Effects of Intravenous Anesthetics on Renal Ischemia/Reperfusion Injury

Yuzer, Husamettin; Yüzbaşıoğlu, Mehmet Fatih; Çıralık, Harun; Kurutaş, Ergül Belge; Özkan, Orhan Veli; Bülbüloğlu, Ertan; Atlı, Yalçın; Erdoğan, Özgür; Kale, İlhami Taner

doi:10.1080/08860220902779962

Cited by 38 publications

(36 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thiopental is a highly lipid-soluble anesthetic, which has indicated antioxidant effect by inhibiting lipid peroxidation in ischemia/reperfusion injury [17] . Protective effects of thiopental for renal ischemia/reperfusion injury was searched in several and compared with the other intravenous agents [13,17,23] . Yüzer et al [13] reported that propofol and thiopental anesthesia protects against biochemical, and morphological damage better than control in renal I/R injury.…”

Section: Discussionmentioning

confidence: 99%

“…Protective effects of thiopental for renal ischemia/reperfusion injury was searched in several and compared with the other intravenous agents [13,17,23] . Yüzer et al [13] reported that propofol and thiopental anesthesia protects against biochemical, and morphological damage better than control in renal I/R injury. They also reported in the same study; thiopental decreases the kidney I/R damage via free oxygen radicals in rats, and this effect is significant compared to etomidate [13] .…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Tubular epithelial cell damage and ischemic acute renal failure can occur due to renal I/R damage, and this is accompanied by high rates of mortality [13] . The protective effects of various intravenous anesthetics, such as ketamine, propofol, etomidate, and thiopental [14][15][16] , against renal I/R damage have been studied [12,13] . Thiopental is known to reduce the release of free oxygen radicals from neutrophils in renal I/R damage, inhibit lipid peroxidation, and demonstrate antioxidant effects by reducing hemolysis of red blood cells caused by free oxygen radicals [17] .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Çoşkuner¹,

Türkay²,

Yıldız³

et al. 2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

The aim of this study was to determine optimal protective thiopental dose in renal ischemia/reperfusion (I/R) damage. This study was carried out on 42 rats. After abdominal midline incision, left renal artery was occluded. After 60 min of ischemia and reperfusion, a left nephrectomy was performed. Surgical intervention was made for the six rat groups (excluding sham group). In control group, 60 minutes of ischemia and reperfusion were applied to left kidneys. Thiopental (3, 10, 30, 60, 80 mg/ kg) was applied intra-peritoneally to the rats except sham and control groups 15 minutes before reperfusion. After reperfusion, IL-1α, IL-6, TNF-α levels; SOD activity; MDA and NO levels were measured. There was no decrease in IL-6 or TNF-α levels in groups at all doses. IL-1α levels only decreased in the group receiving 3 mg/kg thiopental. Only the group receiving 80 mg/kg observed decrease in MDA levels. The rats receiving 30 mg/kg showed significant increase in SOD levels. The rats receiving 3 mg/ kg detected significant increase in glutathione peroxidase (GPx) levels. The groups receiving 10, 30, and 60 mg/kg thiopental showed decrease in nitric oxide (NO) levels. According to study results, different doses of thiopental affected each biochemical indicator in renal I/R damage. Keywords: Renal Ischemia/Reperfusion, Thiopental, Rat Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi ÖzetÇalışmada ratlarda farklı dozlarda uygulanan tiyopentalin renal I/R hasarına karşı koruyucu optimal dozunun saptanması amaçlandı. Bu çalışma 42 ratta yapıldı. Abdominal orta hat insizyonu sonrası sol renal arter okluzyonu yapıldı. Altmış dakika işemi ve reperfüzyondan sonra sol nefrektomi yapıldı. Altışar rattan oluşan gruplarda (sham grup dışında) cerrahi prosedür uygulandı. Kontrol grubunda sol böbreklere 60 dk işemi ve reperfüzyon uygulandı. Sham ve kontrol grubu dışındaki ratlara reperfüzyondan 15 dk önce tiyopental (3, 10, 30, 60, 80 mg/kg) intra-peritoneal olarak uygulandı. Reperfüzyon sonunda IL-1α, IL-6, TNF-α seviyeleri; SOD aktivitesi; MDA ve NO düzeyleri ölçüldü. Gruplarda IL-6 ve TNF-α seviyelerinde azalma olmadı. Yalnızca 3 mg/kg tiyopental uygulanan grupta IL-1α seviyesi azaldı. MDA düzeylerinde düşme yalnızca 80 mg/kg tiyopental uygulanan grupta görüldü. 30 mg/ kg tiyopental uygulanan ratlarda SOD düzeylerinde anlamlı yükseklik saptandı. 3 mg/kg tiyopental uygulanan ratlarda glutatyon peroksidaz (GPx) düzeylerinde anlamlı yükseklik saptandı. Nitrik oksit seviyesindeki düşme 10, 30 ve 60 mg/kg tiyopental uygulanan gruplarda görüldü. Çalışma sonuçlarına göre, renal işemi reperfüzyon hasarında her bir biyokimyasal belirteci tiyopentalin farklı dozları etkiledi.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Çoşkuner¹,

Türkay²,

Yıldız³

et al. 2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

show abstract

“…[8] Simultaneously, the release of oxygen free radicals and proteolytic enzymes may cause large numbers of oxygen free radicals again and more serious organ damage. [9] This may be an important mechanism that causes pancreatic injury and induces MODS in the process of ARF.…”

Section: Tablementioning

confidence: 99%

Pancreatic Injury in Rabbits with Acute Renal Failure

et al. 2009

View full text Add to dashboard Cite

Background. Acute renal failure (ARF) is a common critical disorder. To decrease the mortality, it is important to prevent ARF from invading other organs in clinical setting. It is not known whether there is a dysfunction in pancreas during the pathogenesis of ARF. This study aimed to investigate the changes of morphology and function on pancreas in ARF rabbits. Methods. Sixty rabbits were randomly divided into four groups. The ARF model of groups 1 and 2 rabbits was established by hypodermic injection of 1% HgCl 2 (1.3 mL/kg) and intramuscular injection of 50% glyceritum (10 mL/kg), respectively. The control groups 1 and 2 were injected with same volume of normal saline. After 24 hours, urea and creatinine contents and pancreatic amylase (AMY) activities in serum were measured using an automatic biochemical analyzer; the insulin levels were measured with radioimmunoassay method. Moreover, morphological alterations were examined by light microscopy; free radicals, nitric oxide (NO), and nitric oxide synthase (NOS) in pancreas homogenate were determined. Results. Morphological study showed that there were vacuolar degeneration and necrosis in pancreas of ARF for both groups 1 and 2. Compared with corresponding control group, the AMY activity was significantly elevated, whereas the INS values were decreased significantly in ARF groups 1 and 2. Malonaldehyde, NO, and NOS in pancreas homogenate were significantly increased, and superoxide dismutase activity was decreased. Conclusion. These data suggested that there were morphological damage of pancreas and disturbance of pancreatic secretion function in rabbits with ARF. Free radicals-injury and NO excessive release may explain the observed dysfunction.

show abstract

Preparation and Monitoring of Small Animals in Renal MRI

Kaucsár

Hosszú

Seeliger

et al. 2021

Methods in Molecular Biology

View full text Add to dashboard Cite

Renal diseases remain devastating illnesses with unacceptably high rates of mortality and morbidity worldwide. Animal models are essential tools to better understand the pathomechanism of kidney-related illnesses and to develop new, successful therapeutic strategies. Magnetic resonance imaging (MRI) has been actively explored in the last decades for assessing renal function, perfusion, tissue oxygenation as well as the degree of fibrosis and inflammation. This chapter aims to provide an overview of the preparation and monitoring of small animals before, during, and after surgical interventions or MR imaging. Standardization of experimental settings such as body temperature or hydration of animals and minimizing pain and distress are essential for diminishing nonexperimental variables as well as for conducting ethical research.This publication is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers.

show abstract

Effects of Intravenous Anesthetics on Renal Ischemia/Reperfusion Injury

Cited by 38 publications

References 17 publications

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Pancreatic Injury in Rabbits with Acute Renal Failure

Preparation and Monitoring of Small Animals in Renal MRI

Contact Info

Product

Resources

About