Effects ofEnterococcus faecalissonicated extracts on IL-2, IL-4 and TGF-β1 production from human lymphocytes

Abstract

In order to examine the immunoresponse of host cells to Enterococcus faecalis, this in vitro study monitored the production of Interleukin-2 (IL-2), Interleukin-4 (IL-4) and Transforming growth factor-β 1 (TGF-β 1) in human lymphocytes. Lymphocytes were activated with PHA in the presence or abscence of sonicated extracts of E. Faecalis (SEF) and further incubated for 72 hours. The level of each cytokine was measured by ELISA. Data were analyzed with Kruskal-Wallis test and MannWhitney U test (P < 0.05). PHA-activated group did exhibit higher level of IL-2 and IL-4 than untreated control group. The levels of expression of both cytokines were significantly decreased following the treatment of high (25 ㎍/㎖) and medium concentration (12.5 ㎍/㎖) of SEF (P <0 .05) than those of PHA activated group. But low concentration (5 ㎍/㎖) of SEF showed the similar level of IL-2 and IL-4 production as those of PHA activated group. TGF-β 1 was unaffected by SEF treatment. These results suggested that E. faecalis may suppress IL-2 and IL-4 production by lymphocytes and this could be one of possible factors why E. faecalis are found frequently in the teeth with failed endodontic treatment. [J Kor Acad Cons Dent 30(1): [1][2][3][4][5][6] 2005]

Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls.In vitroSEM study

“… 16 , 17 Kim et al suggested the ability of the E. faecalis to suppress IL-2 and IL-4 production by lymphocytes to be one of the factors. 18 C. albicans is usually associated with bacteria, especially E. faecalis . 19 The selection of these pathogens as test organisms was because of their clinical importance and extensive use in previous studies.…”