Effects of Genistein on Oxidative Injury in Endothelial Cells

Abstract

SummaryThe aim of this study was to test the hypothesis that genistein protects vascular endothelial cells against the pro-atherosclerotic stressor, oxidized low-density lipoprotein (ox-LDL), by inducing antioxidant enzymes and preventing apoptosis. Human umbilical cord-derived endothelial cells (ECV 304) were incubated with genistein (10-100 mol/L), the radical scavenging antioxidant vitamin E ( ␣ -tocopherol, 50 mol/L), or vehicle for 24 h and then were incubated with ox-LDL for an additional 24 h. Subsequently, antioxidant enzyme activities, lipid peroxidation, adhesion to monocytes, cell morphology, viability and apoptotic index were assessed. Ox-LDL decreased superoxide dismutase and glutathione peroxidase activities in endothelial cells and caused lipid peroxidation, adhesion to monocytes, morphological injury and apoptosis ( p Ͻ 0.05). These effects were prevented by vitamin E and dose-dependently by genistein ( p Ͻ 0.05). Further, this effect of genistein is associated with maintenance of antioxidant enzyme activities and inhibition of lipid peroxidation.

Effect of free or protein‐associated soy isoflavones on the antioxidant status in rats

“…This is attributed to their phenolic ring structure and multiple hydroxyl groups, especially on the A ring. Genistein, one of the isoflavones in soy with three hydroxyl groups, has been found to be a strong antioxidant that can decrease low‐density lipoprotein oxidation in vitro 15. Additionally, Cai and Wei16 observed that dietary administration of genistein (50 and 250 mg kg −1 ) for 30 days significantly increased the activities of antioxidant enzymes in the small intestine and skin of SENCAR mice.…”

Genistein as a neuroprotective antioxidant attenuates redox imbalance induced by β‐amyloid peptides 25–35 in PC12 cells

“…The naturally occurring antioxidant compound GEN presents at high concentration in soybean, and is the most active component of soy isoflavones. GEN has many physiological functions and some of the beneficial effects have been attributed to its antioxidant properties (Sonee et al, 2004; Ho et al, 2003; Huang et al, 2008).…”

Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy

“…Soy protein intake potentially modulates inflammatory response among type 2 DN (12). Some data are available regarding the beneficial effects of soy products on inflammatory markers (13–18), coagulation (14), and oxidative stress indices (15). …”