Effects of Griseoviridin and Viridogrisein against Swine Dysentery in Experimental Infection by Using Mice and Pigs

Asano, Takashi; Adachi, Yoshikazu

doi:10.1292/jvms.68.555

Cited by 7 publications

(1 citation statement)

References 14 publications

(14 reference statements)

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“…Those promising results have emphasized the need to continue the research in this way. For example, griseoviridin, a broad spectrum antibiotic of group A consisting of a series of 23-membered macrolactones with 2,4-disubstituted oxazole, (E,E)-dienylamine and 1,3-dioxygenation, exhibits in vitro inhibitory activity towards various pathogenic bacteria and fungi, and it also inhibits bacterial protein synthesis and poly (U)-directed poly (phenylalanine) synthesis in yeast and in human tonsil cell free systems (Dvorak et al 2000;Asano and Adachi 2006). The stereo-chemical structure of griseoviridin was comprehensively investigated by NMR spectra (Jayaraman et al 1994;Liu and Lin 2009).…”

Section: Introductionmentioning

confidence: 99%

A new marine microorganism strain L0804: taxonomy and characterization of active compounds from its metabolite

Lin

Liu

2010

World J Microbiol Biotechnol

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A new streptomyces strain designated L0804, producing antimicrobial substances, was isolated from marine mud in Qiangang, Jiangsu province, China. On the basis of cellular morphology, physiological and chemotaxonomic characterization and phylogenetic similarity of 16S rDNA gene sequences, the strain L0804 was identified as Streptomyces roseogilvus var. marine. However, the phylogenetic analysis indicated that strain L0804 represents a distinct phyletic line suggesting a new genomic species. Two antimicrobial compounds were isolated and purified from the strain L0804 culture broth using various separation procedures. The two active compounds were elucidated by HPLC, UV-VIS, IR and NMR spectroscopy. One was a mixture of at least three compounds belonging to alkaloids homologue, the other was griseoviridin. The antimicrobial activity and cytotoxicity of the two active compounds were compared. The results showed that strain L0804 was a new potential marine microorganism producing griseoviridin. Simultaneously, strain L0804 can also produce valuable substance of alkaloids homologue.

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Section: Introductionmentioning

confidence: 99%

A new marine microorganism strain L0804: taxonomy and characterization of active compounds from its metabolite

Lin

Liu

2010

World J Microbiol Biotechnol

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Structural analysis of SgvP involved in carbon–sulfur bond formation during griseoviridin biosynthesis

Qin

Chen²,

Zhang

et al. 2017

FEBS Letters

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Identification of the Biosynthetic Gene Cluster and Regulatory Cascade for the Synergistic Antibacterial Antibiotics Griseoviridin and Viridogrisein in Streptomyces griseoviridis

et al. 2012

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Griseoviridin (GV) and viridogrisein (VG, also referred to as etamycin), produced by Streptomyces griseoviridis, are two chemically unrelated compounds belonging to the streptogramin family. Both of these natural products demonstrate broad-spectrum antibacterial activity and constitute excellent candidates for future drug development. To elucidate the biosynthetic machinery associated with production of these two unique antibiotics, the gene cluster responsible for both GV and VG production was identified within the Streptomyces griseoviridis genome and characterized, and its function in GV and VG biosynthesis was confirmed by inactivation of 30 genes and complementation experiments. This sgv gene cluster is localized to a 105 kb DNA region that consists of 36 open reading frames (ORFs), including four nonribosomal peptide synthetases (NRPSs) for VG biosynthesis and a set of hybrid polyketide synthases (PKS)-NRPSs with a discrete acyltransferase (AT), SgvQ, to assemble the GV backbone. The enzyme encoding genes for VG versus GV biosynthesis are separated into distinct "halves" of the cluster. A series of four genes: sgvA, sgvB, sgvC, and sgvK, were found downstream of the PKS-NRPS; these likely code for construction of a γ-butyrolactone (GBL)-like molecule. GBLs and the corresponding GBL receptor systems are the highest ranked regulators that are able to coordinate the two streptomyces antibiotic regulatory protein (SARP) family positive regulators SgvR2 and SgvR3; both are key biosynthetic activators. Models of GV, VG, and GBL biosynthesis were proposed by using functional gene assignments, determined on the basis of bioinformatics analysis and further supported by in vivo gene inactivation experiments. Overall, this work provides new insights into the biosyntheses of the GV and VG streptogramins that are potentially applicable to a host of combinatorial biosynthetic scenarios.

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Effects of Griseoviridin and Viridogrisein against Swine Dysentery in Experimental Infection by Using Mice and Pigs

Cited by 7 publications

References 14 publications

A new marine microorganism strain L0804: taxonomy and characterization of active compounds from its metabolite

A new marine microorganism strain L0804: taxonomy and characterization of active compounds from its metabolite

Structural analysis of SgvP involved in carbon–sulfur bond formation during griseoviridin biosynthesis

Identification of the Biosynthetic Gene Cluster and Regulatory Cascade for the Synergistic Antibacterial Antibiotics Griseoviridin and Viridogrisein in Streptomyces griseoviridis

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