Effects of ions on the motility of fresh and demembranate spermatozoa of common carp (Cyprinus carpio) and paddlefish (Polyodon spathula)

Linhart, Otomar; Cosson, Jacky; Mims, Steven D.; Rodina, Marek; Gela, David; Shelton, William L.

doi:10.1023/b:fish.0000030528.03098.6d

Cited by 30 publications

(29 citation statements)

References 4 publications

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“…Among the above mentioned factors, K + concentration is a key factor in combination with osmotic pressure that control sperm motility and allow it to be initiated in salmonids [49,84], sturgeons and paddlefish [53,62,67,69,70,71,81,94,95]. It has been known since 1938 that millimolar level of extracellular K ion concentration ([K + ] o ) in the seminal tract is primarily responsible for keeping trout sperm inactive [96].…”

Section: Effect Of Ionsmentioning

confidence: 99%

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Tale of Fish Sperm and Factors Affecting Sperm Motility: A Review

Islam¹,

Akhter²

2012

ALS

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Motility is an important function of the male gamete, which allows sperm to actively reach and penetrate the female gamete in organisms with internal and external fertilization. Sexual activity of some fish is generally seasonal and fertilization is external. Sperm, once differentiated in the gonad, remain there completely quiescent until they are released into the external medium, which is either freshwater or sea water. Various parameters such as ion concentrations (K + , Na + , Ca 2+ ), osmotic pressure, pH, and temperature affect motility. In the present paper, we review the roles of these factors on sperm motility in the teleosts. Studying the effects of these factors on teleost sperm can help establish good activation and/or immobilizing media for improving either artificial fertilization or cryopreservation.

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Section: Effect Of Ionsmentioning

confidence: 99%

“…The potent effects of K + ion were investigated in demembranated flagella; axonemal motility was found to be directly controlled by the ion concentration [69,70,71]. It is also clear that the K + concentrations in diluents used for cryopreservation strongly influence the potential motility of carp sperm [100].…”

Section: Effect Of Ionsmentioning

confidence: 99%

Tale of Fish Sperm and Factors Affecting Sperm Motility: A Review

Islam¹,

Akhter²

2012

ALS

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“…This index record a mean value of 25.99 ± 0.38 (Ic1) and 15.33 ± 0.22 (Ic2), thus, the fish have a high percentage of meat. The fact that carnosity index values fluctuate around 20%, indicates that the studied specimens have proper ), which presents a significant importance in initiating sperm mobility (Linhart et al, 2003a ) are much more effective in suppressing the K + inhibitory effect on sperm motility than Na + . The effect of inhibiting K + mobility can be counteracted by increasing the concentration of Ca 2+ from outside (Billiard and Cosson, 1992).…”

Section: Resultsmentioning

confidence: 92%

The Effect of Saline Solution Used as an Activating Factor of the Semen in Brown Trout, Salmo Trutta Fario (Linnaeus 1758)

Uiuiu¹,

Cocan²,

Laţiu³

et al. 2017

BUASVMCN-ASB

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In this research we analysed the phenotypic characterization of Salmo trutta fario species and we studied the effect of the saline solution used as an activator for seminal material. The spermatozoa mobility ratio in our study ranged from 60% to 95%, with an average of 74% when using water and 84.5% when a saline solution (NaCl) of 7‰ was used. The average mobility time ranges between 58.7 seconds (water) and 99 seconds (saline solution). Biochemical analyses of seminal plasma were performed and parameters such as ions concentration (Na + , K + , Ca 2+, Mg 2+ , Cl -), protein profile (BUN, TP), lipid profile (TG, Chol) and enzyme profile (ALP) were followed. As a result of our research on semen in brown trout, we noticed that saline solution (7‰) compared to water when used as an activator of seminal material, is more effective in activating spermatozoa.

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“…In immotile samples (n=10 males), the motility of sperm was activated by using 50 mM NaCl (pH 8.0), and for the activating agent, this triggered sperm mobility was used and light microscobe was used for estimating it subjectively. (Linhart et al, 2003). In the subsequent analyses, it is required to use the samples which has only samples at least 80% motile sperm.…”

Section: Spermatozoa Motility Osmolality and Concentrationmentioning

confidence: 99%

The Development of Optimal Cryopreservation Media For Longspine Scraper (Capoeta trutta) Sperm

Şahinöz

Doğu

Aral

2018

Turkish JAF Sci.Tech.

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This study is performed to determine some of sperm quality after applying freezing / thawing process. Thus, the aim of this study is to examine different cryprotective agents with additives in terms of their effects at different pH on the cryopreservation process of longspine scraper (Capoeta trutta). The present study, twelve media were prepared by mixing three different cryoprotectants (dimethyl sulfoxide (DMSO, (CH3)2SO); methanol (CH3OH); methyl glycol (MG, CH3O (CH2)2OH)) with an extenders (glucose) at four different pH (7.2, 7.6, 8.0 and 8.4) for longspine scraper semen. Considering the findings from the examination (The motility rate after thawing process and duration of motility obtained in DMSO as 81% and 20 min, in methanol as 73% and 12 min, in methyl glycol as 60% and 15 min.), we can conclude that the DMSO is the best freezing media in order to create new essays in cryopreservation for sperm of Capoeta trutta in the future.

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Effects of ions on the motility of fresh and demembranate spermatozoa of common carp (Cyprinus carpio) and paddlefish (Polyodon spathula)

Cited by 30 publications

References 4 publications

Tale of Fish Sperm and Factors Affecting Sperm Motility: A Review

Tale of Fish Sperm and Factors Affecting Sperm Motility: A Review

The Effect of Saline Solution Used as an Activating Factor of the Semen in Brown Trout, Salmo Trutta Fario (Linnaeus 1758)

The Development of Optimal Cryopreservation Media For Longspine Scraper (Capoeta trutta) Sperm

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