Effects of K<sup>+</sup> diffusion potentials on duroquinol‐cytochrome <i>c</i> reductase activity catalyzed by complex III incorporated into liposomes

Wielburski, Antek; Nelson, Bruce R.

doi:10.1016/0014-5793(78)80807-2

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…III with phosphatidylcholine are impermeable to K+ Lipid/protein ratio (Wielburski & Nelson, 1978) or protons, low Fig. 1.…”

Section: Discussionmentioning

confidence: 97%

“…(Table 1). Vesicles formed with phosphatidylcholine were relatively impermeable to univalent cations, as indicated by their ability to form a stable membrane potential from K+ diffusion gradients (Wielburski & Nelson, 1978). Thus the high electron-transfer rates obtained with phosphatidylcholine vesicles in the absence of carbonylcyanide p-trifluoromethoxyphenylhydrazone suggests that Complex III is not properly inserted in the membrane.…”

Section: Reconstitution Of Respiratory Control With Individualphosphomentioning

confidence: 99%

“…Ubiquinone-cytochrome c oxidoreductase (Complex III) purified from mitochondria has previously been incorporated into phospholipid vesicles that exhibit controlled electron transport (Leung & Hinkle, 1975; Guerrieri & Nelson, 1975;Eytan et al, 1977;Wielburski & Nelson, 1978). Such functional reconstitution has been obtained with widely varying lipid compositions.…”

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confidence: 99%

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Phospholipid requirements for the reconstitution of complex-III vesicles exhibiting controlled electron transport

Nelson¹,

Fleischer²

1981

Biochemical Journal

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Phospholipid requirements for the reconstitution of Complex-III vesicles exhibiting respiratory control (electron-transport control) were studied. Vesicles prepared from pure phosphatidylethanolamine gave maximal control ratios. Phosphatidylcholine alone did not support respiratory control, although these vesicles were capable of maintaining stable K+-diffusion gradients. Apparently Complex III cannot insert into a bilayer of phosphatidylcholine. Formation of mixed phosphatidylcholine/phosphatidylethanolamine (6:1, w/w) vesicles was sufficient, however, to allow Complex-III insertion and to restore respiratory control. Mixtures of acidic phospholipids with either phosphatidylethanolamine or phosphatidylcholine did not improve respiratory control over that obtained with pure phosphatidylethanolamine. Phosphatidylethanolamine from bovine heart mitochondria, soya beans or Escherichia coli was equally effective in reconstituting respiratory control, suggesting that the specificity is referable to the head group and not to the fatty-acid moiety.

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“…III with phosphatidylcholine are impermeable to K+ Lipid/protein ratio (Wielburski & Nelson, 1978) or protons, low Fig. 1.…”

Section: Discussionmentioning

confidence: 97%

Section: Reconstitution Of Respiratory Control With Individualphosphomentioning

confidence: 99%

mentioning

confidence: 99%

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Phospholipid requirements for the reconstitution of complex-III vesicles exhibiting controlled electron transport

Nelson¹,

Fleischer²

1981

Biochemical Journal

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Light-activated proton-motive force generation in lipid vesicles containing cytochrome b-c1 complex and bacterial reaction centres

Rich

Heathcote

1983

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Reconstitution of cytochrome bc₁ complex into lipid vesicles and the restoration of uncoupler sensitivity

Rich¹,

Clarke²

1982

FEBS Letters

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Beef heart mitochondrial bc 1 complex (ubiquinone-cytochrome c oxidoreductase) has been assayed by its ability to catalyse the reaction of duroquinol reduction of ferricytochrome c. When the isolated complex is reincorporated into lipid vesicles, the enzyme-catalysed electron transfer rate becomes uncoupler-sensitive. Initial experiments suggest that a protonmotive force is generated across the vesicles when electron transfer is initiated. Both A* and ApH components of this protonmotive force then influence an internal rate constant of the bcl complex. Mitochondrial bc complexLipid vesicle

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Effects of K⁺ diffusion potentials on duroquinol‐cytochrome c reductase activity catalyzed by complex III incorporated into liposomes

Cited by 9 publications

References 16 publications

Phospholipid requirements for the reconstitution of complex-III vesicles exhibiting controlled electron transport

Phospholipid requirements for the reconstitution of complex-III vesicles exhibiting controlled electron transport

Light-activated proton-motive force generation in lipid vesicles containing cytochrome b-c1 complex and bacterial reaction centres

Reconstitution of cytochrome bc₁ complex into lipid vesicles and the restoration of uncoupler sensitivity

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Effects of K+ diffusion potentials on duroquinol‐cytochrome c reductase activity catalyzed by complex III incorporated into liposomes

Cited by 9 publications

References 16 publications

Phospholipid requirements for the reconstitution of complex-III vesicles exhibiting controlled electron transport

Phospholipid requirements for the reconstitution of complex-III vesicles exhibiting controlled electron transport

Light-activated proton-motive force generation in lipid vesicles containing cytochrome b-c1 complex and bacterial reaction centres

Reconstitution of cytochrome bc1 complex into lipid vesicles and the restoration of uncoupler sensitivity

Contact Info

Product

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Effects of K⁺ diffusion potentials on duroquinol‐cytochrome c reductase activity catalyzed by complex III incorporated into liposomes

Reconstitution of cytochrome bc₁ complex into lipid vesicles and the restoration of uncoupler sensitivity