Effects of genetically modified T2A-1 rice on faecal microflora of rats during 90 day supplementation

Yang, Yuan; Xu, Wentao; Luo, Yunbo; Liu, Haiyan; Lü, Jiao; Su, Chunyuan; Huang, Kunlun

doi:10.1002/jsfa.4421

Cited by 13 publications

(15 citation statements)

References 40 publications

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“…However, it has been severely criticized by some scientists:it emphasizes chemical composition at the expense of biological, toxicological, and immunological tests; it is not clear what level of similarity can make the GM and non-GM food substantially equivalent (Bhattacharya et al 2013) or it does not define the point at which a food is no longer substantially equivalent (Picone et al 2011). Subchronic rodent toxicology studies have been conducted with whole grains and processed feed fractions obtained from numerous GM crops in accordance with guidelines of repeated dose 90-day oral toxicity study in rodents (Cao et al 2011;Yuan et al 2011;Arjó et al 2012;Liu et al 2012;Tang et al 2012).…”

Section: Discussionmentioning

confidence: 99%

A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague–Dawley rats

Song

Zou

et al. 2014

Transgenic Res

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Bacillus thuringiensis (Bt) transgenic rice line (mfb-MH86) expressing a synthetic cry1Ab gene can be protected against feeding damage from Lepidopteran insects, including Sesamia inferens, Chilo suppressalis, Tryporyza incertulas and Cnaphalocrocis medinalis. Rice flour from mfb-MH86 and its near-isogenic control MH86 was separately formulated into rodent diets at concentrations of 17.5, 35 and 70 % (w/w) for a 90-day feeding test with rats, and all of the diets were nutritionally balanced. In this study, the responses of rats fed diets containing mfb-MH86 were compared to those of rats fed flour from MH86. Overall health, body weight and food consumption were comparable between groups fed diets containing mfb-MH86 and MH86. Blood samples were collected prior to sacrifice and a few significant differences (p \ 0.05) were observed in haematological and biochemical parameters between rats fed genetically modified (GM) and non-GM diets. However, the values of these parameters were within the normal ranges of values for rats of this age and sex, thus not considered treatment related. In addition, upon sacrifice a large number of organs were weighed, macroscopic and histopathological examinations were performed with only minor changes to report. In conclusion, these results demonstrated that no toxic effect was observed in the conditions of the experiment, based on the different parameters assessed. GM rice mfb-MH86 is as safe and nutritious as non-GM rice.

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Section: Discussionmentioning

confidence: 99%

A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague–Dawley rats

Song

Zou

et al. 2014

Transgenic Res

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“…However, GM rice expressing lectin or PAT protein has been reported to cause significant changes to the intestinal microflora of rats (Poulsen et al, 2007a, b). Yet, when selected intestinal bacteria (Yuan et al, 2011) and microflora composition (Yuan et al, 2013) of rats fed T2A-1 rice were analyzed by real-time PCR or DGGE, no significant effects were observed. Therefore, in this study, we investigated the effects of feeding Bt-rice for 42 days on the microbiota of broiler chickens.…”

Section: Introductionmentioning

confidence: 93%

“…Historically, several methods such as culturebased approaches, denaturing-gradient gel electrophoresis (DGGE), and real-time polymerase chain reaction (PCR) have been used widely to analyze the composition of intestinal microflora. However, culture-based methods could identify only 10-40% of bacteria (Tannock et al, 2001(Tannock et al, , 2002, co-migration of phylogenetically heterogeneous bands reduced the sensitivity of DGGE (Yang et al, 2000;Sekiguchi et al, 2001), and real-time PCR could only be used to monitor selected bacterial taxa (Matsuki et al, 2004;Yuan et al, 2011). The advent of high-throughput sequencing has offered a new way of investigating the microbial community.…”

Section: Introductionmentioning

confidence: 99%

High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric <i>Bacillus thuringiensis</i> Protein

Zhang

et al. 2018

J. Poult. Sci.

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Many types of Bacillus thuringiensis (Bt)-crops are being grown worldwide, triggering concerns about their potential impact on humans and livestock. To ensure better yield and food safety in China, an attempt has been made to develop Bt-rice targeting a broad range of insects. We aimed to investigate whether feeding genetically modified rice expressing the Bt chimeric Cry1Ac/Cry1Ab protein has any effects on the intestinal microbiota of broilers. Broilers were fed either Bt-rice or its unmodified isogenic parent line for 42 days, and total DNA was isolated from cecum contents for high-throughput sequencing of the 16S rRNA gene. In total, 1,241,005 reads, assigned to 12 phyla, 31 families, and 48 genera were generated. No significant differences were observed in the relative abundance of organisms identified among the major phyla, families, and genera, except for two less abundant families, Thermoanaerobacteraceae and Peptostreptococcaceae, and two less abundant genera, Anaerotruncus and Gelria. The results were in agreement with those from culture-based analysis and Biolog EcoPlates. These results illustrate that feeding Bt-rice has no adverse effects on the broiler intestinal microbiota and provide sufficient support for the food safety of Bt-rice.

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“…Fecal bacteria DNA was extracted from 0.2-0.25 g samples with a QIAamp DNA stool extraction mini kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. DNA with optical density ratio OD260/OD280 ranging from 1.8 to 2.0 was taken for quantification (8).…”

Section: Dna Extractionmentioning

confidence: 99%

“…There is a slight difference of GI microbiota according to their histories and lifestyles, including diet (7). However, for health individual, the proportion of the main beneficial intestinal bacterium and potential pathotens is in the normal range (8). As beneficial intestinal bacteria, Bifidobacterium has abilities, including modulation of the GI tract, acetic acid production, antagonism against other pathogenic bacteria, and tolerance to diet allergens (9).…”

Section: Introductionmentioning

confidence: 99%

The effect of genetically modified Lactobacillus plantarum 590 on the gut health of sprague–dawley rats

Liu

Yang

et al. 2012

IUBMB Life

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Lp was a generally recognized as safe microorganism. Lactobacillus plantarum 590 was obtained by inserting nisI gene into Lp genome to help it tolerate higher concentration nisin. As the unintended effects of the genetically modified microorganism (GMM) are the most important barriers to the progress of GMM, we have performed a useful exploration to establish a new in vivo evaluation model for GMM from the point of view of intestinal health. In this study, Sprague–Dawley rats were orally administered with Lp 590 and Lp for 4 weeks. Fecal samples were collected to determine the number of beneficial bacteria Bifidobacterium and harmful bacteria Clostridium perfringens. Denaturing gradient gel electrophoresis was used to detect the bacterial profiles of every group. Fecal enzyme activities and short‐chain fatty acids as main metabolites were also examined. Real time PCR (RT‐PCR) and immunohistochemistry were used to analyze two proteins (ZO‐1 and occludin) and secretory immunoglobulin A to detect intestinal permeability and mucosal immunity, gut permeability and gut mucosal immunity were analyzed to see whether GM Lp 590 can induce changes of the gut health when compared with non‐GM Lp group, andeventually we concluded that there is no significant difference between GM Lp 590‐fed group and non‐GM Lp‐fed group. The conclusion of gut health test was comparable withthat from traditional subchronic test. Evaluation of intestinal health will be a new approach of assessing the safety of GMM. © 2012 IUBMB Life, 64(7): 617–627, 2012.

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Effects of genetically modified T2A-1 rice on faecal microflora of rats during 90 day supplementation

Abstract: No adverse effects on the numbers of specific bacteria in rat faeces were observed as a result of GMR feeding. The real-time PCR method is recommended in further studies on the composition and dynamics of the intestinal bacteria community for better safety assessment of GM materials.

Cited by 13 publications

References 40 publications

A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague–Dawley rats

A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague–Dawley rats

High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric <i>Bacillus thuringiensis</i> Protein

The effect of genetically modified Lactobacillus plantarum 590 on the gut health of sprague–dawley rats

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