Effects of <i>Pinus massoniana</i> pollen polysaccharides on intestinal microenvironment and colitis in mice

Niu, Xiangyun; Shang, Hongqi; Chen, Ruichang; Huang, Jin; Miao, Yongqiang; Cui, Wenping; Wang, Huan; Zhou, Sha; Peng, Duo; Zhu, Rui‐Liang

doi:10.1039/d0fo02190c

Cited by 23 publications

(10 citation statements)

References 66 publications

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“…Dysfunctional gut microbial structure affects physiological processes such as energy metabolism, immune regulation and liver injury in humans (76)(77)(78). Niu et al evaluated the effect of Pinus massoniana pollen polysaccharides (PPPS) on the intestinal flora of mice by 16S rRNA high-throughput sequencing technology and showed that PPPS can regulate the composition of mouse intestinal microorganisms and increase the proportion of probiotic bacteria, and also regulate the systemic immune system by modulating the immunosuppressive status of lymphocytes in Peyer's patches (79). N. sativa seed polysaccharides can exert immune modulatory effects by improving the structure of the intestinal flora, increasing flora diversity, and regulating metabolic pathways such as lipid metabolism, polysaccharide synthesis and signal transduction (59).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

et al. 2022

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Proteomics and intestinal flora were used to determine the mechanism of immune modulatory effects of Flammulina velutipes polysaccharide on immunosuppressed mice. The results showed that compared with the model group, F. velutipes polysaccharide could increase thymus and spleen indices and improve thymus tissue structure in mice; IL-2 and IL-4 contents were significantly increased and IL-6 and TNF-α contents were significantly decreased; serum acid phosphatase (ACP), lactate dehydrogenase (LDH) and total antioxidant capacity (T-AOC) activities were increased (P < 0.05); in the liver, superoxide dismutase (SOD) and catalase (CAT) activities were increased (P < 0.001), while malondialdehyde (MDA) content was decreased (P < 0.001). Proteomics discovered that F. velutipes polysaccharides may exert immune modulatory effects by participating in signaling pathways such as immune diseases, transport and catabolism, phagosomes and influenza A, regulating the immune-related proteins Transferrin receptor protein 1 (TFRC) and Radical S-adenosyl methionine domain-containing protein 2 (RSAD2), etc. Gut microbial studies showed that F. velutipes polysaccharides could increase the abundance of intestinal flora and improve the flora structure. Compared to the model group, the content of short-chain fatty acids (SCFAs) and the relative abundance of SCFA-producers Bacteroides and Alloprevotella were increased in the F. velutipes polysaccharide administration group, while Lachnospiraceae_NK4A136_group and f_Lachnospiraceae_Unclassified decreased in relative abundance. Thus, F. velutipes polysaccharide may play an immunomodulatory role by regulating the intestinal environment and improving the balance of flora.

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Section: Discussionmentioning

confidence: 99%

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

et al. 2022

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“…NO was considered as an important mediator in the inflammatory response produced by the stimulation of inducible nitric oxide synthase (iNOS). The pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in intestinal epithelial cells played a crucial role in intestinal inflammation, which were accumulated in inflammation and tissue damage ( Niu et al, 2021 ). TNF-α was reported to induce intestinal barrier damage and inflammation of IEC-6 cells ( Zhuang et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells

et al. 2021

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“…[27] Oral administration of high doses of FMPS effectively up-regulated the number of Lachnospiraceae_NK4A136_group, which was considered to be an important butyrate producer and could maintain immune homeostasis by regulating the immune balance of Th17/Treg. [28,29] In addition, FMPS could significantly up-regulate the level of SCFAs. As essential molecular mediators between intestinal microbes and the immune system, SC-FAs can directly or indirectly regulate the differentiation of Treg cells to maintain intestinal homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium‐Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota‐Dependent Manner

Zou

Wang

Liu

et al. 2022

Molecular Nutrition Food Res

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Scope Ulcerative colitis (UC) is a common chronic recurrent inflammatory bowel disease. This study attempts to reveal the improvement mechanism of floral mushroom polysaccharide (FMPS) on UC from the perspective of coordinated interaction between intestinal microbes and intestinal helper T cell 17 (Th17)/regulatory T cell (Treg) balance. Methods and results Dextran sulfate sodium (DSS)‐induced colitis mice model is used for the experiment. The results suggest that FMPS up‐regulated the expression of occludin, ZO‐1, and MUC2, and down‐regulated the secretion of TNF‐α, IL‐1β, and IL‐6 in colitis mice. Importantly, FMPS restores intestinal Th17/Treg balance. Meanwhile, FMPS can regulate intestinal microorganisms and improve the level of short‐chain fatty acids (SCFAs) in colitis mice. Intestinal microbial depletion and fecal microbiota transplantation (FMT) experiments reveal that FMPS ameliorated UC is mediated by intestinal microbiome. Flow cytometry further proves that FMPS restores intestinal Th17/Treg balance in a microbial‐dependent manner. Conclusion These results indicate that FMPS has the potential to improve UC, and its mechanism depends on the restoration of Th17/Treg balance mediated by intestinal microorganisms. Therefore, it is suggested that FMPS dietary supplement can be potentially used to intervene UC.

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Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice

Abstract: The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine.

Cited by 23 publications

References 66 publications

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells

Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium‐Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota‐Dependent Manner

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