Effects of inhibition of P2Y1 and P2Y12 on whole blood clotting, coagulum elasticity and fibrinolysis resistance studied with free oscillation rheometry

Abstract

Introduction: In vivo, initial platelet activation is likely caused by platelet contacts with

Platelet quality after washing: the effect of storage time before washing

“…Rheologic changes occurring during clot formation and clot retraction were studied by FOR with a rheometer (ReoRox 4, Medirox AB, Nyköping, Sweden). When analyzing clotting time and clot retraction (clot elasticity, clot strength) the sample is added to a reaction chamber which consists of a gold‐coated sample cup with a gold‐coated cylinder (bob) attached to a shaft in the center of the cup 11 . When the reaction chamber is placed in the rheometer, the sample cup starts to oscillate.…”

The quality of platelet concentrates produced by COBE Spectra and Trima Accel cell separators during storage for 7 days as assessed by in vitro methods

“…In FOR, a sample cup is set into free oscillation and the change in frequency and damping of the oscillation caused by the coagulating sample is registered over time. Measurement of change in G′ over time in PLT‐rich plasma samples requires the use of a reaction chamber that consists of a gold‐coated sample cup with a gold‐coated cylinder (bob) attached to a shaft in the center of the cup 6,9 . When using the reaction chamber, the changes occurring in frequency and damping during the coagulating phase and the clot retraction phase are registered, and the change in G′ is calculated from the frequency and damping data as previously described 7 .…”

The effect of gamma irradiation on the quality of apheresis platelets during storage for 7 days

“…In the rheometer, a sample cup oscillates and the change in the oscillation frequency and damping caused by the coagulating sample is registered optically over time. G′ measurement of PLT‐rich plasma samples requires the use of a reaction chamber consisting of a gold‐coated sample cup with a gold‐coated cylinder (bob) 9 . The changes in G′ are calculated in the SI unit Pascal (Pa) from the frequency and damping data as previously described 6 .…”