Effects of in vivo hyperoxia on erythrocytes. 3. In vivo peroxidation of erythrocyte lipid.

Tocopherol transport in the rat erythrocyte

“…These data may be relevant to clinical situation involving tocopherol, since several recent reports (22)(23)(24) indicate that lipid peroxides accumulate in the tocopherol deficient red cell in animals and man. Increased lipid peroxide formation has also been described in cells of patients with paroxysmal nocturnal hemoglobinuria, despite normal tocopherol levels (25).…”

Aspirin-induced Hemolysis: The Role of Concomitant Oxidant (H2O2) Challenge

“…The chromagens associated with lipid peroxide formation, primarily malonaldehyde (17,24,29). were determined as follows: a 5% RBC suspension was exposed to an equal volume of ASA (50 mg/100 ml) and H202 (2.4%) as described previously, after which I ml of this mixture was added to 1 ml 10% trichloroacetic acid.…”

Studies of Paroxysmal Nocturnal Hemoglobinuria Erythrocytes: Increased Lysis and Lipid Peroxide Formation by Hydrogen Peroxide*

“…Iron serves as a catalyst in the peroxidation of lipid (13,14), and increased levels of lipid peroxides have been found in tissues of animals injected with iron complexes (15). Recent studies in our laboratory have shown that peroxidation of red cell lipid can occur in vivo causing damage and lysis of red cells (16). These observations led us to speculate that PNH red cells might be unusually sensitive to lipid prooxidants, and that lipid peroxidation might be involved in PNH hemolysis.…”

“…Details of the method used for determining in vitro lytic sensitivity of erythrocytes to hydrogen peroxide have been described previously (16,20,21). All glassware used was washed with concentrated nitric acid and hydrogen peroxide (1000 ml of acid plus 5 ml of 30% H202 and thoroughly rinsed with thrice deionized water.…”

“…Red cell glutathione peroxidase activity was determined by a method described previously (16) and blood reduced glutathione was measured as described by Beutler et al (28).…”