Effects of Intermittent Administration of Parathyroid Hormone (1‐34) on Bone Differentiation in Stromal Precursor Antigen‐1 Positive Human Periodontal Ligament Stem Cells

Abstract

Periodontitis is the most common cause of tooth loss and bone destruction in adults worldwide. Human periodontal ligament stem cells (hPDLSCs) may represent promising new therapeutic biomaterials for tissue engineering applications. Stromal precursor antigen-1 (STRO-1) has been shown to have roles in adherence, proliferation, and multipotency. Parathyroid hormone (PTH) has been shown to enhance proliferation in osteoblasts. Therefore, in this study, we aimed to compare the functions of STRO-1(+) and STRO-1(−) hPDLSCs and to investigate the effects of PTH on the osteogenic capacity of STRO-1(+) hPDLSCs in order to evaluate their potential applications in the treatment of periodontitis. Our data showed that STRO-1(+) hPDLSCs expressed higher levels of the PTH-1 receptor (PTH1R) than STRO-1(−) hPDLSCs. In addition, intermittent PTH treatment enhanced the expression of PTH1R and osteogenesis-related genes in STRO-1(+) hPDLSCs. PTH-treated cells also exhibited increased alkaline phosphatase activity and mineralization ability. Therefore, STRO-1(+) hPDLSCs represented a more promising cell resource for biomaterials and tissue engineering applications. Intermittent PTH treatment improved the capacity for STRO-1(+) hPDLSCs to repair damaged tissue and ameliorate the symptoms of periodontitis.

MicroRNA-214 Suppresses Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting ATF4

“…hPDLCs were harvested and cultured as previously reported [ 29 ]. Tissues attached to the middle third of tooth root was collected and cut into small pieces (1 mm 3 ) followed by digestion with 3 mg/mL type 1 collagenase (Life Technologies, Carlsbad, CA, USA) and 4 mg/mL dispase (Life Technologies) at 37°C for 1 h. The tissues were cultured in complete α -minimum essential medium (Gibco, Grand Island, NY, USA) containing 10% ( v / v ) fetal bovine serum (Gibco), 100 U/mL penicillin, 100 μ g/mL streptomycin (HyClone, Logan, UT, USA), and 5 mM L-glutamine (Gibco).…”

Oestrogen retains human periodontal ligament stem cells stemness in long‐term culture

“…Approval was granted by the Sun Yat‐sen University Research Ethics Committee. Human periodontal ligament stem cells were isolated and collected as previously described . Briefly, hPDLSCs were isolated from periodontal tissue in the middle one third of the root, digested with 3 mg/mL collagenase type I and 4 mg/mL dispase (Gibco‐BRL, Grand Island, NY, USA) for 1 hour at 37°C.…”

“…Human periodontal ligament stem cells were isolated and collected as previously described. 4,16 Briefly, hPDLSCs were isolated from periodontal tissue in the middle one third of the root, digested with 3 mg/mL collagenase type I and 4 mg/mL dispase (Gibco-BRL, Grand Island, NY, USA) for 1 hour at 37°C. Colony-forming cells were collected and cultured in phenol redfree alpha modified Eagle medium (α-MEM; Gibco-BRL) supplemented with 10% charcoal-treated (Gibco-BRL), 100 μg/mL streptomycin, 100U/mL penicillin (HyClone, Logan, UT, USA), and 5 mmol/Lglutamine (Gibco-BRL) at 37°C in 5% CO 2 .…”

Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering

“…It was thought that a homogeneous source of stem cells might provide a better source for therapeutic purposes compared to unsorted heterogeneous cells. MSCs share a common surface marker expression profile, whereas STRO-1, a widely used MSC surface marker, distinguishes only a subpopulation of mesenchymal stem cells that are unique in terms of adherence, proliferation and multilineage differentiation potential [13]. pTGSCs exhibited the common surface marker profile of MSCs and kept their surface marker expression profile up to passage 5 without any significant differences in percentages of markers' expressions except CD105 whose expression decreased with passaging as previously reported for human MSCs elsewhere [14].…”