Effects of Heat Acclimation on Changes in Oxidative Stress and Inflammation Caused by Endurance Capacity Test in the Heat

Abstract

Background. The aim was to determine the effect of heat acclimation (HA) on oxidative stress (OxS) and inflammation in resting conditions and on the response pattern of these parameters to exhausting endurance exercise. Methods. Parameters of OxS and inflammation were measured in non-heat-acclimated status (NHAS) and after a 10-day HA program (i.e., in heat-acclimated status; HAS) both at baseline and after an endurance capacity (EC) test in the heat. Results. As a result of HA, EC increased from 88.62 ± 27.51 to 161.95 ± 47.80 minutes (P < 0.001). HA increased OxS level: total peroxide concentration rose from 219.38 ± 105.18 to 272.57 ± 133.39 μmol/L (P < 0.05) and oxidative stress index (OSI) from 14.97 ± 8.24 to 20.46 ± 11.13% (P < 0.05). In NHAS, the EC test increased OxS level: total peroxide concentration rose from 219.38 ± 105.18 to 278.51 ± 125.76 μmol/L (P < 0.001) and OSI from 14.97 ± 8.24 to 19.31 ± 9.37% (P < 0.01). However, in HAS, the EC test reduced OSI from 20.46 ± 11.13 to 16.83 ± 8.89% (P < 0.05). The value of log high-sensitive C-reactive protein increased from −0.32 ± 0.32 to −0.12 ± 0.34 mg/L (P < 0.05) in NHAS and from −0.31 ± 0.47 to 0.28 ± 0.46 mg/L (P < 0.001) in HAS. Conclusion. HA increases OxS level. However, beneficial adaptive effects of HA on acute exhaustive exercise-induced changes in OxS and inflammation parameters occur in a hot environment.

Integrated Metabolomics Study of the Milk of Heat-stressed Lactating Dairy Cows

“…Increased c-reactive protein, TNF-α, IL-2, IL-10, IL-15, and decreased IL-12 in both the plasma and milk of HS group are regarded as a response to HS-induced inflammation 29 33 34 , confirming the impairment of the immune system in the HS group. The plasma levels of cytochrome c and c-reactive protein were significantly correlated with their respective concentrations in milk ( Supplementary Table S10 ), suggesting that HS induced an inflammatory response of the whole body of dairy cows 33 . During a mammary immune response, the blood-milk barrier is compromised 35 .…”

Protective Effects of Inorganic and Organic Selenium on Heat Stress in Bovine Mammary Epithelial Cells

“…Meanwhile, there are over 58% of dairy cows living in the area of torrid and subtropical zones where the temperature humidity index (THI) often reaches over 68 which causes heat stress in dairy cows [2]. Heat stress induces oxidative stress and inflammation, increases the risk of health problems, and reduces milk production [3]. Thus, it is of vital importance to explore effective methods to mitigate the suffering of dairy cows and reduce economic losses in heat stress.…”

Antipsychotic Treatment Reduces Indices of Oxidative Stress in First‐Episode Psychosis Patients

“…Blood (5 mL) was sampled in anticoagulant-free tubes and kept for 1 hour at 4°C (for platelet activation) before serum was isolated (centrifugation at 2000 × 15 minutes at 4°C). Serum was kept at −70°C before testing and all following procedures were performed similarly to our previous studies [ 17 ]. The clinical levels of triglycerides, total cholesterol, low-density lipoproteins (LDL cholesterol), high-density lipoproteins (HDL cholesterol), C-reactive protein (CRP), and glycated hemoglobin (HbA1c) were determined by standard laboratory methods using certified assays in the local clinical laboratory.…”

“…Within- and between-batch precision data obtained by TAC method were 2.5% and 2.9%, respectively. Percent ratio of the TPX to the TAC is used as OxS index (OSI), an indicator of the degree of OxS [ 17 ]. Thus, OSI was calculated as follows: OSI = [(TPX, μ mol/L):((TAC, μ mol Trolox/L) × 100)].…”