Effects of intermittent chlorination on plasma proteins of rainbow trout (Salmo gairdneri)

Assessment of serum protein fractions in rainbow trout using automated electrophoresis and densitometry

“…Fibrinogen was identified in the 12th band (of 13) by Perrier et al 3 by comparison of plasma and serum on a disk electrophoretic tracing. Booth et al 11 found that the same band 12 disappeared in chlorine‐exposed rainbow trout and correlated this finding with severe hemorrhage in the exposed fish. Fibrinogen is an important acute‐phase protein slightly trailing the β 2 fraction in mammals.…”

“…Unfortunately, neither the electrophoretic techniques nor the classification or the nomenclature of serum protein fractions have been standardized in rainbow trout, leading to conflicting data and difficulty in obtaining clinically useful values 10 . In addition, little work has been performed to evaluate protein patterns of fish with pathologic conditions 2,4,9,11 . The aim of the present study was to evaluate preliminarily the serum protein fractions of rainbow trout using automated cellulose acetate electrophoresis and densitometry, with particular attention to interindividual differences.…”

“…Deutsch and McShan 1 obtained 8 protein fractions as a result of “free electrophoresis” in serum from rainbow trout ( O. mykiss ). Disk gel electrophoresis resulted in 13 main bands in rainbow trout plasma, 3,6,11 although additional bands were also reported to occur occasionally 3 . Agarose gel electrophoresis of rainbow trout plasma resulted in 11 protein fractions (3 in the albumin zone, 4 in the α‐globulin zone, 3 in the β‐globulin zone, and 1 in the γ‐globulin zone).…”

“…10 In addition, little work has been performed to evaluate protein patterns of fish with pathologic conditions. 2,4,9,11 The aim of the present study was to evaluate preliminarily the serum protein fractions of rainbow trout using automated cellulose acetate electrophoresis and densitometry, with particular attention to interindividual differences.…”