Effects of hypoxia on cardiomyocyte proliferation and association with stage of development

Sun, Yanjun; Jiang, Chuan; Hong, Haifa; Liu, Jinfen; Qiu, Lisheng; Huang, Yanhui; Ye, Lincai

doi:10.1016/j.biopha.2019.109391

Cited by 24 publications

(20 citation statements)

References 26 publications

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“…However, significantly less nuclear-localized YAP was seen in cells exposed to hypoxia or a magnetic field alone without generation of torque. Interestingly, hypoxic conditions activate nuclear localization of YAP, which leads to various effects, such as cell proliferation and growth [44,45]. In our experiment, we observed a significant increase in nuclear localization of YAP when torque was generated under hypoxia, while, for hypoxia without torque, nuclear localization of YAP was not observed in cardiomyocytes and fibroblasts.…”

Section: Discussionmentioning

confidence: 43%

Development of Magnetic Torque Stimulation (MTS) Utilizing Rotating Uniform Magnetic Field for Mechanical Activation of Cardiac Cells

et al. 2020

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Regulation of cell signaling through physical stimulation is an emerging topic in biomedicine. Background: While recent advances in biophysical technologies show capabilities for spatiotemporal stimulation, interfacing those tools with biological systems for intact signal transfer and noncontact stimulation remains challenging. Here, we describe the use of a magnetic torque stimulation (MTS) system combined with engineered magnetic particles to apply forces on the surface of individual cells. MTS utilizes an externally rotating magnetic field to induce a spin on magnetic particles and generate torsional force to stimulate mechanotransduction pathways in two types of human heart cells—cardiomyocytes and cardiac fibroblasts. Methods: The MTS system operates in a noncontact mode with two magnets separated (60 mm) from each other and generates a torque of up to 15 pN µm across the entire area of a 35-mm cell culture dish. The MTS system can mechanically stimulate both types of human heart cells, inducing maturation and hypertrophy. Results: Our findings show that application of the MTS system under hypoxic conditions induces not only nuclear localization of mechanoresponsive YAP proteins in human heart cells but also overexpression of hypertrophy markers, including β-myosin heavy chain (βMHC), cardiotrophin-1 (CT-1), microRNA-21 (miR-21), and transforming growth factor beta-1 (TGFβ-1). Conclusions: These results have important implications for the applicability of the MTS system to diverse in vitro studies that require remote and noninvasive mechanical regulation.

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Section: Discussionmentioning

confidence: 43%

Development of Magnetic Torque Stimulation (MTS) Utilizing Rotating Uniform Magnetic Field for Mechanical Activation of Cardiac Cells

et al. 2020

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“…However, neonatal rat ventricular myocytes (NRVMs), when compared to adult rat ventricular myocytes (ARVMs), had the advantage of being isolated in high yield and purity by the enzymatic dissociation method [23]. Furthermore, since NRVMs are immature compared to ARVMs, these cells are actively used as a major cell source for studies including cardiac maturation [31][32][33], development [34][35][36], disease modeling [37,38], and drug screening [39,40].…”

Section: Cardiomyocytesmentioning

confidence: 99%

Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering

et al. 2021

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Cardiac tissue engineering aims to generate in vivo-like functional tissue for the study of cardiac development, homeostasis, and regeneration. Since the heart is composed of various types of cells and extracellular matrix with a specific microenvironment, the fabrication of cardiac tissue in vitro requires integrating technologies of cardiac cells, biomaterials, fabrication, and computational modeling to model the complexity of heart tissue. Here, we review the recent progress of engineering techniques from simple to complex for fabricating matured cardiac tissue in vitro. Advancements in cardiomyocytes, extracellular matrix, geometry, and computational modeling will be discussed based on a technology perspective and their use for preparation of functional cardiac tissue. Since the heart is a very complex system at multiscale levels, an understanding of each technique and their interactions would be highly beneficial to the development of a fully functional heart in cardiac tissue engineering.

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“…A population of hypoxic cardiomyocytes in transgenic mice improved CM proliferation in the adult heart, suggesting that hypoxic cell signalling plays an impor-tant role in CM cell cycling [31]. It is possible that induction of CM proliferation may be based on developmental stage of cells [30,32,33]. The changing effects of hypoxia on CMs across developmental stages may be linked to changes in structure and composition from embryo to adult [33].…”

Section: Hypoxiamentioning

confidence: 99%

“…It is possible that induction of CM proliferation may be based on developmental stage of cells [30,32,33]. The changing effects of hypoxia on CMs across developmental stages may be linked to changes in structure and composition from embryo to adult [33]. By reducing presence of reactive oxygen species, hypoxia may benefit CM proliferation [32].…”

Section: Hypoxiamentioning

confidence: 99%

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Cardiac Stem Cell Therapy, Resident Progenitor Cells and the role of Cellular Signalling; a Review

Hutchings

Nawrocki

Mozdziak

et al. 2019

Medical Journal of Cell Biology

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Cardiovascular disease (CVD) remains the most common cause of death worldwide. Unhealthy lifestyle choices promote an upward trend of primary risk factors for CVD. As a result, novel methods of treatment are required. The myocardium itself could serve as a source of treatment, via resident cardiac progenitor cells (CPC). A brief overview of current studies and findings related to the potential of differentiation of CPCs to form mature cardiomyocytes (CM) and thereby heal damaged myocardial tissue, as well as implications of these findings for further research areas and possible treatments, is offered. Also investigated is the possible role of CM cell reprogramming, cardiac fibroblasts and signalling molecules in treatment of CVD. Running title: Cardiac stem cells -review

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Effects of hypoxia on cardiomyocyte proliferation and association with stage of development

Cited by 24 publications

References 26 publications

Development of Magnetic Torque Stimulation (MTS) Utilizing Rotating Uniform Magnetic Field for Mechanical Activation of Cardiac Cells

Development of Magnetic Torque Stimulation (MTS) Utilizing Rotating Uniform Magnetic Field for Mechanical Activation of Cardiac Cells

Recapitulating Cardiac Structure and Function In Vitro from Simple to Complex Engineering

Cardiac Stem Cell Therapy, Resident Progenitor Cells and the role of Cellular Signalling; a Review

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