Effects of human collagen α-1 type I-derived proteins on collagen synthesis and elastin production in human dermal fibroblasts

Hwang, Su Jin; Kim, Su Hwan; Seo, Wontaek; Jeong, Yoseok; Shin, Min Cheol; Ryu, Dongryeol; Lee, Sang Bae; Choi, Young Jin; Kim, KyeongJin

doi:10.5483/bmbrep.2021.54.6.038

Cited by 16 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TGF-β1 stimulated increase of both gene and protein levels of type I collagen and fibronectin in both fibroblast types and of type III collagen in pulmonary fibroblasts correlates with previous findings in SSc, fibrotic pulmonary, and healthy dermal and pulmonary fibroblasts 22 – 26 , 28 , 29 , 45 , 46 . The increase of FN1 and FBN-C, PRO-C1 and PRO-C6 in both fibroblast types, and PRO-C3 in dermal fibroblasts by PDGF-AB stimulation is consistent with previous findings 28 , 41 , 47 .…”

Section: Discussionsupporting

confidence: 89%

Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts’ response to fibrotic growth factors

Madsen

Sand

Juhl

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Systemic Sclerosis (SSc) hallmark is skin fibrosis, but up to 80% of the patients have fibrotic involvement in the pulmonary system. Antifibrotic drugs which have failed in a general SSc population have now been approved in patients with SSc-associated interstitial lung disease (ILD). This indicates that the fibrotic progression and regulation of fibroblasts likely depend on local factors specific to the tissue type. This study investigated the difference between dermal and pulmonary fibroblasts in a fibrotic setting, mimicking the extracellular matrix. Primary healthy fibroblasts were grown in a crowded environment and stimulated with TGF-β1 and PDGF-AB. The viability, morphology, migration capacity, extracellular matrix formation, and gene expression were assessed: TGF-β1 only increased the viability in the dermal fibroblasts. PDGF-AB increased the migration capacity of dermal fibroblasts while the pulmonary fibroblasts fully migrated. The morphology of the fibroblasts was different without stimulation. TGF-β1 increased the formation of type III collagen in pulmonary fibroblasts, while PDGF-AB increased it in dermal fibroblasts. The gene expression trend of type VI collagen was the opposite after PDGF-AB stimulation. The fibroblasts exhibit different response profiles to TGF-β1 and PDGF-AB; this suggests that drivers of fibrosis are tissue-dependent, which needs to be considered in drug development.

show abstract

Section: Discussionsupporting

confidence: 89%

Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts’ response to fibrotic growth factors

Madsen

Sand

Juhl

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…There are various types of collagen, and the dermis is mainly composed of 80%–85% collagen type I and 10%–15% collagen type III. Collagen type I is a trimer mainly composed of two α1 chains and one α2 chain, encoded by the COL1A1 and COL1A2 genes, respectively 14,15 . Both Col1a1 and Col1a2 mRNA expression levels were significantly decreased in the skin of the SAMP mice compared with the SAMR mice (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…Collagen type I is a trimer mainly composed of two α1 chains and one α2 chain, encoded by the COL1A1 and COL1A2 genes, respectively. 14,15 Both Col1a1 and Col1a2 mRNA expression levels were significantly decreased in the skin of the SAMP mice compared with the SAMR mice (Fig. 2d).…”

Section: Expression Levels Of Elastin Filaggrin Loricrin and Collagen...mentioning

confidence: 91%

Expression analysis of genes important for maintaining skin function in a senescence‐accelerated mouse prone model

Shinozaki,

Ikarashi,

Tabata

et al. 2023

Geriatrics Gerontology Int

View full text Add to dashboard Cite

AimSenescence‐accelerated mouse prone (SAMP) mice can reproduce the same conditions as normal aging mice in a short period. Although SAMP mice have been widely used in aging research, research on skin function in SAMP mice is lacking. In this study, to investigate the skin function of SAMP mice, we analyzed the expression of genes important for maintaining skin function.MethodsEight‐month‐old SAMP mice and senescence‐accelerated mouse resistant (SAMR) mice with normal aging were used. The expression levels of various functional genes in the skin were analyzed.ResultsThe dermal water content of SAMP mice was significantly lower than that of SAMR mice, indicating dry skin. The mRNA expression levels of elastin (Ela), filaggrin (Flg), loricrin (Lor), collagen type I alpha 1 chain (Col1a1) and Col1a2 in the skin of SAMP mice were all significantly decreased compared with those of SAMR mice. Hyaluronan‐degrading enzyme (Hyal1) expression levels in SAMP mice were similar to those in SAMR mice, but hyaluronan synthase (Has2) levels were significantly decreased. In addition, the expression level of aquaporin‐3 in the skin of SAMP mice was significantly decreased at both the mRNA and protein levels.ConclusionsIn the skin of SAMP mice, the expression levels of various skin function‐regulating genes were decreased, and this phenomenon might cause skin dryness. The SAMP mouse could be a tool for analyzing skin aging. Geriatr Gerontol Int 2023; ••: ••–••.

show abstract

“…The reported results show that collagen hydrolysate, derived from bovine type I collagen, notably modulated cell metabolism, increasing the content of type I procollagen and collagen by stimulating their biosynthesis and inhibiting MMP-1 and MMP-2 on both chronological aged and photoaged HDF. In Hwang et al's [51,52] studies, human alpha-1 and alpha-2 type I collagen (COL1A1 and COL1A2)-derived peptides revealed stimulatory effects on type I collagen and elastin production on HDF. Both COL1A1 and COL1A2-derived peptides exerted the same effect promoting collagen synthesis and elastin production.…”

Section: Discussionmentioning

confidence: 99%

A Novel Hyaluronic Acid Matrix Ingredient with Regenerative, Anti-Aging and Antioxidant Capacity

Gálvez‐Martín

Fernández

Romero‐Rueda

et al. 2023

IJMS

View full text Add to dashboard Cite

Hyaluronic acid (HA) and proteoglycans (such as dermatan sulphate (DS) and chondroitin sulphate (CS)) are the main components of the extracellular matrix of the skin, along with collagen and elastin. These components decrease with age, which implies a loss of skin moisture causing wrinkles, sagging and aging. Currently, the external and internal administration of effective ingredients that can reach the epidermis and dermis is the main alternative for combating skin aging. The objective of this work was to extract, characterise and evaluate the potential of an HA matrix ingredient to support anti-aging. The HA matrix was isolated and purified from rooster comb and characterised physicochemically and molecularly. In addition, its regenerative, anti-aging and antioxidant potential and intestinal absorption were evaluated. The results show that the HA matrix is composed of 67% HA, with an average molecular weight of 1.3 MDa; 12% sulphated glycosaminoglycans, including DS and CS; 17% protein, including collagen (10.4%); and water. The in vitro evaluation of the HA matrix’s biological activity showed regenerative properties in both fibroblasts and keratinocytes, as well as moisturising, anti-aging and antioxidant effects. Furthermore, the results suggest that the HA matrix could be absorbed in the intestine, implying a potential oral as well as topical use for skin care, either as an ingredient in a nutraceutical or a cosmetic product.

show abstract

Effects of human collagen α-1 type I-derived proteins on collagen synthesis and elastin production in human dermal fibroblasts

Cited by 16 publications

References 30 publications

Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts’ response to fibrotic growth factors

Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts’ response to fibrotic growth factors

Expression analysis of genes important for maintaining skin function in a senescence‐accelerated mouse prone model

A Novel Hyaluronic Acid Matrix Ingredient with Regenerative, Anti-Aging and Antioxidant Capacity

Contact Info

Product

Resources

About