Effects of ipriflavone administration on bone mass and metabolism in ovariectomized women

Gambacciani, Marco; Spinetti, A.; Cappagli, Barbara; Taponeco, Fabio; Felipetto, R.; Parrini, D; Cappelli, N; Fioretti, P.

doi:10.1007/bf03348848

Cited by 55 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in bone resorption markers preceding the increase in bone formation markers is consistent with the results of earlier cross-sectional studies (25-28) and longitudinal studies with biochemical markers other than PICP and ICTP (15,16), and this finding supports the general concept that the loss of skeletal balance shortly after acute estrogen deficiency is due to the prevalence of bone resorption over new bone deposition (29). Our results, however, clearly demonstrated a gradual decline in bone resorption after 6 months P0.…”

Section: Changes In Lumbar Bmdsupporting

confidence: 89%

“…Furthermore, changes in bone metabolism after oophorectomy have been reported in previous studies by using serum BGP levels and/or bone alkaline phosphatase activity as indices of bone formation and urinary hydroxyproline excretion and/or plasma tartrate-resistant acid phosphatase as indices of bone resorption (15,16). The details of changes in serum PICP and ICTP in a longitudinal study, however, have never been reported.…”

mentioning

confidence: 92%

See 1 more Smart Citation

Changes in Serum Levels of Type I Collagen-Related Proteins after Surgically Induced Menopause and Correlations with Bone Loss in the Lumbar Spine.

1999

View full text Add to dashboard Cite

Abstract.The purpose of this prospective study was to characterize the changes in serum levels of two proteins produced during the synthesis and degradation of type I collagen, i.e., the carboxyterminal propeptide of type I procollagen (PICP) and the pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP), respectively, after oophorectomy, and to assess the degree of correlation between changes in the serum values of these proteins and changes in bone mineral density (BMD) of the lumbar spine. Serum levels of PICP, ICTP and bone gla protein (BGP) were determined in 18 women before oophorectomy (baseline) and at 7 days, and 1, 2, 3, 6, 9 and 12 months post-oophorectomy (PO). The BMD of the lumbar spine was measured at baseline, and at 6 months and 12 months P0. ICTP had increased significantly at 7 days PO and peaked between 1 and 3 months P0. PICP and BGP had increased significantly at 2 months PO and remained at high levels thereafter.The percent changes in lumbar BMD from baseline values (% CFB) at 6 months and at 12 months PO were significantly correlated with % CFB in ICTP, but not with % CFB in PICP or BGP. Accordingly, bone resorption is a main determinant of bone mineral loss after oophorectomy and the change in recently-developed bone resorption markers, such as ICTP, is of clinical utility in predicting a degree of subsequent bone loss after surgical menopause. Key words:Oophorectomy, PICP, ICTP, Bone mineral density, Correlation (Endocrine Journal 46: 337-343, 1999) THE serum carboxyterminal propeptide of type I procollagen (PICP) and the pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP) reflect the rate of formation and degradation of type I collagen, respectively (1-3), and their performance as bone biochemical markers was found to be promising in studies using a method of bone histomorphometry (4) or calcium kinetics (5). Simultaneous determination of bone formation and resorption can therefore be made by measuring PICP and ICTP in serum specimens from the examinees.

show abstract

Section: Changes In Lumbar Bmdsupporting

confidence: 89%

mentioning

confidence: 92%

Changes in Serum Levels of Type I Collagen-Related Proteins after Surgically Induced Menopause and Correlations with Bone Loss in the Lumbar Spine.

1999

View full text Add to dashboard Cite

show abstract

“…Ipriflavone per se has been shown to exert beneficial effects on the osteopenia induced in women by either iatrogenic [2] or physiological estrogen deficiency [1,3]. Nevertheless, it is well documented that dietary flavonoids alter the hormonal status of premenopausal women [12].…”

Section: Discussionmentioning

confidence: 99%

“…First clinical trials indicate that the compound exerts a beneficial effect in osteopenic diseases [1][2][3]. It is not clear yet whether ipriflavone acts primarily by inhibiting bone resorption or by stimulating bone formation.…”

mentioning

confidence: 99%

Ipriflavone inhibits bone resorption in intact and ovariectomized rats

1997

View full text Add to dashboard Cite

Abstract. The aim of this study was to investigate the possible inhibitory effect of ipriflavone on bone resorption in rats. For this purpose, 10-week-old, intact and ovariectomized (OVX) rats, prelabeled from birth with [3 H]-tetracycline, were used. Bone resorption was monitored by measuring the urinary excretion of [ 3 H]. The animals were fed a purified diet devoid of naturally occurring flavonoids. In the intact rats, the daily meal was given either as a single portion or divided into four portions, a procedure known to lead by itself to a decrease in bone resorption. Ipriflavone, given 7 days after OVX at the dose of 400 mg/kg B.W. daily mixed with the food, led within 2-3 days to a significant decrease in bone resorption equivalent to that of 27.2 p g/kg S.C. of 1713-estradiol. The inhibition was sustained for the length of the experiment, up to 21 days. Ipriflavone given 7 days before OVX prevented the increase in bone resorption induced by castration, the effect being dose-dependent between 50 and 400 mg/kg B.W. In contrast to 1713-estradiol, a 5-week treatment with ipriflavone failed to prevent the OVX-induced uterine atrophy. Significant inhibition of bone resorption was also seen in intact animals, provided they rapidly ingested the daily meal. Actually, the decrease in bone resorption induced by portioning the daily food masked the inhibitory effect of ipriflavone in intact animals. In conclusion, ipriflavone can decrease bone resorption in both intact and OVX animals given a purified diet as a single daily meal. In the OVX model, ipriflavone mimics the osteoprotective effect of estrogen. However, the lack of a uterotropic effect suggests that the compound can discriminate between bone and reproductive tissues.Key words: Ipriflavone -Bone resorption -Ovariectomized rats.Ipriflavone is a synthetic derivative of a natural isoflavone. First clinical trials indicate that the compound exerts a beneficial effect in osteopenic diseases [1][2][3]. It is not clear yet whether ipriflavone acts primarily by inhibiting bone resorption or by stimulating bone formation. Inhibition of osteoclast recruitment in both in vitro [4,5] and in vivo [6], and induction of markers of the osteoblast phenotype in vitro [7,8] have been demonstrated. However, in the rat model of osteopenia following ovariectomy [9], ipriflavone did not reduce bone resorption when administered alone, but it did enhance the therapeutic effects of estrogen. Confounding factors may explain the lack of the protective effect of ipriflavone per se on bone loss due to estrogen withdrawal in animals. Therefore, we investigated in vivo the modalities by which an intrinsic inhibitory activity of ipriflavone on bone resorption can be disclosed.For measuring bone resorption, we used a technique based on long-term prelabeling with [ 3 H]-tetracycline and the subsequent determination of urinary excretion of the tracer (Fig. 1). This technique allows monitoring of bone resorption, daily or at shorter intervals, over extended periods of time [10].According to t...

show abstract

“…Several therapeutic agents, such as bisphosphonate, calcium, vitamin D3, vitamin K, estrogen, ipriflavon, and calcitonin, have been used for the treatment of osteoporosis ( Black et al, 1996;Gambacciani et al, 1993;Iketani & Kiriike, 2003;Recker et al, 1996;Rico, Hernandez, Revilla, & GomezCastresana, 1992). Although estrogen deficiency has been considered to be an important etiologic contributor to bone loss in AN, the effect of estrogen treatment for patients with AN is currently inconclusive ( Klibanski, Biller, Schoenfeld, Herzog, & Saxe, 1995;Kreipe, Hicks, Rosier, & Puzas, 1993;Maugars et al, 1996;Seeman, Szmukler, Formica, Tsalamandris, & Mestrovic, 1992).…”

Section: Resultsmentioning

confidence: 99%

The effects of bone therapy on tibial bone loss in young women with anorexia nervosa

Nakahara

Nagai

Tanaka

et al. 2006

Int. J. Eat. Disord.

View full text Add to dashboard Cite

Effects of ipriflavone administration on bone mass and metabolism in ovariectomized women

Cited by 55 publications

References 11 publications

Changes in Serum Levels of Type I Collagen-Related Proteins after Surgically Induced Menopause and Correlations with Bone Loss in the Lumbar Spine.

Changes in Serum Levels of Type I Collagen-Related Proteins after Surgically Induced Menopause and Correlations with Bone Loss in the Lumbar Spine.

Ipriflavone inhibits bone resorption in intact and ovariectomized rats

The effects of bone therapy on tibial bone loss in young women with anorexia nervosa

Contact Info

Product

Resources

About