Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa

Duru, Namık Kemal; Morshedi, Mahmood; Oehninger, Sergio

doi:10.1016/s0015-0282(00)01591-0

Cited by 241 publications

(74 citation statements)

References 22 publications

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“…Oxygen radicals at the physiological concentration have a positive effect, for example, on the intracellular signaling involved in the processes of cell proliferation, differentiation, and migration, as well as in sperm capacitation, hyperactivation, and sperm-oocyte fusion (14,15). Substantial evidence exists that small amounts of ROS are necessary for spermatozoa to acquire fertilizing capability (16,17).…”

Section: Ros Production By Defective and Decaying Spermatozoamentioning

confidence: 99%

Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species

Petruška¹,

Capcarová²,

Šutovský

2014

Turk J Vet Anim Sci

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Improving sire reproductive performance in artificial insemination (AI) programs and maximizing the use of sires with valuable genomes are high on the list of priorities for livestock breeders in the United States and worldwide. While fixed-time AI and accurate estrous detection have already ameliorated the reproductive management of cows and sows, substantial gains remain to be made in the selection and utilization of male animals for field AI. In addition to better, more objective evaluation of breeding soundness and semen quality, numbers of spermatozoa per AI dose could be reduced and numbers of AI doses per collection could be increased without sacrificing conception rates if the fittest spermatozoa can be purified from raw semen and protected from adverse effects of dying and decaying spermatozoa present in both the fresh and the extended semen. The present article reviews recent progress in methodology for semen purification, including nanopurification and semen/extender supplementation with antioxidants.

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Section: Ros Production By Defective and Decaying Spermatozoamentioning

confidence: 99%

Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species

Petruška¹,

Capcarová²,

Šutovský

2014

Turk J Vet Anim Sci

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“…A numbers of studies have suggested that the presence of spermatozoa with damaged DNA may be the result of an impaired chromatin packing or may be indicative of apoptosis (30, 31). Protamines may act as protective elements by sequestering metals capable of promoting the fragmentation of sperm DNA (32).…”

Section: Discussionmentioning

confidence: 99%

“…There are reports indicating that any disorder which resulted in a failure in epididymal sperm maturation, causes impaired sperm fertilizing ability (31, 33, 34). Development of spermatozoal ability to expose forward motility, undergoing capacitation, and penetrate the zona pellucida of the oocyte is examples of the several important properties, which the spermatozoa acquire during epididymal sperm passage (1).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats

Ebadimanas

2018

Comp Clin Pathol

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Background: Pyridaben, a pyridazinone derivative, is a new acaricide and insecticide for control of mites and some insects such as white flies, aphids and thrips. Objective: This study was designed to elucidate how pyridaben can affect the sperms' morphological parameters, its DNA integrity, and to estimate the effect of various quantities of pyridaben on in vitro fertilization rate. Materials and Methods: In this study, 80 adult male Balb/C strain mice were used. Animals were divided into control and two test groups. Control group received distilled water. The test group was divided into two subgroups, viz, high dose (212 mg/kg/day) and low dose (53 mg/kg/day) and they received the pyridaben, orally for duration of 45 days. The spermatozoa were obtained from caudae epididymides on day 45 in all groups. Sperm viability, protamin compression (nuclear maturity), DNA double-strand breaks, and in vitro fertilizing (IVF) ability were examined. Results: The pyridaben treatment provoked a significant decrease in sperm population and viability in epididymides. The data obtained from this experiment revealed that, the pyridaben brings about negative impact on the sperm maturation and DNA integrity in a time-dependent manner, which consequently caused a significant (p<0.05) reduction in IVF capability. Embryo developing arrest was significantly (p<0.05) higher in treated than the control group. Conclusion: Theses results confirmed that, the pyridaben is able to induce DNA damage and chromatin abnormalities in spermatozoa which were evident by low IVF rate.This article extracted from Ph.D. thesis. (Ghodrat Ebadi Mans)

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“…Oxidative damage to the sperm membranes leads to impaired cell-egg interactions, reduced ejaculate quality, and subfertility or infertility (Aitken, 1999;Aitken and Baker, 2006;Tremellen, 2008). Additionally, ROS attacks on sperm DNA can cause chromosome rearrangements, histone modifications, deletions, base modifications or changes in methylation patterns (Aitken and Krausz, 2001;Kemal Duru et al, 2000;Lloyd et al, 1997;Wellejus et al, 2000;Zitzmann et al, 2003), which renders sperm be unable to fertilize the ovum. For the above-mentioned reasons, OS is considered as one of the main causes of male infertility (Aitken and Baker, 2004;Tremellen, 2008).…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress affects sperm performance and ejaculate redox status in subordinate House Sparrows

Mora

Firth

Blareau

et al. 2017

Journal of Experimental Biology

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Oxidative stress is the result of random cellular damage caused by reactive oxygen species that leads to cell death, ageing or illness. Most physiological processes can result in oxidative stress, which in turn has been identified as a major cause of infertility. In promiscuous species, the fertilizing ability of the ejaculate partly determines the male reproductive success. When dominance determines access to fertile females, theory predicts that lower ranking males should increase resource investment into enhancing ejaculate quality. We hypothesized that subordinate males should thus prioritize antioxidant protection of their ejaculates to protect them from oxidative stress. We put this hypothesis to the test by chronically dosing wild house sparrows with diquat (∼1 mg kg), a herbicide that increases pro-oxidant generation. We found that, although they increased their antioxidant levels in the ejaculate, diquat-treated males produced sperm with reduced velocity. Importantly, and contrary to our hypothesis, males at the bottom of the hierarchy suffered the largest reduction in sperm velocity. We suggest that resource access hinders individuals' ability to cope with environmental hazards. Our results point at oxidative stress as a likely physiological mechanism mediating ejaculate quality, while individual ability to access resources may play a role in constraining the extent to which such resources can be allocated into the ejaculate.

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Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa

Cited by 241 publications

References 22 publications

Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species

Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species

Protective effect of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats

Oxidative stress affects sperm performance and ejaculate redox status in subordinate House Sparrows

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