Role(s) of cytokines in pulpitis: Latest evidence and therapeutic approaches

Khorasani, M.; Hassanshahi, Gholamhossein; Brodzikowska, Aniela; Khorramdelazad, Hossein

doi:10.1016/j.cyto.2019.154896

Cited by 45 publications

(39 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22,72,98 Dental pulp inflammation is an adaptive immune process with changes in local blood flow, neuronal activity, and immunocompetent cell function. 5,44,117 Numerous studies have indicated that iNOS-derived NO, COX2-derived prostaglandin E2 (PGE 2 ) , histamine, substance P, neuropeptides, IL-1b, IL-6, IL-8, and TNF-a are major inflammatory mediators that are involved in one or more of these processes in pulp inflammation, [118][119][120] while NO may be involved in all steps of the initiation and/or progression of pulpitis. 8,72,118 Some evidences for this statement may be gleaned from the following researches.…”

Section: Anmentioning

confidence: 99%

“…11,156 IL-1 is a typical multifunctional cytokine involved in immune responses, hematopoiesis, and inflammation with regard to both innate and adaptive immunity 157 ; it is also a predominant cytokine in pulpal inflammation. 120 The most salient property of IL-1 (mainly IL-1a and IL-1b) is its ability to activate iNOS and COX2, which accounts for the production of NO and PGE 2 . 158 HO-1 possesses a cytoprotective effect against oxidative damage and inflammatory responses and thus may contribute to good clinical outcomes through a sequence of healing events.…”

Section: The No System As a Potential Therapeutic Target For Dental Pmentioning

confidence: 99%

See 1 more Smart Citation

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

2020

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

Nitric oxide (NO), which is synthesized by the enzyme NO synthase (NOS), is a versatile endogenous molecule with multiple biological effects on many tissues and organs. In dental pulp tissue, NO has been found to play multifaceted roles in regulating physiological activities, inflammation processes, and tissue repair events, such as cell proliferation, neuronal degeneration, angiogenesis, and odontoblastic differentiation. However, there is a deficiency of detailed discussion on the NO-mediated interactions between inflammation and reparative/ regenerative responses in wounded dental pulp tissue, which is a central determinant of ultimate clinical outcomes. Thus, the purpose of this review is to outline the current molecular understanding on the roles of Janusfaced molecule NO in dental pulp physiology, inflammation, and reparative activities. Based on this knowledge, advanced physicochemical techniques designed to manipulate the therapeutic potential of NOS and NO production in endodontic regeneration procedures are further discussed.

show abstract

Section: Anmentioning

confidence: 99%

Section: The No System As a Potential Therapeutic Target For Dental Pmentioning

confidence: 99%

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

2020

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

show abstract

“…Dental pulp is not isolated in the oral cavity and releases many biological products to the external environment in response to external harmful stimuli [ 12 – 14 ]. At the cellular or molecular level, a wide range of molecules are released during pulpal and periapical inflammation, including cytokines, proteases, inflammatory mediators, growth factors, and antimicrobial peptides [ 15 , 16 ]. Measurable levels of molecules can be found not only in pulp tissue but also in pulpal blood [ 17 ], dentinal fluid [ 18 , 19 ], periapical fluid [ 1 ], and gingival crevicular fluid [ 20 ], which can be collected non-invasively and analysed without extirpating the pulpal tissue [ 19 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic biomarker candidates for pulpitis revealed by bioinformatics analysis of merged microarray gene expression datasets

et al. 2020

View full text Add to dashboard Cite

Background Pulpitis is an inflammatory disease, the grade of which is classified according to the level of inflammation. Traditional methods of evaluating the status of dental pulp tissue in clinical practice have limitations. The rapid and accurate diagnosis of pulpitis is essential for determining the appropriate treatment. By integrating different datasets from the Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix of pulpitis, aiming to identify biological pathways and diagnostic biomarkers of pulpitis. Methods By integrating two datasets (GSE77459 and GSE92681) in the GEO database using the sva and limma packages of R, differentially expressed genes (DEGs) of pulpitis were identified. Then, the DEGs were analysed to identify biological pathways of dental pulp inflammation with Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and Gene Set Enrichment Analysis (GSEA). Protein–protein interaction (PPI) networks and modules were constructed to identify hub genes with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and Cytoscape. Results A total of 470 DEGs comprising 394 upregulated and 76 downregulated genes were found in pulpitis tissue. GO analysis revealed that the DEGs were enriched in biological processes related to inflammation, and the enriched pathways in the KEGG pathway analysis were cytokine-cytokine receptor interaction, chemokine signalling pathway and NF-κB signalling pathway. The GSEA results provided further functional annotations, including complement system, IL6/JAK/STAT3 signalling pathway and inflammatory response pathways. According to the degrees of nodes in the PPI network, 10 hub genes were identified, and 8 diagnostic biomarker candidates were screened: PTPRC, CD86, CCL2, IL6, TLR8, MMP9, CXCL8 and ICAM1. Conclusions With bioinformatics analysis of merged datasets, biomarker candidates of pulpitis were screened and the findings may be as reference to develop a new method of pulpitis diagnosis.

show abstract

“…At the cellular or molecular level, wide ranges of molecules are released in pulpal and periapical inflammation, including cytokines, proteases, inflammatory mediators, growth factors, antimicrobial peptides and so on [10,11]. The factors that can used to judge the level of the pulp inflammation are called biomarkers of pulpitis [12].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic biomarker candidates for pulpitis revealed by bioinformatics analysis of merged microarray gene expression datasets

Chen

Zeng

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Pulpitis is known as an inflammatory disease classified by the level of inflammation. The existed traditional methods of evaluating status of dental pulp tissue in clinical practice still have some shortages and limitations. Immediate and accurate diagnosis of pulpitis is essential to the choice of treatment. Through integrating different datasets from Gene Expression Omnibus (GEO) database, we analyzed the merged expression matrix of pulpitis, aiming to identified biological pathways and diagnostic biomarker of pulpitis.Methods By integrating two datasets (GSE77459 and GSE92681) in GEO database using sva and limma packages, differentially expressed genes (DEGs) of pulpitis were identified. Then DEGs were used to analyze biological pathways of dental pulp inflammation with Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and the Gene Set Enrichment Analysis (GSEA). Protein–protein interaction (PPI) networks and modules were constructed to identify hub genes with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and Cytoscape.Results A total of 472 DEGs consisting of 396 upregulated and 76 downregulated genes were found in pulpitis tissue. DEGs in GO analysis were enriched in biological processes about inflammation and in KEGG pathway analysis were cytokine-cytokine receptor interaction, chemokine signaling pathway and NF-κB signaling pathway. GSEA results provided further functional annotations including complement system, IL6/JAK/STAT3 signaling pathway and inflammatory response pathways. According to the degrees of nodes in PPI network, 10 hub genes were obtained and 8 diagnostic biomarker candidates were screened, including PTPRC, CD86, CCL2, IL6, TLR8, MMP9, CXCL8 and ICAM1.

show abstract

Role(s) of cytokines in pulpitis: Latest evidence and therapeutic approaches

Cited by 45 publications

References 112 publications

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

Diagnostic biomarker candidates for pulpitis revealed by bioinformatics analysis of merged microarray gene expression datasets

Diagnostic biomarker candidates for pulpitis revealed by bioinformatics analysis of merged microarray gene expression datasets

Contact Info

Product

Resources

About