Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Prada, Jenna K. Nyholt de; VandeVoort, Catherine A.

doi:10.1007/s10815-008-9208-3

Cited by 37 publications

(42 citation statements)

References 66 publications

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“…Previous studies using the rhesus model have noted that oocyte quality is improved with in vivo exposure to the maturing follicle [10,12,27,29]. This study is consistent with previous studies in which changes in gene expression within all follicular cell types were reported during the last 24 h of VVM with hCG [27,29], and with studies showing changes in oocyte gene expression related to cell-cell communication [27].…”

Section: Discussionsupporting

confidence: 91%

“…Adult female rhesus macaques (Macaca mulatta) were housed at the California National Primate Research Center, as previously described [10].…”

Section: Animal Husbandrymentioning

confidence: 99%

“…COCs were removed at 28-30 h following hCG by ultrasound-guided aspiration [11,12]. Oocytes were retrieved from aspirates as previously described [10]. Granulosa cells were obtained from the aspirates and immediately placed in buffer and stored frozen for later processing for gene expression.…”

Section: Animal Husbandrymentioning

confidence: 99%

“…Blastocyst-stage embryos were fixed and stained for differential cell counting with POU5F1 (also known as OCT3/4), as previously described [10]. Optical sectioning was performed with a Delta Vision microscope using a 203 Olympus UApo 340/0.70 water immersion objective (Olympus Optical Co., Ltd., Tokyo, Japan).…”

Section: Ivf and Embryo Developmentmentioning

confidence: 99%

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Differential Effects of Follistatin on Nonhuman Primate Oocyte Maturation and Pre-Implantation Embryo Development In Vitro1

VandeVoort

Mtango

Lee

et al. 2009

Biology of Reproduction

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There is a vital need to identify factors that enhance human and nonhuman primate in vitro embryo culture and outcome, and to identify the factors that facilitate that objective. Granulosa and cumulus cells were obtained from rhesus monkeys that had either been FSH-primed (in vitro maturation [IVM]) or FSH and hCG-primed (in vivo maturation [VVM]) and compared for the expression of mRNAs encoding follistatin (FST), inhibin, and activin receptors. The FST mRNA displayed marginally decreased expression (P ¼ 0.05) in association with IVM in the granulosa cells. The ACVR1B mRNA was more highly expressed in cumulus cells with IVM compared with VVM. Cumulus-oocyte complexes from FSH-primed monkeys exposed to exogenous FST during the 24-h IVM period exhibited no differences in the percentage of oocytes maturing to the metaphase II stage of meiosis compared to controls. However, embryos from these oocytes had significantly decreased development to the blastocyst stage. The effect of FST on early embryo culture was determined by exposing fertilized VVM oocytes to exogenous FST from 12 to 60 h postinsemination. FST significantly improved time to first cleavage and embryo development to the blastocyst stage compared with controls. The differential effects of exogenous FST on embryo development, when administered before and after oocyte maturation, may depend on the endogenous concentration in cumulus cells and oocytes. These results reveal evolutionary conservation of a positive effect of FST on embryogenesis that may be broadly applicable to enhance in vitro embryogenesis, with potential application to human clinical outcome and livestock and conservation biology.

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Section: Discussionsupporting

confidence: 91%

“…Adult female rhesus macaques (Macaca mulatta) were housed at the California National Primate Research Center, as previously described [10].…”

Section: Animal Husbandrymentioning

confidence: 99%

Section: Animal Husbandrymentioning

confidence: 99%

Section: Ivf and Embryo Developmentmentioning

confidence: 99%

See 2 more Smart Citations

Differential Effects of Follistatin on Nonhuman Primate Oocyte Maturation and Pre-Implantation Embryo Development In Vitro1

VandeVoort

Mtango

Lee

et al. 2009

Biology of Reproduction

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“…Adult female rhesus macaques (Macaca mulatta) were housed at the California National Primate Research Center (CNPRC) as described previously (47). Only females with a history of normal menstrual cycles were selected for this study.…”

Section: Methodsmentioning

confidence: 99%

Role for cumulus cell-produced EGF-like ligands during primate oocyte maturation in vitro

Prada¹,

Lee²,

Latham³

et al. 2009

American Journal of Physiology-Endocrinology and Metabolism

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The developmental competence of in vitro-matured (IVM) rhesus macaque cumulus oocyte complexes (COCs) is deficient compared with in vivo-matured (IVM) oocytes. To improve oocyte quality and subsequent embryo development following IVM, culture conditions must be optimized. A series of experiments was undertaken to determine the role of epidermal growth factor (EGF) during IVM of rhesus macaque COCs. The addition of Tyrphostin AG-1478 (a selective inhibitor of the EGF receptor EGFR) to the IVM medium yielded fewer oocytes maturing to metaphase II of meiosis II (MII), decreased cumulus expansion, and a lower percentage of embryos that developed to the blastocyst stage compared with untreated IVM controls, indicating that EGFR activation is important for IVM maturation in the rhesus macaque. However, the addition of recombinant human EGF (r-hEGF) to the IVM medium did not enhance outcome. The expression of mRNAs encoding the EGF-like factors amphiregulin, epiregulin, and betacellulin in cumulus cells indicates that these factors produced by cumulus cells may be responsible for maximal EGFR activation during oocyte maturation, precluding any further effect of exogenous r-hEGF. Additionally, these results illustrate the potential futility of exogenous supplementation of IVM medium without prior knowledge of pathway activity.

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Essential role of ubiquitin C‐terminal hydrolases UCHL1 and UCHL3 in mammalian oocyte maturation

Mtango

Sutovsky

VandeVoort

et al. 2012

Journal Cellular Physiology

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Ubiquitin C-terminal hydrolases (UCHs) comprise a family of deubiquitinating enzymes that play a role in the removal of multi-ubiquitin chains from proteins that are posttranslationally modified by ubiquitination to be targeted for proteolysis by the 26S proteasome. The UCH-enzymes also generate free monomeric ubiquitin from precursor multi-ubiquitin chains and, in some instances, may rescue ubiquitinated proteins from degradation. This study examined the roles of two oocyte-expressed UCHs, UCHL1 and UCHL3 in murine and rhesus monkey oocyte maturation. The Uchl1 and Uchl3 mRNAs were highly expressed in GV and MII oocytes, and were associated with the oocyte cortex (UCHL1) and meiotic spindle (UCHL3). Microinjection of the UCH-family enzyme inhibitor, ubiquitin-aldehyde (UBAL) to GV oocytes prevented oocyte meiotic progression beyond metaphase I in a majority of treated oocytes and caused spindle and first polar body anomalies. Injection of antibodies against UCHL3 disrupted oocyte maturation and caused meiotic anomalies, including abnormally long meiotic spindles. A selective, cell permeant inhibitor of UCHL3, 4, 5, 6, 7-Tetrachloroidan-1, 3-dione also caused meiotic defects and chromosome misalignment. Cortical granule localization in the oocyte cortex was disrupted by UBAL injected after oocyte maturation. We conclude that the activity of oocyte UCHs contributes to oocyte maturation by regulating the oocyte cortex and meiotic spindle.

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Growth hormone and in vitro maturation of rhesus macaque oocytes and subsequent embryo development

Abstract: The presence of GH receptors along with increased cumulus expansion and embryos progressing to the 9-16 cell stage supports the hypothesis that r-hGH may be involved in oocyte maturation.

Cited by 37 publications

References 66 publications

Differential Effects of Follistatin on Nonhuman Primate Oocyte Maturation and Pre-Implantation Embryo Development In Vitro1

Differential Effects of Follistatin on Nonhuman Primate Oocyte Maturation and Pre-Implantation Embryo Development In Vitro1

Role for cumulus cell-produced EGF-like ligands during primate oocyte maturation in vitro

Essential role of ubiquitin C‐terminal hydrolases UCHL1 and UCHL3 in mammalian oocyte maturation

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