Effects of trans‐2‐hexenal on reproduction, growth and behaviour and efficacy against the pinewood nematode, Bursaphelenchus xylophilus

Abstract

trans-2-Hexenal had significant nematicidal activity against B. xylophilus, providing a basis for elucidation of the mode of action of trans-2-hexenal against plant-parasitic nematodes in future studies. © 2016 Society of Chemical Industry.

Assessments of iodoindoles and abamectin as inducers of methuosis in pinewood nematode, Bursaphelenchus xylophilus

“…xylophilus with maximum inhibition at 50 µg/mL, although significant declines in nematode survival were noted at 10 and 20 µg/mL (Fig. 1E), which concurs with previous results 20 . Enhanced activity was observed when 5-iodoindole and abamectin were used in combination at 0.05 mM and 10 µg/mL respectively (~11 ± 5% survival) (Fig.…”

“…xylophilus . Inhibitory effect of nematicides on thrashing behaviors of PWN have been widely reported 20, 38–40 . Time-lapse imaging revealed 5-iodoindole and abamectin treated nematodes (Fig.…”

“…Adult nematodes (~500) were initially treated with 5-iodoindole (0.05 and 0.1 mM) or abamectin (10 µg/mL) and incubated for 24 h at 22 °C as described by Cheng et al ., 2016 20 . After 24 h, approximately, 10 female and male nematodes were randomly picked and placed at the center of B .…”

“…xylophilus movement in liquid culture is characterized by stereotypical flexing around the midpoint of the body 20 . Thrashing movements of synchronized juveniles (L2) were monitored by placing them in sterilized distilled water after 24 h treatments with 5-iodoindole (0.05 mM and 0.1 mM) or abamectin (10 µg/mL).…”

Screening, isolation and evaluation of a nematicidal compound from actinomycetes against the pine wood nematode, Bursaphelenchus xylophilus

“…Eggs were then incubated in M9 buffer (22 mM KH 2 PO 4 , 42 mM Na 2 HPO 4 , 86 mM NaCl, 1 mM MgSO 4 ) in the absence of food, at 25 °C. Two days later, the synchronised larvae were transferred to a Petri dish containing a fresh fungal food source ( B. cinerea ) and the adult nematodes were collected at 78 h. Approximately 500 adult nematodes that were a mixture of females and males were initially treated with the purified active compound (spectinabilin) at 0.21 (LC 10 ) and 0.48 µg mL −1 (LC 30 ) and incubated at 25 °C for 24 h, as described by Cheng et al . Approximately 24 h later, 10 male and female nematodes were picked randomly and placed on a potato dextrose agar plate, fully covered with B. cinerea , and allowed to grow until one of the plates was fully consumed.…”