Effects of Jaoga‐Yukmiwon^®, a Korean herbal medicine, on chondrocyte proliferation and longitudinal bone growth in adolescent male rats

Abstract

Externally visible growth of the body is the result of proliferation of chondrocytes and longitudinal bone growth. The effects of the Korean herbal medicine, Jaoga-Yukmiwon((R)), on the growth of adolescent rats were investigated in the present study. The proliferation ratio of chondrocytes was calculated from 5-bromo-2'-deoxyuridine incorporation during DNA synthesis. The 5-bromo-2'-deoxyuridine incorporation ratio of the control group was 21.1 +/- 3.5%, and Jaoga-Yukmiwon((R)) administration markedly increased the ratio to 34.5 +/- 4.6% (p < 0.05). The bone formation rate of longitudinal bone was estimated by labelling with tetracycline, which binds to newly formed bone. The bone formation rate in the tibia of Jaoga-Yukmiwon((R))-administered rats was increased to 405.1 +/- 4.3 micro m (p < 0.05) from 292.2 +/- 11.8 micrometer (control value). The height of the growth plate in the administrated rats was increased to 614.6 +/- 10.0 micro m (p < 0.05) from 552.3 +/- 17.1 micro m. The bone morphogenetic protein-2 immunostaining in the growth plate was also increased. These results suggest that Jaoga-Yukmiwon((R)) may promote longitudinal bone growth during the developmental period.

Yeast hydrolysate induces longitudinal bone growth and growth hormone release in rats

“…The growth plate consists of four distinct histological zones, beginning with the resting zone and extending through the proliferative and hypertrophic zones (Leem et al, 2003). Since the growth plate height is correlated to the rate of body growth (Bass et al, 1999), the growth plate heights of the rats were measured.…”

The Herbal Formula HT042 Induces Longitudinal Bone Growth in Adolescent Female Rats

“…A. membranaceus was reported to accelerate the proliferation and promotion of bone marrow stromal cells 2 and to induce GH in pituitary cell culture. 3 E. senticosus was reported to promote the longitudinal bone growth in adolescent male rats 4 and to increase bone mineral density of the mouse tibia. 5 Bone growth is a result of endochondral cell proliferation in growth plates and conversion of chondrocytes into new bone.…”

“…We measured the bone growth of tibia and BrdU-positive cells on chondrocytes in the growth plate zone. 4 Additionally, the body weight, the nosetail (N-T) length, and the nose-anus (N-A) length for 4 weeks were measured by microknemometry. 8 …”

Effect of Copper on the Expression of TGF-β in Incubated Chondrocytes of Newborn Pigs

“…In the recent years, because of regulation of cell growth, differentiation, and immune function, TGF-β in the treatment of wound healing, promoting repair of cartilage and bone, has potential application concern [21]. The correlation research indicated that TGF-β is a regulatory protein, which is widely expressed in bone tissues, especially in chondrocytes, and in platelets [22][23][24]. Stimulation of chondrocytes in vitro with TGF-β can produce type-I collagen, inducing differentiation from mesenchymal cells to chondrocytes and formation of cartilage tissue [25,26].…”