Role of cAMP modulator supplementations during oocyte in vitro maturation in domestic animals

Leal, Gabriela Ramos; Monteiro, C. A. S.; Souza-Fabjan, J. M. G.; Vasconcelos, Carlos Otávio de Paula; Nogueira, Luiz Altamiro Garcia; Ferreira, Ana Maria Reis; Serapião, R. V.

doi:10.1016/j.anireprosci.2018.11.002

Cited by 32 publications

(26 citation statements)

References 148 publications

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“…On the other hand, GO terms significantly enriched by host genes generating DECs in oocytes are mainly related to oocyte meiosis, such as female meiotic division, cAMP signaling, and VEGF signaling. For instance, both WEE2 enriched in the female meiotic division and cAMP molecules have been shown to inhibit the resumption of oocyte meiosis in several species [47, 48]. In contrast, VEGF supplement significantly improved maturation rate of oocytes in cattle [49], sheep [50] and pigs [51].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte maturation

Cao

Gao

et al. 2019

Aging

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Thousands of circular RNAs (circRNAs) have been recently discovered in cumulus cells and oocytes from several species. However, the expression and function of circRNA during porcine oocyte meiotic maturation have been never examined. Here, we separately identified 7,067 and 637 circRNAs in both cumulus cells and oocytes via deep sequencing and bioinformatic analysis. Further analysis revealed that a faction of circRNAs is differentially expressed (DE) in a developmental stage-specific manner. The host genes of DE circRNAs are markedly enriched to multiple signaling pathways associated with cumulus cell function and oocyte maturation. Additionally, most DE circRNAs harbor several miRNA targets, suggesting that these DE circRNAs potentially act as miRNA sponge. Importantly, we found that maternal circARMC4 knockdown by siRNA microinjection caused a severely impaired chromosome alignment, and significantly inhibited first polar body extrusion and early embryo development. Taken together, these results demonstrate for the first time that circRNAs are abundantly and dynamically expressed in a developmental stage-specific manner in cumulus cells and oocytes, and maternally expressed circARMC4 is essential for porcine oocyte meiotic maturation and early embryo development.

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Section: Discussionmentioning

confidence: 99%

Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte maturation

Cao

Gao

et al. 2019

Aging

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“…The optimal concentrations of MAM reagents in different studies are not consistent (Ramos Leal et al, 2018). In our study, GVBD was inhibited by relatively higher doses of single reagents and lower doses of combined reagents.…”

Section: Discussionmentioning

confidence: 98%

“…Previous findings showed that lower concentrations of forskolin caused less DNA damage in oocytes (Paschoal et al, 2016) meiotic arrest. Levels of cAMP inside oocytes can be increased after treatment with MAM reagents (Ramos Leal et al, 2018). A high level of cAMP in the cytoplasm may lead to a corresponding increase in the mitochondrial matrix, which, in turn, affects mitochondrial function (Di Benedetto, Gerbino, & Lefkimmiatis, 2018;Ould Amer & Hebert-Chatelain, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The level of cAMP is controlled through modulation of its synthesis by adenylyl cyclase (AC) and degradation by the cyclic nucleotide phosphodiesterase (PDE) enzymes. Until now, in vitro MAM can be achieved by increasing oocyte cAMP concentrations through the employment of several reagents, such as PDE inhibitors, AC activity stimulators, and cAMP analogs (Ramos Leal et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Combined use of dbcAMP and IBMX minimizes the damage induced by a long‐term artificial meiotic arrest in mouse germinal vesicle oocytes

Han

Hao

et al. 2020

Molecular Reproduction Devel

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Phosphodiesterase (PDE)‐mediated reduction of cyclic adenosine monophosphate (cAMP) activity can initiate germinal vesicle (GV) breakdown in mammalian oocytes. It is crucial to maintain oocytes at the GV stage for a long period to analyze meiotic resumption in vitro. Meiotic resumption can be reversibly inhibited in isolated oocytes by cAMP modulator forskolin, cAMP analog dibutyryl cAMP (dbcAMP), or PDE inhibitors, milrinone (Mil), Cilostazol (CLZ), and 3‐isobutyl‐1‐methylxanthine (IBMX). However, these chemicals negatively affect oocyte development and maturation when used independently. Here, we used ICR mice to develop a model that could maintain GV‐stage arrest with minimal toxic effects on subsequent oocyte and embryonic development. We identified optimal concentrations of forskolin, dbcAMP, Mil, CLZ, IBMX, and their combinations for inhibiting oocyte meiotic resumption. Adverse effects were assessed according to subsequent development potential, including meiotic resumption after washout, first polar body extrusion, early apoptosis, double‐strand DNA breaks, mitochondrial distribution, adenosine triphosphate levels, and embryonic development. Incubation with a combination of 50.0 μM dbcAMP and 10.0 μM IBMX efficiently inhibited meiotic resumption in GV‐stage oocytes, with low toxicity on subsequent oocyte maturation and embryonic development. This work proposes a novel method with reduced toxicity to effectively arrest and maintain mouse oocytes at the GV stage.

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“…On the other hand, GO terms significantly enriched by host genes generating DECs in oocytes are mainly related to oocyte meiosis, such as female meiotic division, cAMP signaling, and VEGF signaling. For instance, both WEE2 enriched in the female meiotic division and cAMP molecules have been shown to inhibit the resumption of oocyte meiosis in several species (Hanna et al, 2010; Ramos Leal et al, 2018). In contrast, VEGF supplement significantly improved maturation rate of oocytes in cattle (Luo et al, 2002), sheep (Yan et al, 2012) and pigs (Bui et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Circular RNA profiling in the oocyte and cumulus cells reveals thatcircARMC4is essential for porcine oocyte maturation

Cao

Gao

et al. 2019

Preprint

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Thousands of circular RNAs (circRNAs) have been recently discovered in cumulus cells and oocytes from several species. However, the expression and function of circRNA during porcine oocyte meiotic maturation have been never examined. Here, we separately identified 7,067 and 637 circRNAs in both the cumulus cell and the oocyte via deep sequencing and bioinformatic analysis. Further analysis revealed that a faction of circRNAs is differentially expressed (DE) in a developmental stage-specific manner. The host genes of DE circRNAs are markedly enriched to multiple signaling pathways associated with cumulus cell function and oocyte maturation. Additionally, most DE circRNAs harbor several miRNA targets, suggesting that these DE circRNAs potentially act as miRNA sponge. Importantly, we found that maternal circARMC4 knockdown by siRNA microinjection caused a severely impaired chromosome alignment, and significantly inhibited first polar body extrusion and early embryo development. Taken together, these results demonstrate for the first time that circRNAs are abundantly and dynamically expressed in a developmental stage-specific manner in cumulus cells and oocytes, and maternally expressed circARMC4 is essential for porcine oocyte meiotic maturation and early embryo development.

show abstract

Role of cAMP modulator supplementations during oocyte in vitro maturation in domestic animals

Cited by 32 publications

References 148 publications

Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte maturation

Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte maturation

Combined use of dbcAMP and IBMX minimizes the damage induced by a long‐term artificial meiotic arrest in mouse germinal vesicle oocytes

Circular RNA profiling in the oocyte and cumulus cells reveals thatcircARMC4is essential for porcine oocyte maturation

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