Effects of GH and IGF-I on the in vitro maturation of mouse oocytes

Kiapekou, Erasmia; Loutradis, Dimitris; Drakakis, Peter; Zapanti, Evangelia; Mastorakos, George; Antsaklis, A.

doi:10.14310/horm.2002.11153

Cited by 38 publications

(27 citation statements)

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“…Significant differences between cumulus expansion are indicated by different letters (P<0.05) knowledge, the localization and distribution of GHRs using immunocytochemistry in the rhesus macaque COC has not been reported. GHRs located on the oocyte and within the ooplasm in the rhesus macaque support the view that GH may have a direct effect on the oocyte as shown in the mouse [37]. The localization of GHRs on cumulus cell surfaces in immature macaque COCs also indicate that GH action on the oocyte may be mediated through cumulus cells similar to what is observed in the rat and bovine [7,8,38].…”

Section: Discussionsupporting

confidence: 72%

Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Prada

VandeVoort

2008

J Assist Reprod Genet

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Section: Discussionsupporting

confidence: 72%

Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Prada

VandeVoort

2008

J Assist Reprod Genet

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“…CD9 is a protein required for sperm binding to the oocyte (65,66). The effects of IGF1 on IVM have been difficult to interpret because the media used have often been chemically undefined (e.g., containing follicular fluid or FBS) (67)(68)(69)(70). We speculate that the beneficial effects we observed by using a chemically defined medium (Fig.…”

Section: Discussionmentioning

confidence: 94%

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Yuan

Spate

Redel

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

110

117

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Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to the blastocyst stage, and give rise to live young than their in vivo-produced counterparts, particularly if they are derived from immature females. Here we show that a chemically defined maturation medium supplemented with three cytokines (FGF2, LIF, and IGF1) in combination, so-called "FLI medium," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovaries and provides a twofold increase in the efficiency of blastocyst production after in vitro fertilization. Transfer of such blastocysts to recipient females doubles mean litter size to about nine piglets per litter. Maturation of oocytes in FLI medium, therefore, effectively provides a fourfold increase in piglets born per oocyte collected. As they progress in culture, the FLI-matured cumulus-oocyte complexes display distinctly different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte within. These events likely underpin the improvement in oocyte quality achieved by using the FLI medium.cumulus cell | embryo development | genetic modification | in vitro fertilization | MAPK signaling

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“…Co-treatment with GH improves the Gn effects on granulose cells. Regarding the use of GH, a study showed that mouse oocyte maturation was significantly affected by treating with GH and IGF-1, respectively or collectively [26] . A recent meta-analysis about different therapeutic protocols for ovarian stimulation of POR found that GH addition could improve clinical pregnancy rate and live birth rate, however the total numbers in the meta-analysis were small (251 patients) to draw any definitive conclusions.…”

Section: Discussionmentioning

confidence: 99%

The influence of different growth hormone addition protocols to poor ovarian responders on clinical outcomes in controlled ovary stimulation cycles

Wang

et al. 2017

Medicine

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Background:Growth hormone (GH) is used as an adjuvant therapy in in vitro fertilization and embryo transfer (IVF-ET) for poor ovarian responders, but findings for its effects on outcomes of IVF have been conflicting. The aim of the study was to compare IVF-ET outcomes among women with poor ovarian responders, and find which subgroup can benefit from the GH addition.Methods:We searched the databases, using the terms “growth hormone,” “GH,” “IVF,” “in vitro fertilization.” Randomized controlled trials (RCT) were included if they assessed pregnancy rate, live birth rate, collected oocytes, fertilization rate, and implantation rate. Extracted the data from the corresponding articles, Mantel–Haenszel random-effects model, or fixed-effects model was used. Eleven studies were included.Results:Clinical pregnancy rate (RR 1.65, 95% CI 1.23–2.22), live birth rate (RR1.73, 1.25–2.40), collected oocytes number (SMD 1.09, 95% CI 0.54–1.64), MII oocytes number (SMD 1.48, 0.84–2.13), and E2 on human chorionic gonadotropin (HCG) day (SMD 1.03, 0.18–1.89) were significantly increased in the GH group. The cancelled cycles rate (RR 0.65, 0.45–0.94) and the dose of gonadotropin (Gn) (SMD –0.83, –1.47, –0.19) were significantly lower in patients who received GH. Subgroup analysis indicated that the GH addition with Gn significantly increased the clinical pregnancy rate (RR 1.76, 1.25–2.48) and the live birth rate (RR 1.91, 1.29–2.83).Conclusion:The GH addition can significantly improve the clinical pregnancy rate and live birth rate. Furthermore, the GH addition time and collocation of medications may affect the pregnancy outcome.

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Effects of GH and IGF-I on the in vitro maturation of mouse oocytes

Cited by 38 publications

References 0 publications

Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

The influence of different growth hormone addition protocols to poor ovarian responders on clinical outcomes in controlled ovary stimulation cycles

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