Effects of ions and drugs on cell membrane activity and tension in the postpartum rat myometrium

Csapó, István; Kuriyama, Hirosi

doi:10.1113/jphysiol.1963.sp007081

Cited by 38 publications

(17 citation statements)

References 25 publications

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“…On the other hand the Cl-makes an important contribution to the membrane conductance of rat myometrium. This confirms the findings of Csapo & Kuriyama (1963) and Abe (loc. cit.…”

Section: Discussionsupporting

confidence: 81%

Ionic fluxes in rat uterine smooth muscle.

Hamon¹,

Papadimitriou²,

Worcel³

1976

The Journal of Physiology

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SUMMARY1. The K+, Na+ and C1-fluxes from the oestrogen-stimulated rat uterine smooth muscle were measured using radioactive tracers.2. The cellular compartment contained a concentration of K+ of 173-6 mm which exchanged at a rate of 5.9 x 10-12 mol. cm-2. sec-1.3. Cl-exchanged at a rate of 6-9 x 1012 mol. cm-2. sec-1 from a cellular compartment having a concentration of 39-3 mm.4. The methods used for the evaluation of Na+ movements over-estimate both influx and efflux values. If an average value of flux of 9-2 x 10-12 mol.cm-2. sec-1 is considered we obtain, PNa /PK+ ratios of 2-4 (-42 mV) or 1-3 (-57 6 mV), which are too high and do not correspond to electrophysiological evidence.5. The relative permeabilities PC1-/IPK in the case of a membrane potential of -42-0 mV could be 0-8, or 1-3 in the case of a membrane potential of -57 6 mV.6. Both conductances GK+ and GCj1 seem to play an important role in determining membrane conductance.

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“…On the other hand the Cl-makes an important contribution to the membrane conductance of rat myometrium. This confirms the findings of Csapo & Kuriyama (1963) and Abe (loc. cit.…”

Section: Discussionsupporting

confidence: 81%

Ionic fluxes in rat uterine smooth muscle.

Hamon¹,

Papadimitriou²,

Worcel³

1976

The Journal of Physiology

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“…Since membrane resistance of mouse myometrium is decreased by carbachol (and oxytocin), it is probable that permeability increase to ions underlies the depolarization, the concept being predictable from the experimental results of previous investigators (KURIYAMA, 1961;CSAPO and KURIYAMA, 1963;BULBRING and KURIYAMA, 1963;MARSHALL, 1962MARSHALL, , 1968. It is rather surprizing that in mouse myometrium the peak depolarization, which may be more hyperpolarized than in the ileal smooth muscle of guinea pigs, was identical for large concentrations of carbachol and oxytocin (Figs.…”

Section: Fig7 Spontaneous Activities Of Intact Muscle Strip (A) Andmentioning

confidence: 82%

Electrophysiological Comparison of the Action of Oxytocin and Carbachol on Pregnant Mouse Myometrium

Osa

Taga

1973

Jpn.J.Physiol

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Summary Effects of oxytocin and carbachol on the membrane potential, membrane conductance, and spike potential of pregnant mouse myometrium were investigated in isotonic and hypertonic solutions. 1) In an isotonic solution low concentrations of oxytocin (10-6-10-5 U/ml) did not cause any change in the membrane potential or in the amplitude of the action potential, but facilitated the conduction of excitation. With these concentrations, spontaneous contractions of truncated pattern became full-size. 2) Intermediate concentrations of oxytocin (10-4 U/ml) and carbachol (10-5 g/m1) caused a depolarization and an acceleration of spike activity. Higher concentration of oxytocin or carbachol caused a peak depolarization to about -25 mV. During the recovery in normal solution, membranes showed oscillatory slow waves.

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“…(a) .50 mV The spontaneously generated slow potential changes. The spontaneous generation of slow potential changes in smooth muscle has been observed by many investigators in many species and tissues (intestinal smooth muscle: Biilbring, 1957;Biilbring, Burnstock & Holman, 1958;Prosser, 1962;Bortoff, 1961;Gillespie, 1962;Biilbring & Kuriyama, 1963;uterus: Marshall, 1962;Goto, Kuriyama & Abe, 1961;Kuriyama, 1962;Csapo & Kuriyama, 1963;ureter: Kobayashi & Irisawa, 1964;Kuriyama et al 1967a). …”

Section: Resultsmentioning

confidence: 99%

Electrophysiological study of the intestinal smooth muscle of the guinea‐pig

Kuriyama¹,

Osa²,

Toida³

1967

The Journal of Physiology

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SUMMARYThe membrane properties of single cells of intestinal smooth muscle of duodenum, jejunum, ileum, caecum and rectum of guinea-pig have been studied.1. The membrane potentials of longitudinal muscles of the duodenum, jejunum, ileum, caecum and rectum varied from 54 to 56 mV and those of circular muscles of jejunum, ileum, caecum and rectum varied from 57 to 60 mV. The ablated longitudinal muscle had a slightly lower value (50 mV) than the intact one.2. The longitudinal muscle generated spontaneous discharges but these were rare in the circular muscles of the intestine except for the caecum. Overshoot potentials could be observed in all regions of the intestine. The maximum rate of rise of the spontaneously discharging longitudinal muscles varied from 11 to 18 V/sec.3. Not all of the slow potential changes (but at least some) were generated by the nervous elements distributed between the muscle layers and in them.4. The conduction velocities measured from the longitudinal muscles of jejunum and rectum in the presence of tetrodotoxin were 2*1 cm/sec and 4 0 cm/sec respectively. 5. Chronaxies of the longitudinal muscles of jejunum and rectum were 2-5 msec and 5-18 msec respectively.6. Intracellular stimulation of the single cells of the duodenum and caecum could trigger a spike, similar to that observed in the taenia coli. The spikes were mostly graded ones; a spike of full size was rarely elicited. When the spikes were triggered, the after-hyperpolarization appeared consistently presumably caused by the increased potassium conductance.7. The effective membrane resistance and the time constant were measured for the longitudinal muscles of the jejunum and rectum. When spikes were generated by intracellular stimulation the observed values were H. KURIYAMA, T. OSA AND N. TOIDA 40-50 MQ and 3-5 msec in both tissues. These values were the same as those observed in the taenia coli.8. When the time constant of the membrane was measured by the extracellular polarizing method, the longitudinal muscles of the jejunum especially and the rectum had smaller time constants than the taenia coli.9. The differences of conduction velocity and chronaxie of the different regions of the intestine are discussed in relation to the cable properties of the tissues which are directly influenced by the morphological arrangements of the tissues.

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Effects of ions and drugs on cell membrane activity and tension in the postpartum rat myometrium

Cited by 38 publications

References 25 publications

Ionic fluxes in rat uterine smooth muscle.

Ionic fluxes in rat uterine smooth muscle.

Electrophysiological Comparison of the Action of Oxytocin and Carbachol on Pregnant Mouse Myometrium

Electrophysiological study of the intestinal smooth muscle of the guinea‐pig

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