Effects of in vitro growth culture duration and prematuration culture on maturational and developmental competences of bovine oocytes derived from early antral follicles

Huang, Weiping; Nagano, Masashi; Kang, Seung-Ji; Yanagawa, Yuchio; Takahashi, Yoshiyuki

doi:10.1016/j.theriogenology.2013.07.004

Cited by 42 publications

(38 citation statements)

References 31 publications

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“…Mitochondrial activity is one of indicators of cytoplasmic maturation of oocytes (4). Huang et al (19,20) reported that in vitro-grown oocytes acquired high mitochondrial activity after 10-h prematurational culture, and showed higher maturation and development competences compared to oocytes without prematurational culture. Jeseta et al (24) reported that the kinetics of mitochondrial activity and adenosine triphosphate (ATP) production during maturational culture were influenced by the atretic grade of granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

“…After 3 washes in physiological saline, sliced ovarian cortex tissues (<1 mm in thickness) were prepared using a surgical blade (No. 11) and stored in TCM-199 (Invitrogen, Grand Island, NY, USA) supplemented with 0.1% polyvinyl alcohol, 25 mM HEPES, 10 mM sodium bicarbonate, and 50 mg/mL gentamicin sulfate (isolation medium, pH 7.4, at 37°C), as described by Huang et al (19). Under a stereomicroscope, early antral follicles (0.5-1.0 mm in diameter) were dissected from the sliced ovarian tissues using a surgical blade (No.…”

Section: Collection Of Early Antral Follicles and Growth Culture Of Omentioning

confidence: 99%

“…The growth medium consisted of HEPES-buffered TCM-199 (Invitrogen) supplemented with 0.91 mM sodium pyruvate, 1 μg/ mL estradiol-17β (E 2 ), 5% fetal calf serum (FCS; Invitrogen), 4 mM hypoxanthine, 4% polyvinylpyrrolidone (MW 360,000), and 50 μg/mL gentamicin sulfate. At the onset of growth culture, OGCs were photographed under an inverted microscope (CK 40; Olympus, Tokyo, Japan) attached with a CCD camera (Moticam 2000; Shimadzu Rika Corporation, Tokyo, Japan), and viability of OGCs was determined by their morphologies as previously reported (19). During growth culture, half (100 μL) of the growth medium was replaced with the same amount of fresh medium every 4 days.…”

Section: Collection Of Early Antral Follicles and Growth Culture Of Omentioning

confidence: 99%

“…For investigating oocyte quality correctly, we should use oocytes derived from follicles before degradation. Recently, we reported that in vitro-grown bovine oocytes derived from early antral follicles, just before recruitment to follicular waves (0.5-1 mm in diameter), achieved good development to blastocysts corresponding to the development of in vivo-grown oocytes (19,20). In the present study, to investigate the relationship between ovarian reserve estimated with AFC and oocyte quality, we cultured bovine oocyte-granulosa complexes (OGCs) collected from early antral follicles in ovaries with high and low AFCs.…”

Section: Collection Of Early Antral Follicles and Growth Culture Of Omentioning

confidence: 99%

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The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles

et al. 2016

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To clarify the relationship between ovarian reserve and the developmental competence of bovine oocytes, oocyte-granulosa complexes (OGCs) collected from early antral follicles (≤1 mm) in ovaries with high (≥25) and low (<25) antral follicle counts (AFCs) were used. OGCs derived from different AFC groups were cultured for growth followed by maturation, fertilization and blastocyst formation. Viability of OGCs during growth culture was similar between groups; however, OGCs in the high-AFC group had a larger number of granulosa cells than the low-AFC group at 12 days of growth. The proportion of matured oocytes in the high-AFC group was higher than that in the low-AFC group. Mitochondrial activity of oocytes before maturation in the high-AFC group was higher than that in the low-AFC group; however, accumulation of reactive oxygen species was similar between groups. Cleavage rate in the high-AFC group tended to be higher than that in the low-AFC group, although blastocyst development was similar between groups. In conclusion, oocytes derived from ovaries with high AFC have higher maturational ability and fertilizability than those from low AFC. The difference may be caused by high proliferation of granulosa cells from ovaries with high AFC.Major functions of ovaries are producing fertilizable oocytes having developmental competence that result in successive conception and secreting sex steroid hormones that induce the estrous cycle and sustain pregnancy. Ovarian reserve is defined as potential ability of these functions (38), and is proposed as a factor of the fertility for mammalian female animals including humans (6) and cattle (22). The number of small antral follicles (antral follicle count; AFC) in a pair of ovaries detected by ultrasonography is considered as an indicator of ovarian reserve (7), because it correlates with the number of primordial follicles (23) and is stable in individual animals (1, 10). During the long lifetime of women, ovarian reserve decreases as aging. Therefore, the relationship between oocyte quality and ovarian reserve in aged women is well investigated, and it is reported that aged women with small ovarian reserve had low quality oocytes (12,32). In addition, the time of onset of menopause is closely correlated with ovarian reserve; small reserve resulted in earlier menopause (11). The size of the ovarian reserve varies between individuals of the same species or strain, but the relationship between oocyte quality and small ovarian reserve of young women is not known. Therefore, we need to investigate the relationship between oocyte quality and ovarian reserve of young animals. It is reported that dairy cattle can be used as a novel experimental model for women reproduction; because cattle are single-ovulating species that have

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Section: Discussionmentioning

confidence: 99%

Section: Collection Of Early Antral Follicles and Growth Culture Of Omentioning

confidence: 99%

Section: Collection Of Early Antral Follicles and Growth Culture Of Omentioning

confidence: 99%

Section: Collection Of Early Antral Follicles and Growth Culture Of Omentioning

confidence: 99%

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The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles

et al. 2016

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“…In our previous study, we clarified that the optimal duration of IVG is 12 days, since a longer duration (14 days) of IVG culture resulted in reduced cumulus cell viability, which was related to low developmental competence of oocytes, and shorter duration (10 days) resulted in lower nuclear maturation. Furthermore, prematurational culture (pre-IVM) for 20 h in medium supplemented with phosphodiesterase (PDE) inhibitor (3-isobutyl-1-methylxanthine, IBMX) improved nuclear maturation, resulting in a high blastocyst rate (43% based on cleaved oocytes) in bovine IVG oocytes derived from early antral follicles (0.5–1 mm in diameter) [5]. However, why and how pre-IVM contributes to the improvement of developmental competence are still unclear.…”

mentioning

confidence: 99%

Prematurational Culture with 3-Isobutyl-1-methylxanthine Synchronizes Meiotic Progression of the Germinal Vesicle Stage and Improves Nuclear Maturation and Embryonic Development in In Vitro-grown Bovine Oocytes

Huang

Nagano

Kang

et al. 2014

Journal of Reproduction and Development

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The objective of this study was to clarify the effects of prematurational culture (pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95 μm in diameter) derived from early antral follicles (0.5–1 mm in diameter) were cultured for 12 days for in vitro growth (IVG). IVG oocytes with a normal appearance were subjected to examinations of diameter and chromatin structure in the germinal vesicle (GV) before IVM. In addition, percentages of metaphase II (M II) were examined after IVM. Regardless of pre-IVM, the mean diameters of IVG oocytes were about 115 μm. The proportions of GV3 (50.0%) and M II stages (80.1%) of IVG oocytes with pre-IVM were higher than those without pre-IVM (28.0 and 49.4%, respectively). In experiment 2, the fertilizability and developmental competence of IVG oocytes were examined. Regardless of pre-IVM, the normal fertilization rates of IVG oocytes were similar (around 70%) but were lower than that of in vivo-grown oocytes (88.0%). Cleavage and blastocyst rates of IVG oocytes with pre-IVM (63.0 and 26.1%, respectively) were higher than those without pre-IVM (45.8 and 12.7%, respectively). The blastocyst rate based on cleaved IVG oocytes with pre-IVM (41.7%) was similar to that of in vivo-grown oocytes (48.7%), although the cleavage rate of IVG oocytes with pre-IVM was lower than that of in vivo-grown oocytes. In conclusion, pre-IVM with IBMX improved the maturational and developmental competences of IVG oocytes, probably due to promotion of their chromatin transition and synchronization of meiotic progression.

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Role of cAMP modulator supplementations during oocyte in vitro maturation in domestic animals

Leal

Monteiro

Souza-Fabjan

et al. 2018

Animal Reproduction Science

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Effects of in vitro growth culture duration and prematuration culture on maturational and developmental competences of bovine oocytes derived from early antral follicles

Cited by 42 publications

References 31 publications

<b>The relationship between antral follicle count in a bovine ovary and developmental competence of </b><i><b>in vitro</b></i><b>-grown oocytes derived from early antral follicle</b><b>s </b>

<b>The relationship between antral follicle count in a bovine ovary and developmental competence of </b><i><b>in vitro</b></i><b>-grown oocytes derived from early antral follicle</b><b>s </b>

Prematurational Culture with 3-Isobutyl-1-methylxanthine Synchronizes Meiotic Progression of the Germinal Vesicle Stage and Improves Nuclear Maturation and Embryonic Development in <i>In Vitro</i>-grown Bovine Oocytes

Role of cAMP modulator supplementations during oocyte in vitro maturation in domestic animals

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