Effects of hirsutine, an antihypertensive indole alkaloid from Uncaria rhynchophylla, on intracellular calcium in rat thoracic aorta

Horie, Syunji; Yano, Shingo; Aimi, Norio; Sakai, Shin‐ichiro; Watanabe, Kazuo

doi:10.1016/0024-3205(92)90388-6

Cited by 70 publications

(34 citation statements)

References 23 publications

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“…10,11) Its vasodilatory effects are mediated primarily via release of endothelium derived relaxing factor/nitric oxide (EDRF/NO) and inhibition of voltage-dependent Ca 2ϩ influx. [12][13][14] Uncaria rhynchophylla also inhibits tumor necrosis factor-a (TNF-a) and nitric oxide production in BV-2 mouse microglial cells in vitro. The neuroprotective effects of Uncaria rhynchophylla following transient global ischemia have also been evaluated.…”

Section: -7)mentioning

confidence: 99%

Corynoxeine Isolated from the Hook of Uncaria rhynchophylla Inhibits Rat Aortic Vascular Smooth Muscle Cell Proliferation through the Blocking of Extracellular Signal Regulated Kinase 1/2 Phosphorylation

Kim

Lee

et al. 2008

Biological & Pharmaceutical Bulletin

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The proliferation of vascular smooth muscle cells (VSMCs) induced by injury to the intima of arteries is an important etiologic factor in vascular proliferative disorders such as atherosclerosis and restenosis. Uncaria rhynchophylla is traditional Chinese herb that has been applied to the treatment of convulsive disorders, such as epilepsy, in China. In the present study, we examined whether corynoxeine exerts inhibitory effects on plateletderived growth factor (PDGF)-BB-induced rat aortic VSMC proliferation and the possible mechanism of such effects. Pre-treatment of VSMCs with corynoxeine (5-50 m mM) for 24 h resulted in significant decreases in cell number without any cytotoxicity; the inhibition percentages were 25.0؎12.5, 63.0؎27.5 and 88.0؎12.5% at 5, 20 and 50 m mM, respectively. Also, corynoxeine significantly inhibited the 50 ng/ml PDGF-BB-induced DNA synthesis of VSMCs in a concentration-dependent manner without any cytotoxicity; the inhibitions were 32.8؎11.0, 51.8؎8.0 and 76.9؎7.4% at concentrations of 5, 20 and 50 m mM, respectively. Pre-incubation of VSMCs with corynoxeine significantly inhibited PDGF-BB-induced extracellular signal-regulated kinase 1/2 (ERK1/2) activation, whereas corynoxeine had no effects on mitogen-activated protein kinase (MAPK/ERK)-activating kinase 1 and 2 (MEK1/2), Akt, or phospholipase C (PLC)g g1 activation or on PDGF receptor beta (PDGF-Rb b) phosphorylation. These results suggest that corynoxeine is a potent ERK1/2 inhibitor of key PDGF-BB-induced VSMC proliferation and may be useful in the prevention and treatment of vascular diseases and restenosis after angioplasty.

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Section: -7)mentioning

confidence: 99%

Corynoxeine Isolated from the Hook of Uncaria rhynchophylla Inhibits Rat Aortic Vascular Smooth Muscle Cell Proliferation through the Blocking of Extracellular Signal Regulated Kinase 1/2 Phosphorylation

Kim

Lee

et al. 2008

Biological & Pharmaceutical Bulletin

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“…Uncaria species belonging to Rubiaceae are known to contain corynanthe and heteroyohimbine type indole and oxindole alkaloids. 7 Some corynanthe and oxindole type alkaloids such as geissoschizine methyl ether, 8 hirsutine, 9 and rhynchophylline 10 showed vasorelaxant effects against isolated strips of rat aorta. Uncaria species have been used in traditional medicine by the native people of the Chinese for the treatment of antihypertensive agents.…”

Section: Introductionmentioning

confidence: 99%

New vasorelaxant indole alkaloids, villocarines A–D from Uncaria villosa

Matsuo

Okamoto

Zaima

et al. 2011

Bioorganic & Medicinal Chemistry

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Villocarines A-D (1-4), four new indole alkaloids have been isolated from the leaves of Uncaria villosa (Rubiaceae) and their structures were elucidated by 2D NMR methods and chemical correlations. Villocarine A (1) showed vasorelaxation activity against rat aortic ring and showed inhibition effect on vasocontraction of depolarized aorta with high concentration potassium, and also inhibition effect on phenylephrine (PE)-induced contraction in the presence of nicardipine in a Ca(2+) concentration-dependent manner. The vasorelaxant effect by 1 might be attributed mainly to inhibition of calcium influx from extracellular space through voltage-dependent calcium channels (VDC) and/or receptor-operated Ca(2+)-channels (ROC), and also partly mediated through the increased release of NO from endothelial cells and opening of voltage-gated K(+)-channels.

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“…Several modes of hypotensive action have been suggested such as, antagonism of Ca 2+ channel, through α-adrenoceptor or direct relaxant effects on myocardium or blood vessels. Peripheral modes involving antagonism of Ca 2+ have been suggested to be the mediator of observed hypotensive action in case of plants such as, Annona muricata, Carum copticum, Cassia occidentalis, Uncaria rhynchophylla, Viola odorata and Zingiber officinale (Nwokocha et al , 2012a;Gilani et al , 2005;Ajagbonna et al , 2001;Horie et al , 1992;Siddiqi et al , 2012;Ghayur and Gilani, 2005;Ojulari et al;Elkhishin and Awwad, 2009). Thymol, an active constituent from C. copticum, was identified and a dose dependent fall in blood pressure and heart rate was observed after its administration (Aftab and Usmanghani, 1995).…”

Section: Traditional Medicinal Plants With Reported Mode Of Action Wmentioning

confidence: 94%

Anti-hypertensive medicinal plants and their mode of action

Rawat

Singh

Kumar

2016

Journal of Herbal Medicine

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Effects of hirsutine, an antihypertensive indole alkaloid from Uncaria rhynchophylla, on intracellular calcium in rat thoracic aorta

Cited by 70 publications

References 23 publications

Corynoxeine Isolated from the Hook of Uncaria rhynchophylla Inhibits Rat Aortic Vascular Smooth Muscle Cell Proliferation through the Blocking of Extracellular Signal Regulated Kinase 1/2 Phosphorylation

Corynoxeine Isolated from the Hook of Uncaria rhynchophylla Inhibits Rat Aortic Vascular Smooth Muscle Cell Proliferation through the Blocking of Extracellular Signal Regulated Kinase 1/2 Phosphorylation

New vasorelaxant indole alkaloids, villocarines A–D from Uncaria villosa

Anti-hypertensive medicinal plants and their mode of action

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