Effects of gibberellic acid on hairy root cultures of Artemisia annua: Growth and artemisinin production

Smith, T. C.; Weathers, Pamela J.; Cheetham, R. D.

doi:10.1007/s11627-997-0044-4

Cited by 68 publications

(38 citation statements)

References 23 publications

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“…A combination of benzyladenine and kinetin increased the yields of artemisinin in cultured shoots by 3.6 and 2.6 fold (Whipkey et al 1992). Gibberellic acid, a plant hormone that can induce blooming, has been reported to improve growth and artemisinin biosynthesis in shoot cultures, root cultures and plantlets of A. annua (Fulzele et al 1995;Smith et al 1997;Weathers et al 2005;Zhang et al 2005). According to Liersch et al (1986), chlormequat was also able to increase the artemisinin content by 30%.…”

Section: Resultsmentioning

confidence: 99%

Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Jing

Zhang

et al. 2009

Biologia

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Artemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L., is the most effective antimalarial drug. In an effort to increase the artemisinin production, abscisic acid (ABA) with different concentrations (1, 10 and 100 µM) was tested by treating A. annua plants. As a result, the artemisinin content in ABA-treated plants was significantly increased. Especially, artemisinin content in plants treated by 10 µM ABA was 65% higher than that in the control plants, up to an average of 1.84% dry weight. Gene expression analysis showed that in both the ABA-treated plants and cell suspension cultures, HMGR, FPS, CYP71AV1 and CPR, the important genes in the artemisinin biosynthetic pathway, were significantly induced. While only a slight increase of ADS expression was observed in ABA-treated plants, no expression of ADS was detected in cell suspension cultures. This study suggests that there is probably a crosstalk between the ABA signaling pathway and artemisinin biosynthetic pathway and that CYP71AV1, which was induced most significantly, may play a key regulatory role in the artemisinin biosynthetic pathway.

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Section: Resultsmentioning

confidence: 99%

Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Jing

Zhang

et al. 2009

Biologia

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“…Cultures of the YUT16 hairy root clone of Artemisia annua L. (Weathers et al, 1994) were grown at 100 RPM (25.4 mm orbit diameter; Lab-Line Instruments, Melrose Park, IL) in 125-mL shake flasks containing 50 mL of Gamborg's B5 medium (Gamborg et al, 1968) with half strength CaCl 2 и 2H 2 O and 30 g/L sucrose, subcultured every 2 weeks (Wobbe et al, 1998;Smith et al, 1997). All experiments were performed under continuous cool white fluorescent light (4 ± 0.5 E/m 2 /s) and at 23 ± 2°C.…”

Section: Hairy Rootsmentioning

confidence: 99%

Growth of Artemisia annua hairy roots in liquid‐ and gas‐phase reactors

Kim

Weathers

Wyslouzil

2002

Biotech & Bioengineering

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Artemisia annua hairy roots were grown in liquid-phase bubble column and gas-phase nutrient mist reactors. In most cases the bubble column reactor accumulated more biomass than the mist reactor; the highest final biomass concentrations observed were 15.3 g DW/L in the bubble column reactor and 14.4 g DW/L in the mist reactor. Further analysis showed that the average specific growth rate in the mist reactors was essentially constant and independent of the biomass concentration at the beginning of the mist mode. In contrast, at low packing densities the average growth rate in the bubble column reactors was higher than in the mist reactors, decreasing to comparable rates at high packing densities. Finally, an aerosol deposition model was used to compare the volume of medium captured by the root bed in the mist reactor to the volume of medium required to maintain a specified growth rate. The results suggest that under the current operating conditions, lower growth rates in the mist reactor may be due to insufficient nutrient availability.

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“…Extraction of artemisinin was done according to a method (Smith et al 1997). The separation of sodium derivative of artemisinin was done using Agilent 1200 series HPLC (High-performance liquid chromatography) system, by a reversed-phase C18 analytical column using the mobile phase as 0.01 M sodium phosphate buffer and methanol (in 1:1 ratio).…”

Section: Methodsmentioning

confidence: 99%

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

Patra

Srivastava

2015

Plant Cell Rep

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Alternative biotechnological protocol for large-scale artemisinin production was established. It featured enhanced growth and artemisinin production by cultivation of hairy roots in nutrient mist bioreactor (NMB) coupled with novel cultivation strategies. Artemisinin is used for the treatment of cerebral malaria. Presently, its main source is from seasonal plant Artemisia annua. This study featured investigation of growth and artemisinin production by A. annua hairy roots (induced by Agrobacterium rhizogenes-mediated genetic transformation of explants) in three bioreactor configurations-bubble column reactor, NMB and modified NMB particularly to establish their suitability for commercial production. It was observed that cultivation of hairy roots in a non-stirred bubble column reactor exhibited a biomass accumulation of 5.68 g/l only while batch cultivation in a custom-made NMB exhibited a higher biomass concentration of 8.52 g/l but relatively lower artemisinin accumulation of 0.22 mg/g was observed in this reactor. A mixture of submerged liquid-phase growth (for 5 days) followed by gas-phase cultivation in nutrient mist reactor operation strategy (for next 15 days) was adopted for hairy root cultivation in this investigation. Reasonably, high (23.02 g/l) final dry weight along with the artemisinin accumulation (1.12 mg/g, equivalent to 25.78 mg/l artemisinin) was obtained in this bioreactor, which is the highest reported artemisinin yield in the gas-phase NMB cultivation.

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Effects of gibberellic acid on hairy root cultures of Artemisia annua: Growth and artemisinin production

Cited by 68 publications

References 23 publications

Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Growth of Artemisia annua hairy roots in liquid‐ and gas‐phase reactors

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

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