Effects of Eriobotrya japonica Water Extract on Alcoholic and Nonalcoholic Fatty Liver Impairment

Abstract

The aim of this study was to investigate the potential protective effects of the hot water extract of Eriobotrya japonica (EJW) on EtOH-or free fatty acid (FFA)-induced fatty liver injury in vitro. HepG2/2E1 cells were exposed to EtOH and HepG2 cells were exposed to a mixture of FFAs (oleic acid:palmitic acid, 2:1) to stimulate oxidative stress and to induce lipid accumulation, respectively. Antioxidant activity was significantly increased and lipid accumulation was inhibited in cells pretreated with EJW compared to those in cells exposed to EtOH or FFA only. Also, 5 0 adenosine monophosphate (AMP)activated protein kinase (AMPK) and acetyl-coenzyme A carboxylase (ACC) phosphorylations were considerably increased, indicating activation of AMPK. Furthermore, EJW reduced the messenger RNA (mRNA) expression of lipogenesis-associated factors such as ACC, sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FAS), and increased mRNA expression related to components of the fatty acid b-oxidation pathway, such as AMPK, carnitine palmitoyltransferase 1 (CPT-1), and peroxisome proliferator-activated receptor alpha (PPARa). These results suggest that EJW possessed potential preventive effects against both EtOH-and FFA-induced fatty liver disease by alleviation of oxidative stress and lipid accumulation in hepatocytes.

Hepatoprotective Activity and Mechanisms of Prenylated Stilbenoids

“…Based on this finding, we hypothesized that these compounds may alleviate hepatic steatosis by activating the SIRT1-PGC1α pathway. Our results were consistent with previous reports showing that inactivation of the SIRT1-PGC1α signaling pathway occurs in lipotoxicity. − However, treatment with 4- C -geranyl oxyresveratrol upregulated genes in the SIRT1-PGC1α signaling pathway in our study. These findings suggested that the hepatoprotective activity by 4- C -geranyl oxyresveratrol in palmitic acid-treated cells could be at least partially due to the activation of the SIRT1-PGC1α pathway.…”

Forkhead box protein O1 (FoxO1) regulates lipids metabolism and cell proliferation mediated by insulin and PI3K-Akt-mTOR pathway in goose primary hepatocytes

“…When the content of TG produced far exceeded the transport capacity of apolipoproteins, and the fatty acid produced far exceeded the degraded fatty acid by β-oxidation, the accumulation of lipids occurred (Mun et al, 2019;Wei et al, 2021). FoxO1 is a critical regulator of hepatocyte lipid deposition, FoxO1 can regulate hepatic lipid metabolism from the lipid lipogenesis, fatty acids oxidation and lipids transportation (Liu et al, 2019).…”

Eriobotrya japonica Fermentation with Plant-Derived Lactiplantibacillus plantarum MSC-5T Ameliorates Antioxidant Activity in HEK293 Cells

“…An ethanolic extract of 80 µg/mL concentration increased cell viability by 36% in HEPG2 cells exposed to 200 mM ethanol [20]. Another example where extract yield and cell viability effects have been noted is for hot water extract of EJ leaves (100 µg/mL) with a hepatoprotective effect at close to 90% in ethanol-treated HEPG2 cells [21]. Nevertheless, from a cell toxicity standpoint in the HEK293 cell line in the present study, what perhaps is more important is the observed cell protection response with unfermented EJ extract after H 2 O 2 exposure (Figure 1b).…”