Effects of high-density lipoproteins on storage at 4 C of fowl spermatozoa

Blesbois, E.; Hermier, Dominique

doi:10.1530/jrf.0.0900473

Cited by 13 publications

(12 citation statements)

References 15 publications

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“…In the absence of accessory glands in the deferent ducts of birds, seminal plasma contains molecules secreted (or excreted) at various locations of the vas deferens. They include lipoproteins (Blesbois and Hermier, 1990), a variety of proteins (the main protein being a serum-like albumin: Blesbois and Caffin, 1992), several sugars (including fructose but not glucose) and many other biological co-factors (Etches, 1996). Overall, the action of seminal plasma is complex during in vitro storage.…”

Section: Storage Of Avian Sperm In a Liquid Statementioning

confidence: 99%

“…In contrast, the anti-oxidant enzymes and a fraction of high molecular weight also present in the seminal plasma are beneficial to the in vitro storage of avian sperm (Blesbois and de Reviers, 1992;Surai et al, 1998). However, the presence of various toxic components, either naturally present in seminal plasma or added to it at the time of ejaculation or as the result of sperm catabolism, results overall in improved conditions of in vitro storage if the initial seminal plasma is discarded (Blesbois, 1990;Blesbois and Hermier, 1990;Douard et al, 2005) or replaced by dialysed seminal plasma (Blesbois and De Reviers, 1992;Iaffaldano and Meluzzi, 2003).…”

Section: Storage Of Avian Sperm In a Liquid Statementioning

confidence: 99%

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Specific features of in vivo and in vitro sperm storage in birds

Blesbois

Brillard²

2007

Animal

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This review focuses on some of the main features of sperm selection and storage in birds mainly on the basis of studies performed in poultry species, with emphasis on the initial selection of sperm at the female vagina level prior to migration towards the sperm storage tubules. Sperm originating from low-quality males or subjected to inappropriate in vitro storage conditions are rapidly discarded, resulting in impaired fertility in corresponding flocks. In the absence of accessible and appropriate technology for matching the 'storing' potential of sperm in the oviduct, conditions for prolonged sperm storage under a liquid (through the use of semen extenders) or a solid state (cryopreservation) have received only limited attention, despite their potential interest to facilitate male and female management in poultry flocks. Despite this, technology for shortterm liquid storage is currently used in turkeys, guinea fowl and muscovy ducks and also in progress in chickens. In addition, technology for cryopreservation of avian semen has become available for some species (chicken, goose) to facilitate the management of genetic resources, including the preservation of rare and economically important breeds.

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Section: Storage Of Avian Sperm In a Liquid Statementioning

confidence: 99%

Section: Storage Of Avian Sperm In a Liquid Statementioning

confidence: 99%

Specific features of in vivo and in vitro sperm storage in birds

Blesbois

Brillard²

2007

Animal

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“…Semen is composed of a complex mixture of organic and inorganic components built by secretions of the male reproductive organs, which the ejaculated spermatozoa bathe in (Mann et al, 1982;Blesbois and Hermier, 1990). In mammals, seminal plasma contains a huge bulk of proteins and peptides (25-60 g/L) per ejaculate, mostly derived from the accessory sex glands (Batruch et al, 2011;Rodríguez-Martínez et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Selection for higher fertility reflects in the seminal fluid proteome of modern domestic chicken

Atikuzzaman

Sanz

Plá

et al. 2017

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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The high egg-laying capacity of the modern domestic chicken (i.e. White Leghorn, WL) has arisen from the low egg-laying ancestor Red Junglefowl (RJF) via continuous trait selection and breeding. To investigate whether this long-term selection impacted the seminal fluid (SF)-proteome, 2DE electrophoresis-based proteomic analyses and immunoassays were conducted to map SF-proteins/cytokines in RJF, WL and a 9th generation Advanced Intercross Line (AIL) of RJF/WL-L13, including individual SF (n=4, from each RJF, WL and AIL groups) and pools of the SF from 15 males of each group, analyzed by 2DE to determine their degree of intra-group (AIL, WL, and RJF) variability using Principal Component Analysis (PCA); respectively an inter-breed comparative analysis of intergroup fold change of specific SF protein spots intensity between breeds. The PCA clearly highlighted a clear intra-group similarity among individual roosters as well as a clear inter-group variability (e.g. between RJF, WL and AIL) validating the use of pools to minimize confounding individual variation. Protein expression varied considerably for processes related to sperm motility, nutrition, transport and survival in the female, including signaling towards immunomodulation. The major conserved SF-proteins were serum albumin and ovotransferrin. Aspartate aminotransferase, annexin A5, arginosuccinate synthase, glutathione S-transferase 2 and l-lactate dehydrogenase-A were RJF-specific. Glyceraldehyde-3-phosphate dehydrogenase appeared specific to the WL-SF while angiotensin-converting enzyme, γ-enolase, coagulation factor IX, fibrinogen α-chain, hemoglobin subunit α-D, lysozyme C, phosphoglycerate kinase, Src-substrate protein p85, tubulins and thioredoxin were AIL-specific. The RJF-SF contained fewer immune system process proteins and lower amounts of the anti-inflammatory/immunomodulatory TGF-β2 compared to WL and AIL, which had low levels- or lacked pro-inflammatory CXCL10 compared to RJF. The seminal fluid proteome differs between ancestor and modern chicken, with a clear enrichment of proteins and peptides related to immune-modulation for sperm survival in the female and fertility.

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“…This biological medium presents a neutral (or slightly basic) pH and its osmolarity is sustained by a high content of amino acids such as glutamate and different proteins, the most abundant being a serum-like albumin (Blesbois and Caffin, 1992). It contains high levels of cholesterol and phospholipids originating from numerous lipidic and lipoproteic (high density lipoproteins, very high density lipoproteins) vesicules (Blesbois and Hermier, 1990;Douard et al, 2000). Seminal plasma stimulates sperm motility and is useful at the time of ejaculation.…”

Section: The Male Gamete At Ejaculationmentioning

confidence: 99%

Biological Features of the Avian Male Gamete and their Application to Biotechnology of Conservation

Blesbois¹

2012

J. Poult. Sci.

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Male gamete biology shows specific features in birds that contribute to the reproductive strategy of flying animals adapted to highly variable environmental conditions. In this paper, we review the main characteristics of bird sperm, their selection and storage in the highly specialized female oviduct, specific features of polyspermic fertilization, and the consequences of these biological adaptations on semen biotechnology. Among other features, the storage of sperm in specific oviducal glands is a key factor to increase reproductive "freedom" but is also a critical point for the success of semen in vitro storage.

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Effects of high-density lipoproteins on storage at 4 C of fowl spermatozoa

Cited by 13 publications

References 15 publications

Specific features of in vivo and in vitro sperm storage in birds

Specific features of in vivo and in vitro sperm storage in birds

Selection for higher fertility reflects in the seminal fluid proteome of modern domestic chicken

Biological Features of the Avian Male Gamete and their Application to Biotechnology of Conservation

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