 10 (E10), after voltage-activated Ca2+ and Na+ currents had already developed. 5-HT application induced exclusively Cl- currents until E16. This suggests that the receptor-channel complex, which also mediates synaptic responses, is expressed first in measurable quantities. Hence, there is currently no indication that extrasynaptic receptors dominate in immature cells in this preparation.

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Section: Resultssupporting

confidence: 79%

Section: Discussionmentioning

confidence: 64%

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Development of serotonin-induced ion currents in identified embryonic Retzius cells from the medicinal leech (Hirudo medicinalis)

Leßmann¹,

Dietzel²

1991

J. Neurosci.

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“…They postulated that the effect of L-asp on membrane potetntial (E m ) is carried by these ions. This has subsequently been proven by CEMERIKIC et al (1988) through usage of ion-selective microelectrodes, who concluded that, similarly to Lglu, the efflux of potassium is a passive and sodium dependent event.…”

mentioning

confidence: 91%

Comparison of Effects of Endogenous and Exogenous Excitatory Amino Acids on Retzius Nerve Cells of the Leech

Lopičić¹,

Nedeljkov²,

Cemerikic³

et al. 2008

folia biol (krakow)

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In this paper we examine the effects of L-aspartate, L-glutamate, and $-N-oxalylamino-L-alanine (Lathyrus toxin) on Retzius nerve cells of the leech Haemopis sanguisuga. The goal was to compare the electrophysiological effects of endogenous vs. exogenous amino acids, known as potent neurotoxins, through the mechanism of excitotoxicity. We used classical intracellular recordings on Retzius nerve cells in isolated ganglia of the leech, and plotted dose-response curves to compare potencies. Our results show that Lathyrus toxin is more than 200 times more potent in depolarizing the membrane potential on our model than L-aspartate and L-glutamate, which are approximately equipotent.

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Effects of acid Ca2+ ringer on passive electrical properties and intracellular ion activities in leech retzius neuron

Cemerikic¹,

Nedeljkov²,

Galogaza³

et al. 1992

Comparative Biochemistry and Physiology Part A: Physiology

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Effects of l-aspartate on cellular Na+ K+ and Cl− activities in retzius nerve cells of the leech

Cited by 5 publications

References 28 publications

Development of serotonin-induced ion currents in identified embryonic Retzius cells from the medicinal leech (Hirudo medicinalis)

Development of serotonin-induced ion currents in identified embryonic Retzius cells from the medicinal leech (Hirudo medicinalis)

Comparison of Effects of Endogenous and Exogenous Excitatory Amino Acids on Retzius Nerve Cells of the Leech

Effects of acid Ca2+ ringer on passive electrical properties and intracellular ion activities in leech retzius neuron

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