Effects of ischemia and omeprazole preconditioning on functional recovery of isolated rat heart

Abstract

ObjectiveThe aim of this study was to compare protective effects of ischemic and potential protective effects of pharmacological preconditioning with omeprazole on isolated rat heart subjected to ischemia/reperfusion.MethodsThe hearts of male Wistar albino rats were excised and perfused on a Langendorff apparatus. In control group (CG) after stabilization period, hearts were subjected to global ischemia (perfusion was totally stopped) for 20 minutes and 30 minutes of reperfusion. Hearts of group II (IPC) were submitted to ischemic preconditioning lasting 5 minutes before 20 minutes of ischemia and 30 minutes of reperfusion. In third group (OPC) hearts first underwent preconditioning lasting 5 minutes with 100μM omeprazole, and then submitted 20 minutes of ischemia and 30 minutes of reperfusion.ResultsAdministration of omeprazole before ischemia induction had protective effect on myocardium function recovery especially regarding to values of systolic left ventricular pressure and dp/dt max. Also our findings are that values of coronary flow did not change between OPC and IPC groups in last point of reperfusion.ConclusionBased on our results it seems that ischemic preconditioning could be used as first window of protection after ischemic injury especially because all investigated parameters showed continuous trend of recovery of myocardial function. On the other hand, preconditioning with omeprazole induced sudden trend of recovery with positive myocardium protection, although less effective than results obtained with ischemic preconditioning not withstand, we must consider that omeprazole may be used in many clinical circumstances where direct coronary clamping for ischemic preconditioning is not possible.

Effects of Ischemic and Proton Pump Inhibitors Preconditioning on Oxidative Stress of Isolated Rat Heart

“…In a study with omeprazole is observed that the drug significantly contribute to a recovery in isolated rat heart after injury caused by myocardial ischemia and reperfusion. Similar conclusions are performed from a survey conducted with pantoprazole on isolated rat heart and same findings we have from our previous work (7)(8)(9).…”

Gastroesophageal reflux disease and atrial fibrillation: comorbidity or overlap syndrome? View of gastroenterologist

“…in vitro изучали влияние введения ИПП в кровоток крыс на степень повреждения миокарда до и после индукции ишемии. Было выявлено, что введение омепразола до индукции ишемии оказывало защитное действие на восстановительную функцию миокарда; это позволило сделать заключение, что ИПП могут иметь антиаритмический и кардиопротективный эффекты [40].…”

Atrial fibrillation and gastrooesophageal reflux disease: association mechanisms, treatment approaches

“…Участие К+/Н+-АТФазы в регуляции функции серд ца было подтверждено в лабораторных условиях: желудочные изоформы К+/Н+-АТФазы и рецепторы для связывания ИПП присут ствуют в клетках сердца млекопитающих. Ученые пришли к заключению, что ИПП могут иметь анти аритмический и кардиопротективный эффекты [35].…”