Effects of<i>Bacillus subtilis</i>on Epithelial Tight Junctions of Mice with Inflammatory Bowel Disease

Gong, Yi; Li, Hui; Li, Yan

doi:10.1089/jir.2015.0030

Cited by 53 publications

(39 citation statements)

References 40 publications

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“…4). A recent study [55] has demonstrated the ability of a Bacillus probiotic to upregulate the expression of TJ proteins in colitic mice, in line with these observations with B. coagulans supplementation. In our previous study, B. coagulans spores in synbiotic combination with sugar cane fibre showed similar beneficial effects on the epithelial barrier function in DSSinduced colitic mice [19].…”

Section: Discussionsupporting

confidence: 61%

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases

et al. 2020

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Purpose The research goal is to develop dietary strategies to help address the growing incidence of inflammatory bowel diseases (IBD). This study has investigated the effectiveness of green banana resistant starch (GBRS) and probiotic Bacillus coagulans MTCC5856 spores for the amelioration of dextran-sulfate sodium (DSS)-induced colitis in mice. Methods Eight-week-old C57BL/6 mice were fed standard rodent chow diet supplemented with either B. coagulans, GBRS or its synbiotic combination. After 7 days supplementation, colitis was induced by adding 2% DSS in drinking water for 7 days while continuing the supplemented diets. Animal health was monitored and after 14 days all animals were sacrificed to measure the biochemical and histochemical changes associated with each supplement type. Results The disease activity index and histological damage score for DSS-control mice (6.1, 17.1, respectively) were significantly higher (p < 0.0001) than the healthy mice. Synbiotic supplementation alleviated these markers (− 67%, − 94% respectively) more adequately than B. coagulans (− 52%, − 58% respectively) or GBRS (− 57%, − 26%, respectively) alone. Compared to DSS-control synbiotic supplementation significantly (p < 0.0001) maintained expressions of tight junction proteins. Moreover, synbiotic effects accounted for ~ 40% suppression of IL-1β and ~ 29% increase in IL-10 levels in serum while also reducing C-reactive protein (− 37%) compared to that of the DSS-control. While, B. coagulans alone could not induce additional levels of short-chain fatty acid (SCFA) production beyond the caecum, the synbiotic combination with GBRS resulted in substantial increased SCFA levels across the whole length of the colon. Conclusion The synbiotic supplementation with B. coagulans and GBRS ameliorated the overall inflammatory status of the experimental IBD model via synergistic functioning. This supports researching its application in mitigating inflammation in human IBD.

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Section: Discussionsupporting

confidence: 61%

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases

et al. 2020

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“…40,41 In an IBD mouse model, decreased ZO-1 abundance and elevated intestinal permeability were found to occur ahead of obvious colonic inflammation, suggesting a critical role for ZO-1 in the intestinal barrier. 42 We observed decreased intestinal ZO-1 expression associated with the depletion of F. prausnitzii in DM mice. MAM from F. prausnitzii potentially interacts with the tight junction pathway.…”

Section: Discussionmentioning

confidence: 61%

“…The aberrant expression of ZO‐1 may hinder the formation of intercellular tight junctions and impair the intestinal mechanical barrier, allowing pathogens and bacterial toxins to enter the bloodstream and leading to systematic complications such as intestinal infections, systemic inflammatory response syndrome, and multiple organ failure . In an IBD mouse model, decreased ZO‐1 abundance and elevated intestinal permeability were found to occur ahead of obvious colonic inflammation, suggesting a critical role for ZO‐1 in the intestinal barrier . We observed decreased intestinal ZO‐1 expression associated with the depletion of F. prausnitzii in DM mice.…”

Section: Discussionmentioning

confidence: 73%

Faecalibacterium prausnitzii‐derived microbial anti‐inflammatory molecule regulates intestinal integrity in diabetes mellitus mice via modulating tight junction protein expression

Liang

Zhang

et al. 2019

Journal of Diabetes

111

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Background Impaired intestinal barrier structure and function have been validated as an important pathogenic process in type 2 diabetes mellitus (T2DM). Gut dysbiosis is thought to be the critical factor in diabetic intestinal pathogenesis. As the most abundant commensal bacteria, Faecalibacterium prausnitzii (F. prausnitzii) play important roles in gut homeostasis. The microbial anti‐inflammatory molecule (MAM), an F. prausnitzii metabolite, has anti‐inflammatory potential in inflammatory bowel disease (IBD). Thus, we aimed to explore the function and mechanism of MAM on the diabetic intestinal epithelium. Methods 16S high‐throughput sequencing was used to analyze the gut microbiota of db/db mice (T2DM mouse model). We transfected a FLAG‐tagged MAM plasmid into human colonic cells to explore the protein‐protein interactions and observe cell monolayer permeability. For in vivo experiments, db/db mice were supplemented with recombinant His‐tagged MAM protein from E. coli BL21 (DE3). Results The abundance of F. prausnitzii was downregulated in the gut microbiota of db/db mice. Immunoprecipitation (IP) and mass spectroscopy (MS) analyses revealed that MAM potentially interacts with proteins in the tight junction pathway, including zona occludens 1 (ZO‐1). FLAG‐tagged MAM plasmid transfection stabilized the cell permeability and increased ZO‐1 expression in NCM460, Caco2, and HT‐29 cells. The db/db mice supplemented with recombinant His‐tagged MAM protein showed restored intestinal barrier function and elevated ZO‐1 expression. Conclusions Our study shows that MAM from F. prausnitzii can restore the intestinal barrier structure and function in DM conditions via the regulation of the tight junction pathway and ZO‐1 expression.

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“…Significant enrichment of the core node Haemophilus was observed in infants with PBB, and it was negatively correlated with other microbial colonizers, including Lactococcus, Bacillus and Clostridium. Previous reports have shown that Bacillus affect the secretion of IL-17 indirectly (27), and Clostridium could promote T regulatory (Treg) cell differentiation, which were crucial for inflammation reactions (22,28). In infants with PBB, the relative abundances of aforementioned genera decreased as Haemophilus increased, and this was found to aggravate microbial imbalance and expand the immune response in the respiratory mucosa.…”

Section: Discussionmentioning

confidence: 93%

Bronchoalveolar lavage fluid microbiota dysbiosis in infants with protracted bacterial bronchitis

Bao

Li²,

Qiu³

et al. 2018

J. Thorac. Dis.

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Background: Protracted bacterial bronchitis (PBB) is a chronic purulent bronchitis which could cause recurrent coughing and wheezing in infants. Based on previous reports, main pathogens which caused PBB were identified in the patients, but their impacts on lung microbiota dysbiosis remain unclear.Methods: In this study, bronchoalveolar lavage fluid (BALF) was collected from PBB infants and tracheomalacia (TM) infants younger than 3 years old under the instruction of Shenzhen Children's Hospital, and 12 samples were randomly selected for 16S rDNA analysis in each group. Based on the results of bacterial composition, the microbiota diversity and co-occurrence network in PBB and TM group were detected and compared. Results: Microbiota diversity was significantly lower in PBB group than it in TM group (P<0.001 for the comparison of Shannon and Simpson indexes). The PBB group was found to harbor 25 accumulated bacterial agents by comparison with TM group, including Haemophilus (P<0.001) and Bacteroides (P<0.001). Whilst, the populations of Lactococcus (P<0.001) and Lactobacillus (P<0.001) were dramatically smaller in PBB group.The co-occurrence network in PBB group also differed from that of TM group. It contained four core nodes in PBB patients, including Haemophilus, Parabacteroides, Porphyromonas, and Cronobacter. Haemophilus was found to be negatively associated with most counterparts, including Clostridium and Bacillus. Conclusions: PBB infants contained discrepant lung genera and co-occurrence network when compared with TM infants. This retrospective study may deepen our understanding of PBB pathogenesis, and it also provided a foundation for bacterial adjunctive therapy of infantile PBB in accordance with clinical treatment.

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Effects ofBacillus subtilison Epithelial Tight Junctions of Mice with Inflammatory Bowel Disease

Cited by 53 publications

References 40 publications

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases

Faecalibacterium prausnitzii‐derived microbial anti‐inflammatory molecule regulates intestinal integrity in diabetes mellitus mice via modulating tight junction protein expression

Bronchoalveolar lavage fluid microbiota dysbiosis in infants with protracted bacterial bronchitis

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