Effects of granulocyte colony-stimulating factor on bacterial translocation due to burn wound sepsis

Abstract

The presence of certain defects in both cellular and humoral immunity after thermal injury has been established. Likewise, the translocation of enteric bacteria to the mesenteric lymph nodes and to distant organs has also been observed following serious thermal injury. The effects of granulocyte colony-stimulating factor (G-CSF) on bacterial translocation, the small bowel mucosa, and cecal bacterial content were investigated in a rat model of burn wound sepsis in which albino Wistar rats were scalded over 30% of their bodies, after which the lesions were infected by 1 x 10(8) colony-forming units (cfu) Pseudomonas aeruginosa. The control group was treated with 5% dextrose solution subcutaneously starting 2 days preburn, while the treatment group received 100 microg/kg human G-CSF subcutaneously. On the 4th day post burn all animals were killed to examine the bowel and culture of the mesenteric lymph nodes (MLN), livers, and spleens. No significant differences were observed between the groups regarding the cecal bacterial content and small bowel; however, a difference was seen in the ratio of translocation in the MLN liver and spleen and quantitative MLN cultures. Based on these findings, G-CSF was thus found to be significantly effective in reducing bacterial translocation due to burn wound sepsis.

Effect of Different Enteral Nutrients on Bacterial Translocation in Experimental Obstructive Jaundice

“…Therefore, we suggest that the ability of immunonutrition to prevent BT is probably related to improvement of host immunity. Our finding supports many other studies which have shown that BT was prevented without improvement in intestinal mucosal barrier [38][39][40]. In a rat model of burn wound sepsis, Yalcin et al [38] reported that both the control and granulocyte colony-stimulating factor (g-csf) treated rats had disrupted mucosal barrier manifested with blunted villae in distal ileum.…”

“…Our finding supports many other studies which have shown that BT was prevented without improvement in intestinal mucosal barrier [38][39][40]. In a rat model of burn wound sepsis, Yalcin et al [38] reported that both the control and granulocyte colony-stimulating factor (g-csf) treated rats had disrupted mucosal barrier manifested with blunted villae in distal ileum. They demonstrated that although no improvement was seen in the mucosal barrier in g-csftreated rats bacterial translocation was significantly decreased.…”

Relevance and Perspectives of Experimental Wound Models in Wound Healing Research

“…Full thickness burn injury is induced in all rats according to the methods of Walker and Yalcin [17,18]. In this method, rats are anesthetized, and hair on the back of each rat is removed with an electric clipper.…”

Infected animal models for tissue engineering

“…In this model, approximately 30% of the total body surface area of a mouse was burned by exposure to a flame for 10 s followed by inoculation with Pseudomonas aeruginosa . This procedure was modified by Rumbaugh et al and others to create a burn wound by use of scalding water (90°C) rather than open flame [52] and also applied to rats [53]. The dorsal cutaneous burn wound models have been used to explore pathogen quorum sensing [52], bacterial migration and development of sepsis from infected burn wounds [29], and different therapies [54–56].…”