Effects of green light-emitting diode irradiation on neural differentiation of human umbilical cord matrix-derived mesenchymal cells; Involvement of MAPK pathway

Seyyedin, Sajad; Shojaei, Mohammad Reza; Fallah, Hossein; Khosravi, Ahmad; Nematollahi-Mahani, Seyed Noureddin

doi:10.1016/j.bbrc.2022.11.028

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…Recently, light-emitting diode (LED) irradiation has been used to treat several clinical conditions, including wound healing, pain, bone regeneration and treatment of various diseases and infections [2]. We have reported that LED irradiation can result in various biological effects, such as mouse hepatocyte proliferation and colon cancer cell autophagy [3,4].…”

Section: Introductionmentioning

confidence: 99%

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Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Waki

Saito

Chen

et al. 2023

Preprint

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Background Light-emitting diode (LED) irradiation has been used in the differentiation of mesenchymal stem cells into a variety of cell types. This study investigated the effect of green LED (GLED) irradiation on the differentiation of adipocyte-derived mesenchymal cells into hepatocyte-like cells (HLCs) and the mechanism of its action. Methods HLCs in the hepatocyte maturation phase were irradiated with GLED (520 nm, 21 W/m2, 5 min/day for 10 days). The cells were then assessed for expression of hepatocyte maturity genes and opsin 3 (OPN3), hepatocyte function, viability, apoptosis, and levels of reactive oxygen species (ROS), intracellular adenosine triphosphate (ATP) and calcium ions (Ca2+). Results GLED irradiation increased Alpha-1 antitrypsin and Ornithine transcarbamylase gene expression, promoted Cytochrome P450 3A4 activity and urea synthesis, and elevated intracellular ROS, ATP and Ca2+ levels. OPN3 expression was significantly more upregulated in GLED-irradiated HLCs than in the non-irradiated HLCs. No significant difference in cell viability or apoptosis was observed between GLED-irradiated and non-irradiated HLCs. Conclusions GLED irradiation can promote hepatocyte maturation and functions through OPN3. GLED irradiation also stimulated mitochondrial function via Ca2+/ATP/ROS activation. GLED irradiation has potential to support cell-based transplantation in patients.

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Section: Introductionmentioning

confidence: 99%

“…The effect of LED irradiation at different wavelengths and energy densities also improves the regenerative capacity of various types of MSC [5,6]. Green LED (GLED) irradiation promotes the differentiation of MSCs into neurons and osteoblasts [2,7]. However, it is not known whether GLED can promote the hepatic differentiation of HLCs.…”

Section: Introductionmentioning

confidence: 99%

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Waki

Saito

Chen

et al. 2023

Preprint

View full text Add to dashboard Cite

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A comprehensive review on therapeutic potentials of photobiomodulation for neurodegenerative disorders

Ramakrishnan,

Joshi,

Fazil

et al. 2024

Life Sciences

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Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Waki,

Saito,

Chen

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Light-emitting diode (LED) irradiation has been used in the differentiation of mesenchymal stem cells into a variety of cell types. This study investigated the effect of green LED (GLED) irradiation on the differentiation of adipocyte-derived mesenchymal cells into hepatocyte-like cells (HLCs) and the mechanism of its action. HLCs in the hepatocyte maturation phase were irradiated with GLED (520 nm, 21 W/m2, 5 min/day for 10 days). The cells were then assessed for expression of hepatocyte maturity genes and opsin 3 (OPN3), hepatocyte function, viability, apoptosis, and levels of reactive oxygen species (ROS), intracellular adenosine triphosphate (ATP) and calcium ions (Ca2+). GLED irradiation increased Alpha-1 antitrypsin and Ornithine transcarbamylase gene expression, promoted Cytochrome P450 3A4 activity and urea synthesis, and elevated intracellular ROS, ATP and Ca2+ levels. OPN3 expression was significantly more upregulated in GLED-irradiated HLCs than in the non-irradiated HLCs. No significant difference in cell viability or apoptosis was observed between GLED-irradiated and non-irradiated HLCs. GLED irradiation can promote hepatocyte maturation and functions through OPN3. GLED irradiation also stimulated mitochondrial function via Ca2+/ATP/ROS activation. GLED irradiation has potential to support cell-based transplantation in patients.

show abstract

Effects of green light-emitting diode irradiation on neural differentiation of human umbilical cord matrix-derived mesenchymal cells; Involvement of MAPK pathway

Cited by 4 publications

References 38 publications

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

A comprehensive review on therapeutic potentials of photobiomodulation for neurodegenerative disorders

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

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