Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Cas9 in Rice

Chen, Yuyu; Zhu, Anning; Xue, Peng; Wen, Xiaopeng; Cao, Yang; Wang, Beifang; Zhang, Yue; Shah, Liaqat Ali; Cheng, Shifeng; Zhang, Yingxin; Zhang, Yingxin

doi:10.1016/j.rsci.2019.12.010

Cited by 43 publications

(14 citation statements)

References 38 publications

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“…Among them, few significant QTLs, such as OsGS3 (which encodes a Gγ-subunit of heterotrimeric G-protein) and GL3.1 (which encodes a putative serine/threonine phosphatase OsPPKL1 with a kelch-like repeat domain), negatively regulate grain size and thus impact grain yield. CRISPR-based editing of OsGS3 and OsGL3.1 improved grain size, which ultimately increased the 1000-grain weight and overall yield per plant in rice (Figure e) . Multiplex gene editing of three genes ( GS3 , GW2 , and Gn1a ) also improved grain yield in edited plants compared with wild type .…”

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 91%

“…CRISPR-based editing of OsGS3 and OsGL3.1 improved grain size, which ultimately increased the 1000-grain weight and overall yield per plant in rice (Figure 3e). 51 Multiplex gene editing of three genes (GS3, GW2, and Gn1a) also improved grain yield in edited plants compared with wild type. 52 In another study, OsPIN5b (which controls panicle length), GS3, and OsMYB30 (which regulates drought tolerance in rice), were knocked out simultaneously with CRISPR-Cas9 and generated high-yielding droughtresistant rice lines.…”

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 98%

“…Currently, 19 QTLs that determine grain size have been cloned and characterized in rice. 51 Among them, few significant QTLs, such as OsGS3 (which encodes a Gγ-subunit of heterotrimeric G-protein) and GL3.1 (which encodes a putative serine/threonine phosphatase OsPPKL1 with a kelch-like repeat domain), negatively regulate grain size and thus impact grain yield. CRISPR-based editing of OsGS3 and OsGL3.1 improved grain size, which ultimately increased the 1000-grain weight and overall yield per plant in rice (Figure 3e).…”

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 99%

“…Grain yield is controlled by quantitative trait loci (QTLs) that affect 1000-grain weight (including grain size), number of grains per panicle, number of florets per panicle, and number of panicles per plant. Currently, 19 QTLs that determine grain size have been cloned and characterized in rice . Among them, few significant QTLs, such as OsGS3 (which encodes a Gγ-subunit of heterotrimeric G-protein) and GL3.1 (which encodes a putative serine/threonine phosphatase OsPPKL1 with a kelch-like repeat domain), negatively regulate grain size and thus impact grain yield.…”

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 99%

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CRISPR-Based Crop Improvements: A Way Forward to Achieve Zero Hunger

Ahmad

Tang

Shahzad

et al. 2021

J. Agric. Food Chem.

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Zero hunger is one of the sustainable development goals set by the United Nations in 2015 to achieve global food security by 2030. The current harvest of crops is insufficient; feeding the world’s population and meeting the goal of zero hunger by 2030 will require larger and more consistent crop production. Clustered regularly interspaced short palindromic repeats-associated protein (CRISPR-Cas) technology is widely used for the plant genome editing. In this review, we consider this technology as a potential tool for achieving zero hunger. We provide a comprehensive overview of CRISPR-Cas technology and its most important applications for food crops’ improvement. We also conferred current and potential technological breakthroughs that will help in breeding future crops to end global hunger. The regulatory aspects of deploying this technology in commercial sectors, bioethics, and the production of transgene-free plants are also discussed. We hope that the CRISPR-Cas system will accelerate the breeding of improved crop cultivars compared with conventional breeding and pave the way toward the zero hunger goal.

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Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 91%

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 98%

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 99%

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 99%

See 2 more Smart Citations

CRISPR-Based Crop Improvements: A Way Forward to Achieve Zero Hunger

Ahmad

Tang

Shahzad

et al. 2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…GS3 ( GRAIN SIZE 3 ), the first QTL identified in regulating grain length, has been successfully knocked out in five japonica rice varieties. The grain length of the T1 lines in all different genetic backgrounds has been increased compared to wild type [ 25 , 26 ]. Grain shape affects not only quality but also grain weight (GW), for example, rice GW has been increased by disruption of multiple grain weight negative regulators, GW2 , GW5 , and GW6 [ 27 ].…”

Section: Crispr/cas9-mediated Molecular Breeding Accelerates Crop Qualitymentioning

confidence: 99%

Application of CRISPR/Cas9 in Crop Quality Improvement

Liu

Yang

Zhang

et al. 2021

IJMS

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The various crop species are major agricultural products and play an indispensable role in sustaining human life. Over a long period, breeders strove to increase crop yield and improve quality through traditional breeding strategies. Today, many breeders have achieved remarkable results using modern molecular technologies. Recently, a new gene-editing system, named the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology, has also succeeded in improving crop quality. It has become the most popular tool for crop improvement due to its versatility. It has accelerated crop breeding progress by virtue of its precision in specific gene editing. This review summarizes the current application of CRISPR/Cas9 technology in crop quality improvement. It includes the modulation in appearance, palatability, nutritional components and other preferred traits of various crops. In addition, the challenge in its future application is also discussed.

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Plant Genome Editing: Advances and Prospects of Market‐Ready Food Crops

Heynes¹,

Wang²,

Lu³

2022

Annual Plant Reviews Online

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Genome‐editing biotechnologies, such as clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐associated proteins (Cas), are disrupting both the research and commercial genomics industries. Genome editing has become a major tool within both the Fourth Industrial Revolution and Green Revolution 2.0, making claim to revolutionise healthcare, environmental conservation, agriculture, and food security. With the technology rapidly advancing the fields of plant genomics and breeding, an increasing number of gene‐edited food crops are now launching to both industry and consumer markets. New market‐ready gene‐edited food crops and products include a healthier soya bean oil and the first consumer‐harvested CRISPR/Cas crop, the Sicilian Rouge High GABA tomato. However, as gene‐edited crops begin to arrive on the market, debates previously seen with GMOs surrounding social acceptance, bioethics, and changing regulatory environments for agriculture and food safety are being renewed. In this article, we provide an overview of plant genome‐editing technologies, including CRISPR/Cas, transcription activator‐like effector nucleases (TALENs), and zinc finger nucleases (ZFNs); market‐ready gene‐edited food crops; and regulatory environments across the world.

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Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Cas9 in Rice

Cited by 43 publications

References 38 publications

CRISPR-Based Crop Improvements: A Way Forward to Achieve Zero Hunger

CRISPR-Based Crop Improvements: A Way Forward to Achieve Zero Hunger

Application of CRISPR/Cas9 in Crop Quality Improvement

Plant Genome Editing: Advances and Prospects of Market‐Ready Food Crops

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