Screening, isolation and evaluation of a nematicidal compound from actinomycetes against the pine wood nematode, <i>Bursaphelenchus xylophilus</i>

Liu, Min-Jiao; Hwang, Byung-Soon; Jin, Chun-Zhi; Li, Wenjun; Park, Dong-Jin; Seo, Sang‐Tae; Kim, Chang‐Jin

doi:10.1002/ps.5272

Cited by 45 publications

(45 citation statements)

References 46 publications

(83 reference statements)

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“…Biological options are gaining attention as promising new tools due to their environmentally-friendly and non-toxic characteristics. The potential for using microbes in controlling plant-parasitic nematodes has been documented [38] and effective microbes have been obtained from soil, plants, and the surface of nematodes [39][40][41]. Aspergillus spp.…”

Section: Discussionmentioning

confidence: 99%

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Wang

et al. 2020

Agronomy

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The root-knot nematode is one of the most damaging plant-parasitic nematodes worldwide, and the ecofriendly alternative approach of biological control has been used to suppress nematode populations. Here the nematicidal activity of Aspergillus japonicus ZW1 fermentation filtrate against Meloidogyne incognita was evaluated in vitro and in greenhouse, and the effects of A. japonicus ZW1 fermentation filtrate on seed germination and the active compound of A. japonicus ZW1 fermentation filtrate were determined. The 2-week fermentation filtrate (2-WF) of A. japonicus ZW1 exhibited markedly inhibitory effects on egg hatching, and 5% 2-WF showed potential nematicidal activities on second-stage juveniles (J2s); the mortality of J2s was 100% after 24 h exposure. The internal contents of nematodes were degraded and remarkable protruded wrinkles were present on the body surface of J2s. The nematicidal activity of the fermentation was stable after boiling and was not affected by storage time. A germination assay revealed that 2-WF did not have a negative effect on the viability and germination of corn, wheat, rice, cowpeas, cucumbers, soybeans, or tomato seeds. The pot-grown study confirmed that a 20% fermentation broth solution significantly reduced root galls and egg numbers on tomatoes, and decreased galls and eggs by 47.3% and 51.8% respectively, over Czapek medium and water controls. The active compound from the A. japonicus ZW1 fermentation filtrate was isolated and identified as 1,5-Dimethyl Citrate hydrochloride ester on the basis of nuclear magnetic resonance (NMR) and LC-MS (liquid chromatograph-mass spectrometer) techniques. Thus, fermentation of A. japonicus ZW1 could be considered a potential new biological nematicide for the control of M. incognita.

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Section: Discussionmentioning

confidence: 99%

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Wang

et al. 2020

Agronomy

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“…Taxa within Streptomyces have been shown in previous studies to be enriched in SCN cysts in long-term monoculture and SCN suppressive soils (Zhu et al, 2013; Hussain et al, 2018) and are well known for their production of secondary metabolite toxins or antibiotics. Some of these metabolites, including avermectins produced by the Actinomycete Streptomyces avermitilis (Cabrera et al, 2013) that are the active ingredient of the commercial nematicidal seed treatment Abamectin ® , as well as others compounds may serve as nematode toxins or in antagonism of egg hatching (Liu et al, 2019). LEfSe analysis also identified OTUs within the genus Haliangium in the Myxococcales (Deltaproteobacteria) or “slime bacteria” to be enriched in cysts from soybean crop sequences in fall of both years (Figures 5, 6, Table 6, and Supplementary Table S6).…”

Section: Discussionmentioning

confidence: 99%

Seasonal Variation and Crop Sequences Shape the Structure of Bacterial Communities in Cysts of Soybean Cyst Nematode

Strom

Haarith

et al. 2019

Front. Microbiol.

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Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is the number 1 pathogen of the important economic crop soybean. Bacteria represent potential biocontrol agents of the SCN, but few studies have characterized the dynamics of bacterial communities associated with cysts under different crop rotation sequences. The bacterial communities in SCN cysts in a long-term soybean–corn crop rotation experiment were investigated over 2 years. The crop sequences included long-term soybean monoculture (Ss), years 1–5 of soybean following 5 years corn (S1–S5), years 1 and 2 of corn following 5 years soybean (C1 and C2), and soybean–corn annual rotation (Sa and Ca). The bacterial 16S rRNA V4 region was amplified from DNA isolated from SCN cysts collected in spring at planting, midseason (2 months later), and fall at harvest and sequenced on the Illumina MiSeq platform. The SCN cyst microbiome was dominated by Proteobacteria followed by Actinobacteria, Bacteroidetes, and Verrucomicrobia. The bacterial community composition was influenced by both crop sequence and season. Although differences by crop sequence were not significant in the spring of each year, bacterial communities in cysts from annual rotation (Sa and Ca) or crop sequences of early years of monoculture following a 5-year rotation of the alternate crop (S1 and C1) became rapidly differentiated by crop over a single growing season. In the fall, genera of cyst bacteria associated with soybean crop sequences included Rhizobacter, Leptothrix, Cytophaga, Chitinophaga, Niastella, Streptomyces, and Halangium. The discovery of diverse bacterial taxa in SCN cysts and their dynamics across crop rotation sequences provides invaluable information for future development of biological control of the SCN.

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“…The nematicidal activity of endophytic actinomycetes isolated from Korean pines was determined. A total of 170 endophytic actinomycetes were incubated at 28 °C for 7 days in Bennett's agar medium 24 (10 g glucose, 1 g yeast extract, 1 g beef extract, 2 g peptone and 1 L DW), followed by 7 days in a GSS broth medium 25 (20 g glucose, 10 g soluble starch, 25 g soybean meal, 2 g NaCl, 0.25 g K 2 HPO 4 , 2 g CaCO 3 , 1 g beef extract, 4 g yeast extract and 1 L DW). Next, 500 μL of each culture broth was extracted with 500 μL of acetone and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

Nematicidal activity of teleocidin B4 isolated from Streptomyces sp. against pine wood nematode, Bursaphelenchus xylophilus

Kang

Kim

Liu

et al. 2021

Pest Management Science

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BACKGROUND Pine wilt disease (PWD) is a plant disease that causes serious damage to pine trees. PWD occurs when the host plant is infected with pinewood nematode (PWN), Bursaphelenchus xylophilus. In this study, a compound with nematicidal activity was isolated from actinomycetes and its efficacy was investigated in vitro. RESULT We screened and selected Streptomyces sp. 680560, which had nematicidal activity against B. xylophilus. Based on 16S rRNA sequence analysis, it showed 99.93% similarity with Streptomyces blastmyceticus NRRB‐5480T. Furthermore, the active compound was isolated and identified as teleocidin B4. Teleocidin B4 at concentrations ranging from 6.25 to 100 μM had low nematicidal activity after 24 and 36 h against adult and stage juveniles (J2) of B. xylophilus, but after 48 h nematicidal activity exceeded 95%. The rate of inhibition of egg hatching for Teleocidin B4 6.25, 12.5, 25, 50, and 100 μM was confirmed to be dose‐dependently inhibited after 48 h of treatment. Teleocidin B4 is not only toxic to hatched B. xylophilus, but also affects egg hatching. CONCLUSION This study was carried out to isolate actinomycete metabolites from pine tree endophytes from various natural environments for control of PWD. A compound with nematicidal activity was isolated from a selected strain and its structure was identified as teleocidin B4. The nematicidal effect of the isolated active substance, teleocidin B4, was confirmed. This is the first report of the effect of teleocidin B4 on B. xylophilus, suggesting its possibility as a PWD control agent. © 2020 Society of Chemical Industry

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Screening, isolation and evaluation of a nematicidal compound from actinomycetes against the pine wood nematode, Bursaphelenchus xylophilus

Cited by 45 publications

References 46 publications

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Seasonal Variation and Crop Sequences Shape the Structure of Bacterial Communities in Cysts of Soybean Cyst Nematode

Nematicidal activity of teleocidin B4 isolated from Streptomyces sp. against pine wood nematode, Bursaphelenchus xylophilus

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