Background: Pyridaben, a pyridazinone derivative, is a new acaricide and insecticide for control of mites and some insects such as white flies, aphids and thrips. Objective: This study was designed to elucidate how pyridaben can affect the sperms' morphological parameters, its DNA integrity, and to estimate the effect of various quantities of pyridaben on in vitro fertilization rate. Materials and Methods: In this study, 80 adult male Balb/C strain mice were used. Animals were divided into control and two test groups. Control group received distilled water. The test group was divided into two subgroups, viz, high dose (212 mg/kg/day) and low dose (53 mg/kg/day) and they received the pyridaben, orally for duration of 45 days. The spermatozoa were obtained from caudae epididymides on day 45 in all groups. Sperm viability, protamin compression (nuclear maturity), DNA double-strand breaks, and in vitro fertilizing (IVF) ability were examined. Results: The pyridaben treatment provoked a significant decrease in sperm population and viability in epididymides. The data obtained from this experiment revealed that, the pyridaben brings about negative impact on the sperm maturation and DNA integrity in a time-dependent manner, which consequently caused a significant (p<0.05) reduction in IVF capability. Embryo developing arrest was significantly (p<0.05) higher in treated than the control group. Conclusion: Theses results confirmed that, the pyridaben is able to induce DNA damage and chromatin abnormalities in spermatozoa which were evident by low IVF rate.This article extracted from Ph.D. thesis. (Ghodrat Ebadi Mans)
Background: Diazinon is an insecticide with oxidative stress properties, which is widely used in agriculture. Selenium is used as a basic nutrient for animals and humans as a potential antioxidant. No studies have evaluated the protective effects of selenium on diazinon in the reproductive system of male Wistar rats. Objectives: The present study aimed to assess the therapeutic effects of selenium on balanced testosterone levels, sperm DNA damage, fertilization, and early embryonic development in diazinon-treated rats. Methods: This experimental study was conducted on 32 mature male Wistar rats, which were randomly assigned to four groups of eight, including control (0.2 mg/kg/B.W of corn oil), diazinon (300 mg/kg/B.W), selenium (0.5 mg of Na2SeO3/kg), selenium (0.5 mg/kg), and diazinon (300 mg/kg/B.W of diazinon). The animals received daily oral treatment for 60 days. Results: Compared to the control group, the diazinon group showed a significant reduction in the testosterone levels, sperm count, sperm motility, sperm maturation, DNA maturity, in-vitro fertilization (IVF), two-cell embryo, hatching, and blastocyst (P value < 0.05). However, the sperm mortality, sperm DNA damage, and immature sperm significantly increased in the treatment group (P value < 0.05). In the diazinon and selenium treatment group, the total sperm parameters returned to the physiological range, and no significant changes were observed. Conclusions: According to the results, selenium could protect the sperm parameters, testosterone level, and IVF in the diazinon-treated Wistar rats.
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