Effects of in vivo Hyperoxia on Erythrocytes IV. Studies in Dogs Exposed to Hyperbaric Oxygenation.

Inhibition of Erythrocyte Acetylcholinesterase by Peroxides*

“…We subsequently observed both hemolysis and a fall of erythrocyte AChE activity after in vivo exposure to OHP in one patient (Mengel et al, 1965) and a consistent decrease of erythrocyte AChE activity in dogs exposed to OHP (Zirkle et al, 1965).…”

“…He developed hemolytic anemia and a fall of red cell acetylcholinesterase (AChE) after a brief exposure to OHP (Mengel et al, 1965). The recent observation that red cell AChE was consistently decreased in dogs after exposure to OHP (Zirkle et al, 1965) prompted us to carry out in vitro Director, Division of Hematology, Ohio State University, Columbus, Ohio. § Medical Student, Duke University Medical Center. 1 Abbreviations used in this work: AChE, acetylcholinesterase;…”

Effects of in vivo hyperoxia on erythrocytes. 3. In vivo peroxidation of erythrocyte lipid.

“…With regard to this possibility, we have demonstrated changes in human erythrocyte glycolytic intermediates after prolonged in vivo exposure to OHP that suggested inhibition of the sulfhydryl-bearing enzyme glyceraldehyde 3-phosphate dehydrogenase (65). We have also observed decreased activity of erythrccyte acetylcholinesterase (presumed to be a sulfhydryl-bearing enzyme) in dogs exposed to OHP (66).…”

Studies of Paroxysmal Nocturnal Hemoglobinuria Erythrocytes: Increased Lysis and Lipid Peroxide Formation by Hydrogen Peroxide*

“…Alternatively, lysis could reflect a secondary effect of lipid peroxide formation, since the damaging effect of lipid peroxides on proteins, enzymes, and metabolic pathways has been well documented (50)(51)(52). With regard to this possibility, we have observed decreased activity of erythrocyte acetylcholinesterase (presumed to be a sulfhydryl-bearing enzyme) in dogs exposed to oxygen under high pressure (53), and have established the fact that this change was an effect of lipid peroxides (54).…”