Effects ofAngelica gigasNakai as an Anti-Inflammatory Agent inIn VitroandIn VivoAtopic Dermatitis Models

Abstract

We investigated the cellular and molecular mechanisms mediating the effects of Angelica gigas Nakai extract (AGNE) through the mitogen-activated protein kinases (MAPKs)/NF-κB pathway using in vitro and in vivo atopic dermatitis (AD) models. We examined the effects of AGNE on the expression of proinflammatory cytokines and chemokines in human mast cell line-1 (HMC-1) cells. Compound 48/80-induced pruritus and 2,4-dinitrochlorobenzene- (DNCB-) induced AD-like skin lesion mouse models were also used to investigate the antiallergic effects of AGNE. AGNE reduced histamine secretion, production of proinflammatory cytokines including interleukin- (IL-) 1β, IL-4, IL-6, IL-8, and IL-10, and expression of cyclooxygenase- (COX-) 2 in HMC-1 cells. Scratching behavior and DNCB-induced AD-like skin lesions were also attenuated by AGNE administration through the reduction of serum IgE, histamine, tumor necrosis factor-α (TNF-α), IL-6 levels, and COX-2 expression in skin tissue from mouse models. Furthermore, these inhibitory effects were mediated by the blockade of the MAPKs and NF-κB pathway. The findings of this study proved that AGNE improves the scratching behavior and atopy symptoms and reduces the activity of various atopy-related mediators in HMC-1 cells and mice model. These results suggest the AGNE has a therapeutic potential in anti-AD.

Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines

“…Since a large number of herbal medicines and their secondary metabolites display anti-inflammatory effects [12,13,28,31], they have been traditionally used as remedies and currently utilized as complementary medicines [31,32]. Among medicinal plants, the AGN extract was verified to have excellent anti-inflammatory effects.…”

“…Among medicinal plants, the AGN extract was verified to have excellent anti-inflammatory effects. The ethanol extract of AGN, for instance, displayed anti-inflammatory effects by reducing the production of histamine and atopy-related cytokines and suppressing the levels of COX-2, NF-κB, and Iκ-Bα in both in vivo and in vitro models of atopic dermatitis [12]. Additionally, AGN extract prevented epithelial injury in colonic tissues of a murine model of dextran sulfate sodium-induced ulcerative colitis by moderating the activation of inflammatory mediators, such as IL-6, TNF-α, prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2) [33].…”

“…Angelica gigas Nakai (AGN) belongs to the Umbelliferae family and has been traditionally used in oriental remedies due to its many advantageous attributes, such as antiepileptic, hepatoprotective, anti-cancer, anti-inflammatory, and anti-amnesic activities [12][13][14][15][16][17]. Concerning the case of hair growth using AGN, the extract of the roots of AGN is frequently included as a hair growth-facilitating component in combined herbal extracts [18][19][20][21].…”

Network Pharmacology-Based Investigation of the System-Level Molecular Mechanisms of the Hematopoietic Activity of Samul-Tang, a Traditional Korean Herbal Formula

“…We retrieved the chemical compounds present in the four herbal medicines that constitute SMT (i.e., AGR, RRP, PRA, and CR) from traditional Chinese medicine (TCM)-related databases, including the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database, Traditional Chinese Medicine Integrated Database (TCMID), and Herb Ingredients' Targets (HIT) database [49][50][51][52]. Regarding AGR, we first explored the biochemical constituents of Angelicae Sinensis Radix, a commonly used herbal medicine in China that belongs to the Angelicae species, using the aforementioned TCM-related databases, and combined those with the reported major compounds of AGR such as marmesin, lomatin, and decursin [53][54][55]. Chemical compounds not contained in AGR, including isoeugenol, stigmasterol, and 4-octanone, were excluded from the integrated data based on previous studies [53][54][55].…”

“…Regarding AGR, we first explored the biochemical constituents of Angelicae Sinensis Radix, a commonly used herbal medicine in China that belongs to the Angelicae species, using the aforementioned TCM-related databases, and combined those with the reported major compounds of AGR such as marmesin, lomatin, and decursin [53][54][55]. Chemical compounds not contained in AGR, including isoeugenol, stigmasterol, and 4-octanone, were excluded from the integrated data based on previous studies [53][54][55].…”