Effects of in Utero and Lactational Exposure to Bisphenol A on Somatic Growth and Anogenital Distance in F1 Rat Offspring.

Abstract

Bisphenol A (BPA), a xenoestrogen, has been reported to mimic the actions of estrogen or to affect the endocrine glands in vivo and in vitro. In this study, we examined whether in utero and lactational exposure to BPA altered the somatic growth and anogenital distance (AGD) of F 1 offspring (1, 3, and 9 weeks of age) in vivo in rats. Dams were orally administered with various doses of BPA (0, 4, or 40 mg/kg body weight (BW)/day) from gestation day (GD) 6 through postnatal day (PND) 20. There were no significant changes in body weight, liver weight, kidneys weight, testes weight, AGD, the ratio of AGD to BW, or the ratio of AGD to the cube root of BW in BPA exposed pups compared to the vehicle-exposed control. This suggests that prenatal and postnatal exposure (indirect exposure) to BPA (4-40 mg/kg/day, GD 6-PND 20) does not affect on somatic growth or AGD of F 1 generation of male and female rats.

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

“…The 400 mg/kg bw/day maternal group was excluded from analysis because of excessive maternal toxicity. Details about maternal toxicity and additional aspects of this study are available in the summary for the study by Kobayashi et al (2002). On PND 7, litters were culled to 5 pups/sex when possible.…”

“…Kobayashi et al (2002), supported by the Japanese Ministry of the Environment, examined the effect of prenatal and lactational bisphenol A exposure on somatic growth and anogenital distance in Sprague-Dawley rats. The same rats were used to measure plasma hormone levels and testicular testosterone content in a study by Watanabe et al (2003) and apparently thyroid function in a study by Koybayashi et al (2005).…”

Effects of in Utero and Lactational Exposure to Bisphenol A on Thyroid Status in F1 Rat Offspring

“…Table 1 shows the treatment design and number of subjects examined. The 400 mg/kg/day group was excluded from further analysis because of its excessive maternal toxicity 16) .…”

Imbalance of Testosterone Level in Male Offspring of Rats Perinatally Exposed to Bisphenol A

“…As stated in our previous report 12) , only one dam and its pups in the highest dose group survived after delivery, and these pups were only used in the sampling at 36 weeks old. Testes were dissected out and weighed.…”

“…The effects of BPA on the development and growth of the offspring have been presented in our earlier report 12) . No morphological abnormality was observed in the offspring in any of the BPA treatment groups, and the differences in the body weights at 1, 3 and 9 weeks old among groups were not significant.…”