Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells

Xiong, Lixia; Gong, Yufeng; Yao, Wanzi; Chen, Xiaoyong; Xian, Jiebei; You, Lijun; Fardim, Pedro

doi:10.1016/j.fochx.2021.100157

Cited by 13 publications

(5 citation statements)

References 41 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of the seaweed resources have not been exploited and utilized, resulting in a serious waste problem. Seaweed contains a variety of bioactive components, of which polysaccharides have attracted particular attention because of their diverse health-promoting functions such as anti-inflammatory [ 2 ], anti-cancer [ 3 , 4 ], immunomodulatory [ 5 ], and gut microbiota regulatory [ 6 , 7 , 8 , 9 , 10 ] effects. However, seaweed polysaccharides have deficiencies such as a large molecular weight, poor solubility, and low bioavailability, limiting their application [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Yao

Jiang

et al. 2023

Marine Drugs

Self Cite

View full text Add to dashboard Cite

The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H2O2) treatment was used to prepare low-molecular-weight seaweed polysaccharides from Sargassum fusiforme. The effects of UV/H2O2 treatment on the physicochemical properties and anti-photoaging activity of S. fusiforme polysaccharides were studied. UV/H2O2 treatment effectively degraded polysaccharides from S. fusiforme (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of S. fusiforme polysaccharides were investigated using human epidermal HaCaT cells in vitro. DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H2O2 treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H2O2 treatment could effectively improve the anti-photoaging activity of S. fusiforme polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Yao

Jiang

et al. 2023

Marine Drugs

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent research has demonstrated that a wide range of active ingredients identified in algae can combat cancer, viruses, and bacteria; reduce inflammatory responses; and halt angiogenesis [3][4][5]. The properties of algae polysaccharides differ from those of their naturally existing equivalents in that they are abundant in sulfated and uronic acid residues [6][7][8]. Ulvan primarily comprises a series of monosaccharides: uronic acid, iduronic acid, rhamnose sulfate, or xylose [9].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the algal polysaccharides are beneficial due to their low toxicity, strong biocompatibility, and immunoregulatory properties [6]. Marine-sulfated polysaccharides may be viable options to combat and treat the SARS-CoV-2 infection.…”

Section: Introductionmentioning

confidence: 99%

Prospective Antiviral Effect of Ulva lactuca Aqueous Extract against COVID-19 Infection

Binsuwaidan,

El-Masry,

El-Sheekh

et al. 2023

Marine Drugs

View full text Add to dashboard Cite

Marine algal extracts exhibit a potent inhibitory effect against several enveloped and non-enveloped viruses. The infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has several adverse effects, including an increased mortality rate. The anti-COVID-19 agents are still limited; this issue requires exploring novel, effective anti-SARS-CoV-2 therapeutic approaches. This study investigated the antiviral activity of an aqueous extract of Ulva lactuca, which was collected from the Gulf of Suez, Egypt. The aqueous extract of Ulva lactuca was characterized by high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and Energy Dispersive X-ray (EDX) analyses. According to the HPLC analysis, the extract comprises several sugars, mostly rhamnose (32.88%). The FTIR spectra showed numerous bands related to the functional groups. EDX analysis confirmed the presence of different elements, such as oxygen (O), carbon (C), sulfur (S), magnesium (Mg), potassium (K), calcium (Ca), and sodium (Na), with different concentrations. The aqueous extract of U. lactuca (0.0312 mg/mL) exhibited potent anti-SARS-CoV-2 activity via virucidal activity, inhibition of viral replication, and interference with viral adsorption (% inhibitions of 64%, 33.3%, and 31.1%, respectively). Consequently, ulvan could be a promising compound for preclinical study in the drug development process to combat SARS-CoV-2.

show abstract

“…Special attention has been paid to food ingredients like polysaccharides, with potential activity to maintain intestinal barrier integrity, especially plant polysaccharides, due to their negligible safety risks and widespread existence in our daily diets. For example, the polysaccharides from Portulaca oleracea L. could maintain barrier function in the porcine intestinal epithelial cell monolayer via activating the EGF/EGFR signaling pathway [5], while those from Gracilaria lemaneiformis might enhance intestinal barrier function and maintain intestinal health [10]. Additionally, the polysaccharides from Tremella fuciformis and Dendrobium huoshanense can restore intestinal and mucosal barrier function [11,12].…”

Section: Introductionmentioning

confidence: 99%

Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells

Yu,

Zhao

2023

Nutrients

View full text Add to dashboard Cite

In this study, the soluble, but non-digestible, longan (Dimocarpus longan Lour.) polysaccharides (LP) were extracted from dried longan fruits and then chemically selenylated to produce two selenylated products, namely SeLP1 and SeLP2, with different selenylation extents. The aim was to investigate their protective effects on rat intestinal epithelial (IEC-6) cells exposed to the food toxin fumonisin B1 (FB1). LP only contained total Se content of less than 0.01 g/kg, while SeLP1 and SeLP2 were measured with respective total Se content of up to 1.46 and 4.79 g/kg. The cell viability results showed that these two selenylated products were more efficient than LP in the IEC-6 cells in alleviating FB1-induced cell toxicity, suppressing lactate dehydrogenase (LDH) release, and decreasing the generation of intracellular reactive oxygen species (ROS). These two selenylated products were also more effective than LP in combating FB1-induced barrier disruption via increasing the transepithelial electric resistance (TEER), reducing the paracellular permeability, decreasing the mitochondrial membrane potential (MMP) loss, and maintaining cell barrier integrity by upregulating the tight-junction-related genes and proteins. FB1 caused cell oxidative stress and barrier dysfunction by activating the MAPK and mitochondrial apoptosis signaling pathways, while SeLP1 and SeLP2 could regulate the tMAPK- and apoptosis-related proteins to suppress the FB1-mediated activation of the two pathways. Overall, SeLP2 was observed to be more active than SeLP1 in the IEC-6 cells. In conclusion, the chemical selenylation of LP caused an activity enhancement to ameliorate the FB1-induced cell cytotoxicity and intestinal barrier disruption. Meanwhile, the increased selenylation of LP would endow the selenylated product SeLP2 with more activity.

show abstract

Structural characterization and protective effects of polysaccharide from Gracilaria lemaneiformis on LPS-induced injury in IEC-6 cells

Cited by 13 publications

References 41 publications

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Prospective Antiviral Effect of Ulva lactuca Aqueous Extract against COVID-19 Infection

Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells

Contact Info

Product

Resources

About