Effects of in vitro tissue culture conditions and acclimatization on the contents of Rubisco, leaf soluble proteins, photosynthetic pigments, and C/N ratio

Premkumar, A.; Mercado, José A.; Quesada, Miguel A.

doi:10.1078/0176-1617-00214

Cited by 40 publications

(27 citation statements)

References 37 publications

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“…In the present experiment F v /F m values of control leaves were comparatively low in the range of c. 0.71 (Table 2), which is distinctly lower than the value of 0.82 commonly reported for non-stressed control leaves. However, the here recorded value may be considered typical for in vitro plants under the present environmental conditions, because in vitro plants are generally characterized by smaller values (Premkumar et al, 2001).…”

Section: Discussionmentioning

confidence: 98%

Chlorophyll Fluorescence as a Tool to Assess the Regeneration Potential of African Violet Leaf Explants

Keutgen

Figas

Tomaszewska-Sowa

et al. 2016

Not Bot Hort Agrobot Cluj

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Micropropagation of many ornamentals has enabled their efficient commercialisation and many problems have been solved by the elaboration of adequate culture protocols. Nevertheless, a non-destructive technique for monitoring the developmental progress of explants would be desirable. The present study focussed on the applicability of chlorophyll fluorescence in leaf explants of African violet (a Saintpaulia ionantha × confusa -hybrid) explanted onto Murashige and Skoog basic medium. The explants that survived on the medium without additional phytohormones had the capacity to develop further into two different kinds of explants: light green explants, characterized by a non-regular size growth and stiffer appearance, and dark green explants capable of organogenesis. Compared to the source leaves of African violet plants, explants were characterized by reduced chlorophyll (Chl) and carotenoid (Car) contents as well as a tendency towards a higher Car/Chl ratio. The Chl a/b ratio decreased significantly in the light green explants. A reduction of maximum quantum efficiency of photosystem II (F v /F m ) accompanied by a high percentage (> 50%) of thermal energy dissipation as a consequence of an elevated light intensity (800 µmol m -2 s -1 quanta) indicated photoinhibition in the light green explants, whereas in the dark green explants the largest percentage (> 50%) of the light energy was dissipated into the fraction of photon energy trapped by 'closed' photosystem II reaction centres. These results suggest that the capacity of organogenesis of leaf explants of African violet can be monitored using chlorophyll fluorescence, because it is related to modifications of the photosynthetic system.

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Section: Discussionmentioning

confidence: 98%

Chlorophyll Fluorescence as a Tool to Assess the Regeneration Potential of African Violet Leaf Explants

Keutgen

Figas

Tomaszewska-Sowa

et al. 2016

Not Bot Hort Agrobot Cluj

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“…By the way, the leaf area does not seem to be a suitable variable for to be used to evaluate the plantlets acclimatization, although Premkumar et al (2001) have pointed out that the success of acclimatization depends on the sources inside the developing plantlets. According to Ticha et al (1998), the addition of sucrose to the culture medium influenced positively the increase of biomass and leaf area as well as accumulation of chlorophyll and photosynthetic capacity in tobacco plantlets.…”

Section: Discussionmentioning

confidence: 99%

“…According to Premkumar (2001), while low light inhibits chloroplasts development, sugars content in the culture medium may restrain the photosynthetic enzymes activity, as for instance the Rubisco (EC 4.1.1.39). Watanabe et al (1990); Tanaka et al (1991); Rival et al (1999), observed that Chrysantemum morifolium L., Spathiphyllum wallisi Regel and coconut plantlets grown in vitro with 3% sucrose, presented low Rubisco levels, what is characteristic for in vitro plantlets.…”

Section: Discussionmentioning

confidence: 99%

Monitoring the end of the in vitro phase of Anthurium andreanum Lindl. plantlets

Stancato¹,

Tucci²

2010

Braz. J. Plant Physiol.

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Estimulation of autotrophy in in vitro plantlets could be achieved through changes in the culture medium, or by changing the traditional hermetic caps by one that could allow gas exchanges between the culture and the environment. Besides that, the use of lamps with distinct emission spectrum irradiaction has propitiated successful results. This work was carried out aiming to evaluate the either the combined or the single action of some factors that can induce autotrophy on in vitro A. andraeanum cv. Eidibel plantlets. 3 sucrose concentrations were used: 0, 15 and 60 mM and for each one, to kinds of flasks according to the cap ventilation: under (0.038 L.h ) and without ventilation. Flasks were kept under cold light fluorescent lamps or under gro-lux lamps. At the end of the experiment showing the highest shoot dry mass treatment was 60 mM, under ventilation and gro-lux, and the treatment which accumulate root dry mass to a lesser extent were 0 mM with ventilation and cold light and 15 mM without ventilation and cold light. In average, treatments with higher sucrose content in the culture medium, that is, 60 mM, under gro-lux lamps, presented the highest chlorophyll a, b and total contents, than those under cold lamp. Steps of carbohydrates metabolism could be associated with the total soluble sugars (sucrose and reducing sugars) levels, highlighting the steps where nutrient requirements were higher, showing the role of the plantlets sink.Key words: acclimatization, Araceae, plantlets metabolism resuMo Monitoramento do final da fase in vitro de plântulas de Anthurium andraeanum. O estímulo à autotrofia em plântulas in vitro pode ser alcançado através de mudanças no meio de cultura, ou pela troca de tampas herméticas por tampas que permitam a troca de gases entre a cultura e o ambiente. Além disso, o uso de lâmpadas com distintos espectros de irradição tem propiciado bons resultados. Este trabalho foi conduzido com o objetivo de avaliar a ação isolada ou em conjunto de fatores que podem induzir a autotrofia em plântulas de A. andraeanum vc. Eidibel in vitro. 3 concentrações de sacarose foram usadas: 0, 15 e 60 mM e para cada concentração foram empregados frascos com ventilação (0,038 L.h -1 ) ou sem ventilação. Os frascos foram mantidos sob lâmpada fluorescente fria ou gro-lux. O final dos experimentos mostrou que o maior acumulo de massa seca da parte aérea ocorreu a 60 mM, sob ventilação e gro-lux, e os tratamento que acumularam menor massa seca de raízes foram 0 mM com ventilação e sob luz fria e 15 mM sem ventilação e luz fria. Em média, os tratamentos com o maior teor de sacarose no meio, 60 mM, sob lâmpada gro-lux, apresentaram as maiores concentrações de clorofila a, b e total, do que sob luz fria. Os níveis de açúcares solúveis totais (sacarose e açúcares redutores) mostraram passos do metabolismo de carboidratos nessas plantas, realçando os momentos em que a exigência por nutrientes foi maior, destacando-se o papel de dreno das plântulas. palavras-chave: aclimatização, Araceae, metabolismo de plân...

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“…Las plántulas de E. platyacanthus germinadas en Gelrite registraron los valores más bajos en el contenido de pigmentos fotosintéticos, muy probablemente ocasionado por la hiperhidratación de los tejidos, fenómeno que influye en la eficiencia fotosintética, ya que la estructura de los cloroplastos es defectuosa y el contenido de clorofila bajo, lo que se reflejará en una menor tasa fotosintética (Reil y Berger, 1997, Kevers et al, 2004. El contenido de clorofila en las plantas in vitro puede ser explicado por la presencia de citocininas y carbohidratos exógenos en el medio que las hacen dependientes de una fuente de carbono, así mismo se ha reportado la escasa actividad de la Rubisco, debido a alteraciones en el nivel de nitrógeno o del nivel de compuestos fotosintéticos (Ladyman y Girard, 1992;Premkumar et al, 2001;Ivanova y Van Staden, 2011).…”

Section: Pigmentos Fotosintéticosunclassified

Efecto De Diferentes Agentes Gelificantes en La Germinación Y Desarrollo in Vitro De Plántulas De Echinocactus Platyacanthus Link Et Otto (Cactaceae)

2016

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Effects of in vitro tissue culture conditions and acclimatization on the contents of Rubisco, leaf soluble proteins, photosynthetic pigments, and C/N ratio

Cited by 40 publications

References 37 publications

Chlorophyll Fluorescence as a Tool to Assess the Regeneration Potential of African Violet Leaf Explants

Chlorophyll Fluorescence as a Tool to Assess the Regeneration Potential of African Violet Leaf Explants

Monitoring the end of the in vitro phase of Anthurium andreanum Lindl. plantlets

Efecto De Diferentes Agentes Gelificantes en La Germinación Y Desarrollo in Vitro De Plántulas De Echinocactus Platyacanthus Link Et Otto (Cactaceae)

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