Effects of hemodialysis on plasma oxylipins

Abstract

Chronic kidney disease (CKD) is an important risk factor for cardiovascular and all-cause mortality. The 5-year survival rate among end-stage renal disease (ESRD) hemodialysis patients is nearly 50% (McGill, 2019) and most of these deaths are related to cardiovascular disease (CVD), making ESRD a catastrophic risk factor (Luft, 2000). Conceivably, the hemodialysis treatment per se has a counterproductive effect on cardiovascular risk. Oxylipins are a superclass of lipid mediators with potent biological activities. They are derived from the oxidation of polyunsaturated fatty acids (PUFA). In addition to the wellknown eicosanoids derived from arachidonic acid (C20:4 n-6, AA), recent developments in lipidomics methodologies have raised awareness of, and interest in, the many hitherto unknown oxylipins. These products include octadecanoids derived from

Hemodialysis and biotransformation of erythrocyte epoxy fatty acids in peripheral tissue

“…Moreover, similar results were found for free erythrocyte epoxy-metabolites (Table 3B). Together, the findings indicate that CYP epoxy-metabolites are unequally hydrolyzed by sEH in arterial and venous blood in vivo [14,18]. However, there is no arteriovenous difference in the erythro-epoxides before hemodialysis.…”

Hemodialysis and erythrocyte epoxy fatty acids

“…Furthermore, hemodialysis treatment is insufficient to change the total concentrations of these and other LOX/CYP metabolites in RBCs of ESRD patients. Since the four subclasses of CYP epoxy metabolites increase in plasma after the dialysis treatment (Gollasch et al., 2020), we suggest that total CYP metabolites in RBCs are relatively invulnerable in CKD and hemodialysis (possibly due to slow exchange). Of note, ESRD is associated with increased levels of several free CYP epoxides and LOX/CYP ω/(ω‐1)‐hydroxylase metabolites in RBCs.…”

“…All blood samples were obtained by 4°C precooled EDTA vacuum extraction tube systems. Cells were separated from plasma by centrifugation for 10 min at 1,000–2,000 g using a refrigerated centrifuge RBCs were separated from EDTA blood by centrifugation as previously described (Gollasch, et al., 2020). RBC lipidomics was performed using LC–MS/MS tandem mass spectrometry as described in (Fischer et al., 2014; Gollasch et al., 2019; Gollasch et al., 2019).…”

Norepinephrine Inhibits Lipopolysaccharide-Stimulated TNF-α but Not Oxylipin Induction in n-3/n-6 PUFA-Enriched Cultures of Circumventricular Organs

“…A broad overview of the relationship between enzymes, oxylipins, and their substrates is shown in Figure 5 , contextualizing our results. However, such an approach can only capture a simplified high-level scheme of a continuously changing body of evidence for underlying pathways [ 6 , 99 , 100 ].…”

“…Docosahexaenoic-derived NPD1 (neuroprotectin D1) was observed to be increased by LPS stimulation. Metabolic pathways are visualized according to [ 6 , 99 , 100 ].…”