Effects of hyperbaric oxygen on Leishmania amazonensis promastigotes and amastigotes

Arrais-Silva, Wagner Welber; Collhone, Marcelle Carolina; Ayres, Diana Copi; Souto, Paula Cristina de Souza; Giorgio, Selma

doi:10.1016/j.parint.2004.07.002

Cited by 23 publications

(24 citation statements)

References 25 publications

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“…Recently, we showed that HBO is toxic for L. amazonensis promastigotes and can reduce macrophage susceptibility to infection and the size of footpad lesion in BALB/c mice 2,3 . In the present study, the inhibition of the infection in macrophages treated with glucantime in combination with HBO was greater than that of macrophages treated with glucantime alone and HBO alone.…”

Section: Discussionmentioning

confidence: 99%

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

Ayres

Fedele

Marcucci

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIn this study we investigated the efficacy of hyperbaric oxygen (HBO) therapy, alone or combined with the pentavalent antimonial glucantime on Leishmania amazonensis infection. In parallel, the effect of Brazilian red propolis gel (propain) alone or combined with glucantime on L. amazonensis infection was evaluated. The inhibition of the infection in macrophages treated with glucantime in combination with HBO exposition was greater than that of macrophages treated with glucantime alone or HBO alone. The susceptible mouse strain BALB/c infected in the shaved rump with L. amazonensis treated with glucantime and exposed to HBO showed: time points in the course of the disease in which lesions were smaller than those of mice treated with glucantime alone and revascularization of the skin in the lesion site; interferon-gamma (IFN-γ) levels were not elevated in lymph node cells from these animals. Propain alone was not efficient against lesions, although less exudative lesions were observed in animals treated with propain alone or combined with glucantime. These results reveal the potential value of HBO and red propolis in combination with glucantime for treating cutaneous leishmaniasis and encourage further studies on the effect of more aggressive HBO, propolis and glucantime therapies on different mouse models of leishmaniasis.

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Section: Discussionmentioning

confidence: 99%

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

Ayres

Fedele

Marcucci

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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“…5A). In experiments with lesion-derived (Lemesre et al 1997, Arrais-Silva et al 2005. Addition of 25 µg/ml BRV in medium did not affect amastigotes differentiation to promastigotes (48 h) (Fig.…”

Section: Resultsmentioning

confidence: 85%

<![CDATA[<B>Effects of Brazilian propolis on <I>Leishmania amazonensis</i></B>]]>

Ayres¹,

Marcucci²,

Giorgio³

2007

Mem. Inst. Oswaldo Cruz

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“…Observations drawn from human studies suggest that HBO might be useful in the treatment of some bacterial and fungal infections, like purpura fulminans (89) and necrotizing fasciitis (90). However, only a few studies have investigated the application of HBO to protozoan infections (91,92), including ECM (93,94).…”

Section: Hyperbaric Oxygenmentioning

confidence: 99%

Pathophysiological Mechanisms in Gaseous Therapies for Severe Malaria

et al. 2016

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cOver 200 million people worldwide suffer from malaria every year, a disease that causes 584,000 deaths annually. In recent years, significant improvements have been achieved on the treatment of severe malaria, with intravenous artesunate proving superior to quinine. However, mortality remains high, at 8% in children and 15% in adults in clinical trials, and even worse in the case of cerebral malaria (18% and 30%, respectively). Moreover, some individuals who do not succumb to severe malaria present longterm cognitive deficits. These observations indicate that strategies focused only on parasite killing fail to prevent neurological complications and deaths associated with severe malaria, possibly because clinical complications are associated in part with a cerebrovascular dysfunction. Consequently, different adjunctive therapies aimed at modulating malaria pathophysiological processes are currently being tested. However, none of these therapies has shown unequivocal evidence in improving patient clinical status. Recently, key studies have shown that gaseous therapies based mainly on nitric oxide (NO), carbon monoxide (CO), and hyperbaric (pressurized) oxygen (HBO) alter vascular endothelium dysfunction and modulate the host immune response to infection. Considering gaseous administration as a promising adjunctive treatment against severe malaria cases, we review here the pathophysiological mechanisms and the immunological aspects of such therapies. Malaria exerts a heavy burden over human populations, with an estimated 124 to 283 million cases and 584,000 deaths in 2013 (1). Currently, intravenous (i.v.) artesunate is the treatment of choice in severe malaria cases in children and adults (2, 3). However, despite the efficacy of intravenous artesunate, mortality from severe malaria in general and from cerebral malaria (CM) in particular remains high, at 18% for African children and 30% for adults in Southeast Asia (2, 3). In addition, 11% of children who survive CM show severe neurological deficits, and up to 25% can maintain long-term cognitive deficits (4-8). Therefore, strategies focusing only on parasite killing may not be sufficient to prevent neurological complications and deaths related to severe malaria. Accordingly, adjunctive therapies-defined as therapies administered in combination with antiparasitic drugs that modify pathophysiological processes caused by malaria-are being sought in order to mitigate complications caused by severe malaria (9). Considering the fact that currently administered antimalarial drugs often take 12 to 18 h to kill parasites, adjunctive therapies could reduce the risk of neurocognitive sequelae and mortality, particularly in patients with CM (10).Different adjunctive therapies have been or are being tested, including treatments aimed at modulation of the immune response to infection (dexamethasone, intravenous immunoglobulin), reduction of iron burden, reduction of oxidative stress, modulation of the prothrombotic state, and reduction of parasitemia (blood transfusion), amon...

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Effects of hyperbaric oxygen on Leishmania amazonensis promastigotes and amastigotes

Cited by 23 publications

References 25 publications

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

<![CDATA[<B>Effects of Brazilian propolis on <I>Leishmania amazonensis</i></B>]]>

Pathophysiological Mechanisms in Gaseous Therapies for Severe Malaria

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